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- PDB-6ybu: Crystal structure of a native BcsE (349-523) RQ complex with c-di... -

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Basic information

Entry
Database: PDB / ID: 6ybu
TitleCrystal structure of a native BcsE (349-523) RQ complex with c-di-GMP and ATP bound
Components(Bacterial cellulose secretion regulator ...) x 3
KeywordsSIGNALING PROTEIN / Bacterial biofilms / Bacterial cellulose / Bacterial secretion system / ATP binding protein / c-di-GMP binding protein
Function / homology
Function and homology information


bacterial cellulose biosynthetic process / cellulose biosynthetic process / negative regulation of cell division / cyclic-di-GMP binding / cytoplasmic side of plasma membrane / cell division / ATP hydrolysis activity / ATP binding / cytoplasm / cytosol
Similarity search - Function
Cellulose biosynthesis protein BcsE / Cellulose biosynthesis protein BcsE / Protein YhjR / Cellulose biosynthesis protein BcsR-like / Cellulose synthase operon protein BcsQ / Cellulose biosynthesis protein BcsQ / : / P-loop containing nucleotide triphosphate hydrolases / Rossmann fold / P-loop containing nucleoside triphosphate hydrolase ...Cellulose biosynthesis protein BcsE / Cellulose biosynthesis protein BcsE / Protein YhjR / Cellulose biosynthesis protein BcsR-like / Cellulose synthase operon protein BcsQ / Cellulose biosynthesis protein BcsQ / : / P-loop containing nucleotide triphosphate hydrolases / Rossmann fold / P-loop containing nucleoside triphosphate hydrolase / 3-Layer(aba) Sandwich / Alpha Beta
Similarity search - Domain/homology
ADENOSINE-5'-TRIPHOSPHATE / Chem-C2E / Cell division protein / Cellulose biosynthesis protein BcsR / Protein YhjR / Cellulose biosynthesis protein BcsQ / Cyclic di-GMP binding protein BcsE
Similarity search - Component
Biological speciesEscherichia coli (E. coli)
MethodX-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2.49 Å
AuthorsAbidi, W. / Zouhir, S. / Roche, S. / Krasteva, P.V.
Funding support5items
OrganizationGrant numberCountry
ATIP-Avenir
European Research Council (ERC)BioMatrix, ERC StG #757507
Centre National de la Recherche Scientifique (CNRS)
Institute for Integrative Biology of the Cell (I2BC)
European Institute of Chemistry and Biology (IECB)
CitationJournal: Sci Adv / Year: 2021
Title: Architecture and regulation of an enterobacterial cellulose secretion system.
Authors: Wiem Abidi / Samira Zouhir / Meryem Caleechurn / Stéphane Roche / Petya Violinova Krasteva /
Abstract: Many free-living and pathogenic enterobacteria secrete biofilm-promoting cellulose using a multicomponent, envelope-embedded Bcs secretion system under the control of intracellular second messenger c- ...Many free-living and pathogenic enterobacteria secrete biofilm-promoting cellulose using a multicomponent, envelope-embedded Bcs secretion system under the control of intracellular second messenger c-di-GMP. The molecular understanding of system assembly and cellulose secretion has been largely limited to the crystallographic studies of a distantly homologous BcsAB synthase tandem and a low-resolution reconstruction of an assembled macrocomplex that encompasses most of the inner membrane and cytosolic subunits and features an atypical layered architecture. Here, we present cryo-EM structures of the assembled Bcs macrocomplex, as well as multiple crystallographic snapshots of regulatory Bcs subcomplexes. The structural and functional data uncover the mechanism of asymmetric secretion system assembly and periplasmic crown polymerization and reveal unexpected subunit stoichiometry, multisite c-di-GMP recognition, and ATP-dependent regulation.
History
DepositionMar 17, 2020Deposition site: PDBE / Processing site: PDBE
Revision 1.0Feb 24, 2021Provider: repository / Type: Initial release
Revision 1.1Jan 24, 2024Group: Data collection / Database references / Refinement description
Category: chem_comp_atom / chem_comp_bond ...chem_comp_atom / chem_comp_bond / database_2 / pdbx_initial_refinement_model
Item: _database_2.pdbx_DOI / _database_2.pdbx_database_accession
Revision 1.2Oct 1, 2025Group: Advisory / Derived calculations / Structure summary
Category: pdbx_entry_details / pdbx_validate_symm_contact ...pdbx_entry_details / pdbx_validate_symm_contact / struct_conn / struct_conn_type
Item: _pdbx_entry_details.has_protein_modification

