Database of articles cited by EMDB/PDB/SASBDB data

Keywords
Structure methods	All X-ray diffraction NMR electron microscopy small angle scattering neutron diffraction fiber diffraction powder diffraction infrared spectroscopy EPR fluorescence transfer theoretical model Hybrid
Author
Journal
IF	>30 >20 >10 >5 all

Title	Architecture and regulation of an enterobacterial cellulose secretion system.
Journal, issue, pages	Sci Adv, Vol. 7, Issue 5, Year 2021
Publish date	Jan 27, 2021
Authors	Wiem Abidi / Samira Zouhir / Meryem Caleechurn / Stéphane Roche / Petya Violinova Krasteva /
PubMed Abstract	Many free-living and pathogenic enterobacteria secrete biofilm-promoting cellulose using a multicomponent, envelope-embedded Bcs secretion system under the control of intracellular second messenger c- ...Many free-living and pathogenic enterobacteria secrete biofilm-promoting cellulose using a multicomponent, envelope-embedded Bcs secretion system under the control of intracellular second messenger c-di-GMP. The molecular understanding of system assembly and cellulose secretion has been largely limited to the crystallographic studies of a distantly homologous BcsAB synthase tandem and a low-resolution reconstruction of an assembled macrocomplex that encompasses most of the inner membrane and cytosolic subunits and features an atypical layered architecture. Here, we present cryo-EM structures of the assembled Bcs macrocomplex, as well as multiple crystallographic snapshots of regulatory Bcs subcomplexes. The structural and functional data uncover the mechanism of asymmetric secretion system assembly and periplasmic crown polymerization and reveal unexpected subunit stoichiometry, multisite c-di-GMP recognition, and ATP-dependent regulation.
External links	Sci Adv / PubMed:33563593 / PubMed Central
Methods	EM (single particle) / X-ray diffraction
Resolution	1.59 - 5.9 Å
Structure data	10799 6yg8 EMDB-10799, PDB-6yg8: Cryo-EM structure of a BcsB pentamer in the context of an assembled Bcs macrocomplex Method: EM (single particle) / Resolution: 3.0 Å EMDB-11356: Structure of detergent-extracted full-length E.coli BcsB Method: EM (single particle) / Resolution: 5.9 Å EMDB-11836: Cryo-EM density map corresponding to BcsRQAB subcomplex Method: EM (single particle) / Resolution: 4.5 Å PDB-6yar: Crystal structure of a Selenium-derivatized complex of the bacterial cellulose secretion regulators BcsR and BcsQ, crystallized in the presence of AppCp Method: X-RAY DIFFRACTION / Resolution: 1.9 Å PDB-6yay: Crystal structure of a Selenium-derivatized complex of the bacterial cellulose secretion regulators BcsR and BcsQ, crystallized in the presence of ADP Method: X-RAY DIFFRACTION / Resolution: 2.09 Å PDB-6yb3: Crystal structure of a native BcsRQ complex purified and crystallized in the absence of nucleotide Method: X-RAY DIFFRACTION / Resolution: 1.59 Å PDB-6yb5: Orthorhombic crystal structure of a native BcsRQ complex crystallized in the presence of ADP Method: X-RAY DIFFRACTION / Resolution: 1.59 Å PDB-6ybb: Crystal structure of a native BcsE (217-523) - BcsR-BcsQ (R156E mutant) complex with c-di-GMP and ATP bound Method: X-RAY DIFFRACTION / Resolution: 2.9 Å PDB-6ybu: Crystal structure of a native BcsE (349-523) RQ complex with c-di-GMP and ATP bound Method: X-RAY DIFFRACTION / Resolution: 2.49 Å
Chemicals	ChemComp-MG: Unknown entry ChemComp-ATP: ADENOSINE-5'-TRIPHOSPHATE / ATP, energy-carrying moleculeYM / Adenosine triphosphate ChemComp-HOH: WATER / Water ChemComp-C2E: 9,9'-[(2R,3R,3aS,5S,7aR,9R,10R,10aS,12S,14aR)-3,5,10,12-tetrahydroxy-5,12-dioxidooctahydro-2H,7H-difuro[3,2-d:3',2'-j][1,3,7,9,2,8]tetraoxadiphosphacyclododecine-2,9-diyl]bis(2-amino-1,9-dihydro-6H-purin-6-one) / Cyclic di-GMP ChemComp-GOL*: GLYCEROL / Glycerol
Source	escherichia coli (E. coli) escherichia coli (strain k12) (bacteria)
Keywords	SIGNALING PROTEIN / Bacterial biofilms / Bacterial cellulose / Bacterial secretion system / ATP binding protein / c-di-GMP binding protein / regulator protein