温度: 293 K / 手法: 脂質キュービック相法 詳細: 30 or 50 nL bolus overlaid with 600 nL precipitant solution using either a Mosquito LCP robot (SPT Labtech) in CrystalDirect plate-2. The crystallisation condition is made of 42.3% PEG 400, ...詳細: 30 or 50 nL bolus overlaid with 600 nL precipitant solution using either a Mosquito LCP robot (SPT Labtech) in CrystalDirect plate-2. The crystallisation condition is made of 42.3% PEG 400, 110 mM potassium citrate, 100 mM HEPES pH 7.0. 30-150 nL of Tb-XO4 soaking solution with a final conc. of 5 - 25 mM was delivered onto LCP crystallisation drop. Crystallisation experiments were carried out at the HTX facility of EMBL Grenoble. LCP bolus were harvested automatically at cryogenic condition using CrystalDirect technology.