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
A: Bacterial cellulose secretion regulator BcsQ
B: Bacterial cellulose secretion regulator BcsQ
C: Bacterial cellulose secretion regulator BcsR
D: Bacterial cellulose secretion regulator BcsR
E: Bacterial cellulose secretion regulator BcsE, residues 349-523
F: Bacterial cellulose secretion regulator BcsE, residues 349-523
G: Bacterial cellulose secretion regulator BcsQ
H: Bacterial cellulose secretion regulator BcsQ
I: Bacterial cellulose secretion regulator BcsR
J: Bacterial cellulose secretion regulator BcsR
K: Bacterial cellulose secretion regulator BcsE, residues 349-523
L: Bacterial cellulose secretion regulator BcsE, residues 349-523
hetero molecules


Theoretical massNumber of molelcules
Total (without water)230,42428
Polymers222,77512
Non-polymers7,64916
Water4,089227
1
A: Bacterial cellulose secretion regulator BcsQ
B: Bacterial cellulose secretion regulator BcsQ
C: Bacterial cellulose secretion regulator BcsR
D: Bacterial cellulose secretion regulator BcsR
E: Bacterial cellulose secretion regulator BcsE, residues 349-523
F: Bacterial cellulose secretion regulator BcsE, residues 349-523
hetero molecules


Theoretical massNumber of molelcules
Total (without water)115,21214
Polymers111,3886
Non-polymers3,8258
Water905
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
2
G: Bacterial cellulose secretion regulator BcsQ
H: Bacterial cellulose secretion regulator BcsQ
I: Bacterial cellulose secretion regulator BcsR
J: Bacterial cellulose secretion regulator BcsR
K: Bacterial cellulose secretion regulator BcsE, residues 349-523
L: Bacterial cellulose secretion regulator BcsE, residues 349-523
hetero molecules


Theoretical massNumber of molelcules
Total (without water)115,21214
Polymers111,3886
Non-polymers3,8258
Water1086
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Unit cell
Length a, b, c (Å)107.990, 72.570, 161.250
Angle α, β, γ (deg.)90.000, 98.370, 90.000
Int Tables number4
Space group name H-MP1211
Space group name HallP2yb
Symmetry operation#1: x,y,z
#2: -x,y+1/2,-z

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Components

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Bacterial cellulose secretion regulator ... , 3 types, 12 molecules ABGHCDIJEFKL

#1: Protein
Bacterial cellulose secretion regulator BcsQ / Cellulose biosynthesis protein BcsQ / Cellulose synthase operon protein YhjQ / Cellulose synthase / ...Cellulose biosynthesis protein BcsQ / Cellulose synthase operon protein YhjQ / Cellulose synthase / putative / Bacterial cellulose synthesis subunit Q


Mass: 27960.832 Da / Num. of mol.: 4
Source method: isolated from a genetically manipulated source
Details: Bacterial cellulose synthesis subunit Q. / Source: (gene. exp.) Escherichia coli (E. coli)
Gene: bcsQ, yhjQ, A8C65_00290, ACU57_05335, BMT91_17060, BON75_10030, BvCmsHHP019_01723, BvCmsSINP011_05061, C2U48_15650, D3O91_11725, D9H68_14440, D9I18_15755, D9I97_13990, DAH34_22885, DAH37_19450, ...Gene: bcsQ, yhjQ, A8C65_00290, ACU57_05335, BMT91_17060, BON75_10030, BvCmsHHP019_01723, BvCmsSINP011_05061, C2U48_15650, D3O91_11725, D9H68_14440, D9I18_15755, D9I97_13990, DAH34_22885, DAH37_19450, E2127_16420, E2128_18010, E2129_18145, E2134_17810, EAI42_04085, EC1094V2_71, NCTC10429_00778, NCTC11022_03734, NCTC9058_01652
Production host: Escherichia coli BL21(DE3) (bacteria) / References: UniProt: A0A0B1KWQ0, UniProt: P0DP92*PLUS
#2: Protein
Bacterial cellulose secretion regulator BcsR / FIG004405: Putative cytoplasmic protein / Protein YhjR / Protein of uncharacterized function ...FIG004405: Putative cytoplasmic protein / Protein YhjR / Protein of uncharacterized function (DUF2629) / Bacterial cellulose synthesis subunit R


Mass: 7452.367 Da / Num. of mol.: 4
Source method: isolated from a genetically manipulated source
Details: The coding region corresponding to the BcsRQ tandem was cloned into the pProEx-Htb with a cleavable N-terminal hexahistidine tag to BcsR. The sample were purified and crystallised without ...Details: The coding region corresponding to the BcsRQ tandem was cloned into the pProEx-Htb with a cleavable N-terminal hexahistidine tag to BcsR. The sample were purified and crystallised without the His-tag. The GPMGS correspond to the Nter additional residues remaining after cleavage. Bacterial cellulose synthesis subunit R.
Source: (gene. exp.) Escherichia coli (E. coli)
Gene: yhjR, A6V01_14365, A8C65_00295, A9R57_18690, AC789_1c39040, ACN002_3620, ACN68_06780, ACN81_08775, ACU57_05330, ACU90_15155, AM270_16530, AM464_10395, AMK83_17075, AML07_15400, APZ14_14840, ...Gene: yhjR, A6V01_14365, A8C65_00295, A9R57_18690, AC789_1c39040, ACN002_3620, ACN68_06780, ACN81_08775, ACU57_05330, ACU90_15155, AM270_16530, AM464_10395, AMK83_17075, AML07_15400, APZ14_14840, AUQ13_21010, AUS26_05355, AW106_19925, BANRA_02188, BANRA_03431, BANRA_04333, BANRA_04566, BB545_03705, BHS81_21135, BHS87_19835, BJJ90_00965, BK292_24575, BMT91_17065, BN17_34711, BOH76_20305, BON63_23095, BON69_12460, BON71_17720, BON72_13310, BON76_21180, BON94_21140, BON95_21275, BTQ06_14355, BUE81_10045, BvCms12BK_03867, BvCms2454_00062, BvCms28BK_00015, BvCmsHHP001_04915, BvCmsHHP019_01722, BvCmsHHP056_04702, BvCmsKKP036_02918, BvCmsKKP061_02263, BvCmsKSNP073_03410, BvCmsKSNP081_04400, BvCmsKSP011_02716, BvCmsKSP024_02867, BvCmsKSP026_02341, BvCmsKSP045_00352, BvCmsKSP067_01939, BvCmsNSNP036_04602, BvCmsNSP006_05307, BvCmsNSP047_03956, BvCmsNSP072_04054, BvCmsOUP014_03413, BvCmsSINP011_05062, BvCmsSIP019_02407, BvCmsSIP044_03516, BVL39_08345, BW690_01625, BZL31_14290, C2U48_15645, C5N07_21610, C5P01_24180, C6669_10230, C7235_01450, C7B02_19840, C9098_17010, C9114_22000, C9141_22075, C9160_22970, C9162_26275, C9182_22470, C9201_19395, C9306_17625, C9E25_16190, C9Z03_00095, C9Z28_19620, C9Z37_15395, C9Z39_13945, C9Z69_16745, C9Z89_13935, CA593_08480, CI641_010930, COD30_23820, COD46_13475, CR538_01230, CRD98_22705, CRM83_21580, CWS33_18425, D0X26_22825, D2184_20785, D2185_17650, D3821_09240, D3Y67_04435, D6T60_22230, D9D20_19605, D9E35_13135, D9H68_14435, D9I18_15750, D9I97_13985, D9J11_18915, D9J44_18935, D9K48_10615, D9K54_10550, DAH18_11545, DAH30_06135, DAH32_17855, DAH34_22880, DAH37_19445, DBQ99_02170, DEN89_25155, DEN97_17470, DEO04_20390, DEO19_17140, DIV22_07035, DJ503_16110, DL545_01700, DL800_25095, DP277_20930, DQF57_09495, DQO13_19235, DS732_25515, DTL43_22125, DXT69_13975, DXT71_18500, DXT73_16115, E0I42_16815, E0J34_09730, E0K84_22700, E2119_10885, E2127_16425, E2128_18015, E2129_18150, E2134_17815, E2135_13675, E2855_04489, E2863_04566, E3B71_14240, E5P22_13630, E5P28_15170, E5P37_17785, E5S35_16770, E5S47_16125, EAI42_04080, EC1094V2_70, EC3234A_62c00180, EC3426_04694, EC95NR1_02922, ED600_16775, EEP23_03690, EHH55_25990, EJC75_16880, EKI52_16535, EL75_0168, EL79_0179, EL80_0171, ELT20_13340, ELV08_08625, EPT01_13645, EQ825_24275, ERS085365_03384, ERS085374_03939, ERS085379_03461, ERS085416_01810, ERS139211_03246, EXX13_15900, EXX71_20125, EXX78_22145, EYD11_00910, EYY78_10840, F0312_08835, F1E03_18480, F1E19_10145, F7F23_20655, F7F29_18335, FORC82_0211, FQ915_11140, FQR64_01380, FRV13_18835, FTV90_03830, FTV92_19380, FV293_20875, FWK02_16270, FY127_16255, HW43_22525, NCTC10090_03288, NCTC10418_00365, NCTC10429_00777, NCTC10865_00350, NCTC11022_03735, NCTC11126_00353, NCTC11181_02507, NCTC11341_02195, NCTC13148_03788, NCTC8009_01140, NCTC8179_05669, NCTC8500_00017, NCTC8960_02842, NCTC8985_04684, NCTC9045_00297, NCTC9055_02150, NCTC9058_01653, NCTC9062_02927, NCTC9111_00632, NCTC9703_04725, NCTC9706_02488, PGD_04560, PU06_03455, RG28_22775, RK56_020175, RX35_03299, SAMEA3472043_03895, SAMEA3472055_04880, SAMEA3472070_03765, SAMEA3472114_02155, SAMEA3484427_00198, SAMEA3484429_02974, SAMEA3752553_00829, SAMEA3752557_03916, SAMEA3752559_00583, SAMEA3753064_01978, SAMEA3753290_02280, SAMEA3753300_03978, SK85_03853, UN86_19365, WQ89_12680, WR15_14750
Production host: Escherichia coli BL21(DE3) (bacteria) / References: UniProt: J7QAC9, UniProt: P0ADJ3*PLUS
#3: Protein
Bacterial cellulose secretion regulator BcsE, residues 349-523 / Cellulose biosynthesis protein BcsE / Bacterial cellulose synthesis subunit E


Mass: 20280.561 Da / Num. of mol.: 4
Source method: isolated from a genetically manipulated source
Details: The coding region of BcsE corresponding to residue 349 to 523 was cloned into a modified pRSF-Duet to express the truncated protein without a tag. The first additional MGSM residues come ...Details: The coding region of BcsE corresponding to residue 349 to 523 was cloned into a modified pRSF-Duet to express the truncated protein without a tag. The first additional MGSM residues come from the cloning. Bacterial cellulose synthesis subunit E.
Source: (gene. exp.) Escherichia coli (strain K12) (bacteria)
Strain: K12 / Gene: bcsE, yhjS, b3536, JW3504 / Production host: Escherichia coli BL21(DE3) (bacteria) / References: UniProt: P37657

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Non-polymers , 4 types, 243 molecules

#4: Chemical
ChemComp-ATP / ADENOSINE-5'-TRIPHOSPHATE


Mass: 507.181 Da / Num. of mol.: 4 / Source method: obtained synthetically / Formula: C10H16N5O13P3 / Feature type: SUBJECT OF INVESTIGATION / Comment: ATP, energy-carrying molecule*YM
#5: Chemical
ChemComp-MG / MAGNESIUM ION


Mass: 24.305 Da / Num. of mol.: 4 / Source method: obtained synthetically / Formula: Mg / Feature type: SUBJECT OF INVESTIGATION
#6: Chemical
ChemComp-C2E / 9,9'-[(2R,3R,3aS,5S,7aR,9R,10R,10aS,12S,14aR)-3,5,10,12-tetrahydroxy-5,12-dioxidooctahydro-2H,7H-difuro[3,2-d:3',2'-j][1,3,7,9,2,8]tetraoxadiphosphacyclododecine-2,9-diyl]bis(2-amino-1,9-dihydro-6H-purin-6-one) / c-di-GMP / Cyclic diguanosine monophosphate


Mass: 690.411 Da / Num. of mol.: 8 / Source method: obtained synthetically / Formula: C20H24N10O14P2 / Feature type: SUBJECT OF INVESTIGATION
#7: Water ChemComp-HOH / water


Mass: 18.015 Da / Num. of mol.: 227 / Source method: isolated from a natural source / Formula: H2O

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Details

Has ligand of interestY
Has protein modificationN

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Experimental details

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Experiment

ExperimentMethod: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

CrystalDensity Matthews: 2.81 Å3/Da / Density % sol: 56.17 %
Crystal growTemperature: 277.4 K / Method: vapor diffusion, hanging drop
Details: 100 mM Tris-HCl pH 9.0, 22-25% PEG 6000, 500 mM LiCl

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Data collection

DiffractionMean temperature: 100 K / Serial crystal experiment: N
Diffraction sourceSource: SYNCHROTRON / Site: SOLEIL / Beamline: PROXIMA 2 / Wavelength: 0.980057954788 Å
DetectorType: DECTRIS EIGER2 X 9M / Detector: PIXEL / Date: Nov 15, 2017
RadiationProtocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelengthWavelength: 0.980057954788 Å / Relative weight: 1
ReflectionResolution: 2.49→50 Å / Num. obs: 86110 / % possible obs: 98.5 % / Redundancy: 6.9 % / Biso Wilson estimate: 52.41 Å2 / CC1/2: 0.995 / Net I/σ(I): 11.45
Reflection shellResolution: 2.49→2.64 Å / Redundancy: 6.77 % / Mean I/σ(I) obs: 1.26 / Num. unique obs: 12814 / CC1/2: 0.542 / % possible all: 91.4

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Processing

Software
NameVersionClassification
PHENIX1.17rc2_3619refinement
Cootmodel building
PHASERphasing
XDSdata scaling
XDSdata processing
XDSdata reduction
RefinementMethod to determine structure: MOLECULAR REPLACEMENT
Starting model: 6YAR, 6TJ0

6yar

6tj0


Resolution: 2.49→47.71 Å / SU ML: 0.3472 / Cross valid method: FREE R-VALUE / σ(F): 1.34 / Phase error: 24.7448
RfactorNum. reflection% reflection
Rfree0.2249 4331 5.03 %
Rwork0.1808 --
obs0.1831 86096 98.76 %
Solvent computationShrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å
Displacement parametersBiso mean: 61.92 Å2
Refinement stepCycle: LAST / Resolution: 2.49→47.71 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms13815 0 496 227 14538
Refine LS restraints
Refine-IDTypeDev idealNumber
X-RAY DIFFRACTIONf_bond_d0.002114668
X-RAY DIFFRACTIONf_angle_d0.490120069
X-RAY DIFFRACTIONf_chiral_restr0.03812261
X-RAY DIFFRACTIONf_plane_restr0.00312827
X-RAY DIFFRACTIONf_dihedral_angle_d13.61668933
LS refinement shell
Resolution (Å)Rfactor RfreeNum. reflection RfreeRfactor RworkNum. reflection RworkRefine-ID% reflection obs (%)
2.49-2.520.42981200.3591823X-RAY DIFFRACTION67.16
2.52-2.550.35091330.30052721X-RAY DIFFRACTION99.86
2.55-2.580.30321510.27972771X-RAY DIFFRACTION99.76
2.58-2.610.30421590.26212712X-RAY DIFFRACTION99.79
2.61-2.640.31071310.25182731X-RAY DIFFRACTION99.79
2.64-2.680.30291610.25692720X-RAY DIFFRACTION99.86
2.68-2.720.29181540.2532730X-RAY DIFFRACTION99.69
2.72-2.760.3131350.242744X-RAY DIFFRACTION99.93
2.76-2.80.32551350.25422732X-RAY DIFFRACTION99.83
2.8-2.850.35761490.2562740X-RAY DIFFRACTION99.9
2.85-2.90.28571410.24552771X-RAY DIFFRACTION99.86
2.9-2.950.33451410.23052721X-RAY DIFFRACTION99.79
2.95-3.010.3031450.22222756X-RAY DIFFRACTION99.72
3.01-3.070.27461420.20992712X-RAY DIFFRACTION99.83
3.07-3.130.28771450.20562759X-RAY DIFFRACTION99.93
3.13-3.210.28681440.20022718X-RAY DIFFRACTION99.83
3.21-3.290.23041480.20342814X-RAY DIFFRACTION99.9
3.29-3.380.26481420.20082712X-RAY DIFFRACTION99.96
3.38-3.480.23691460.18312772X-RAY DIFFRACTION99.97
3.48-3.590.2411440.16462723X-RAY DIFFRACTION99.9
3.59-3.720.21281450.15882778X-RAY DIFFRACTION99.86
3.72-3.870.20451440.16682757X-RAY DIFFRACTION99.76
3.87-4.040.1881460.15252769X-RAY DIFFRACTION99.9
4.04-4.250.19481450.15312754X-RAY DIFFRACTION99.93
4.25-4.520.19131460.13922785X-RAY DIFFRACTION99.86
4.52-4.870.17641450.13782776X-RAY DIFFRACTION99.86
4.87-5.360.18261450.14572756X-RAY DIFFRACTION99.93
5.36-6.130.20991480.17692801X-RAY DIFFRACTION99.86
6.13-7.720.20011480.17362825X-RAY DIFFRACTION99.93
7.72-47.710.14331530.14852882X-RAY DIFFRACTION99.64
Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

IDL11 (°2)L12 (°2)L13 (°2)L22 (°2)L23 (°2)L33 (°2)S11 (Å °)S12 (Å °)S13 (Å °)S21 (Å °)S22 (Å °)S23 (Å °)S31 (Å °)S32 (Å °)S33 (Å °)T11 (Å2)T12 (Å2)T13 (Å2)T22 (Å2)T23 (Å2)T33 (Å2)Origin x (Å)Origin y (Å)Origin z (Å)
10.3333130204860.45545326805-0.8125929157021.296164427960.4425608522151.13016502708-0.0734897676072-0.5480882648950.01106879006110.0995184258865-0.0903210624082-0.262978780968-0.04288514863760.3875839265494.63294147256E-50.3540507823630.0277299284645-0.04515956852880.6400158982560.150510729470.458523040696-24.1906718051-9.8072098709261.2539760895
20.7605583311540.0191726952566-0.3457827270410.4858340943191.269371563022.442630627480.0799619351499-0.2326658395890.01130743425280.1498833318290.183649594033-0.2161715341150.2322752542690.797705032369-0.0002218294202130.3987397698980.0660924836707-0.08337958716030.8605931391980.1733684291420.539627906967-17.5321601493-11.231530838261.8197692702
30.376413187933-0.17090241172-0.7205631129441.08295359073-0.0815160807182.02439412427-0.0949331459750.0742216139765-0.214998859908-0.0183147658023-0.1115049948620.02742353721330.0917983057080.342760245709-0.0001519020413940.3140953423170.04458570585380.0374249153970.3868026867140.1216806620890.413908293247-29.2072447291-15.654801892941.8719937964
40.9451084329651.051693835720.2390537609480.9456800955560.4836535866630.625932250044-0.178467535892-0.1996498855660.03793559278870.0332259073490.09671060985650.4394408212660.1003401348270.00084605346043-0.0001421370949790.3851454974930.06456873706360.06023478059690.4433626789560.0951703302420.396951337951-38.4257708965-14.840328412956.4768469447
50.906438355854-0.5594993398260.7491238393390.890092047426-0.3167577207960.6010931738090.07270571253750.2755011215550.0358217973505-0.080768216572-0.273010015063-0.15346281175-0.09576133859170.451489074352-0.010480700250.33120205812-0.02070540119370.01846187916540.6119646870230.1911135459420.455459840795-19.3949979892.6538071361227.8123656774
60.514054794341-0.564804076366-0.2874106407651.357720121061.193627056031.21547601382-0.152666916745-0.04120098425640.107976318161-0.01717236838460.213520985996-0.178600824664-0.2313243110730.48585474915-0.004544318698290.291151771425-0.0932672886455-0.01013812611060.8024718298210.2302978924270.593441255073-11.2029146353.9426171094334.1606791159
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Refinement TLS group
IDRefine-IDRefine TLS-IDSelection details
1X-RAY DIFFRACTION1chain 'A' and (resid 2 through 71 )
2X-RAY DIFFRACTION2chain 'A' and (resid 72 through 125 )
3X-RAY DIFFRACTION3chain 'A' and (resid 126 through 202 )
4X-RAY DIFFRACTION4chain 'A' and (resid 203 through 241 )
5X-RAY DIFFRACTION5chain 'B' and (resid 2 through 88 )
6X-RAY DIFFRACTION6chain 'B' and (resid 89 through 130 )
7X-RAY DIFFRACTION7chain 'B' and (resid 131 through 202 )
8X-RAY DIFFRACTION8chain 'B' and (resid 203 through 241 )
9X-RAY DIFFRACTION9chain 'C' and (resid 32 through 42 )
10X-RAY DIFFRACTION10chain 'C' and (resid 43 through 60 )
11X-RAY DIFFRACTION11chain 'D' and (resid 33 through 42 )
12X-RAY DIFFRACTION12chain 'D' and (resid 43 through 61 )
13X-RAY DIFFRACTION13chain 'E' and (resid 348 through 358 )
14X-RAY DIFFRACTION14chain 'E' and (resid 359 through 435 )
15X-RAY DIFFRACTION15chain 'E' and (resid 436 through 485 )
16X-RAY DIFFRACTION16chain 'E' and (resid 486 through 497 )
17X-RAY DIFFRACTION17chain 'E' and (resid 498 through 515 )
18X-RAY DIFFRACTION18chain 'F' and (resid 352 through 368 )
19X-RAY DIFFRACTION19chain 'F' and (resid 369 through 380 )
20X-RAY DIFFRACTION20chain 'F' and (resid 381 through 410 )
21X-RAY DIFFRACTION21chain 'F' and (resid 411 through 425 )
22X-RAY DIFFRACTION22chain 'F' and (resid 426 through 435 )
23X-RAY DIFFRACTION23chain 'F' and (resid 436 through 485 )
24X-RAY DIFFRACTION24chain 'F' and (resid 486 through 497 )
25X-RAY DIFFRACTION25chain 'F' and (resid 498 through 507 )
26X-RAY DIFFRACTION26chain 'F' and (resid 508 through 517 )
27X-RAY DIFFRACTION27chain 'G' and (resid 2 through 67 )
28X-RAY DIFFRACTION28chain 'G' and (resid 68 through 141 )
29X-RAY DIFFRACTION29chain 'G' and (resid 142 through 202 )
30X-RAY DIFFRACTION30chain 'G' and (resid 203 through 241 )
31X-RAY DIFFRACTION31chain 'H' and (resid 2 through 88 )
32X-RAY DIFFRACTION32chain 'H' and (resid 89 through 125 )
33X-RAY DIFFRACTION33chain 'H' and (resid 126 through 202 )
34X-RAY DIFFRACTION34chain 'H' and (resid 203 through 240 )
35X-RAY DIFFRACTION35chain 'I' and (resid 31 through 43 )
36X-RAY DIFFRACTION36chain 'I' and (resid 44 through 60 )
37X-RAY DIFFRACTION37chain 'J' and (resid 28 through 42 )
38X-RAY DIFFRACTION38chain 'J' and (resid 43 through 60 )
39X-RAY DIFFRACTION39chain 'K' and (resid 352 through 368 )
40X-RAY DIFFRACTION40chain 'K' and (resid 369 through 380 )
41X-RAY DIFFRACTION41chain 'K' and (resid 381 through 435 )
42X-RAY DIFFRACTION42chain 'K' and (resid 436 through 485 )
43X-RAY DIFFRACTION43chain 'K' and (resid 486 through 497 )
44X-RAY DIFFRACTION44chain 'K' and (resid 498 through 517 )
45X-RAY DIFFRACTION45chain 'L' and (resid 354 through 368 )
46X-RAY DIFFRACTION46chain 'L' and (resid 369 through 390 )
47X-RAY DIFFRACTION47chain 'L' and (resid 391 through 475 )
48X-RAY DIFFRACTION48chain 'L' and (resid 476 through 491 )
49X-RAY DIFFRACTION49chain 'L' and (resid 492 through 515 )

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