+Open data
-Basic information
Entry | Database: PDB / ID: 6xwx | ||||||
---|---|---|---|---|---|---|---|
Title | Crystal structure of the Tof1-Csm3 complex | ||||||
Components |
| ||||||
Keywords | REPLICATION / Fork Protection Complex / Replisome / Cohesion establishment / S phase checkpoint / Armadillo repeat | ||||||
Function / homology | Function and homology information : / DNA replication checkpoint signaling / negative regulation of DNA replication / meiotic cell cycle / cell cycle / DNA damage response / nucleus Similarity search - Function | ||||||
Biological species | Chaetomium thermophilum (fungus) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 3.1 Å | ||||||
Authors | Grabarczyk, D.B. | ||||||
Funding support | Germany, 1items
| ||||||
Citation | Journal: Nucleic Acids Res. / Year: 2020 Title: Crystal structure and interactions of the Tof1-Csm3 (Timeless-Tipin) fork protection complex. Authors: Grabarczyk, D.B. | ||||||
History |
|
-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
---|
-Downloads & links
-Download
PDBx/mmCIF format | 6xwx.cif.gz | 387.6 KB | Display | PDBx/mmCIF format |
---|---|---|---|---|
PDB format | pdb6xwx.ent.gz | 318.7 KB | Display | PDB format |
PDBx/mmJSON format | 6xwx.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/xw/6xwx ftp://data.pdbj.org/pub/pdb/validation_reports/xw/6xwx | HTTPS FTP |
---|
-Related structure data
Related structure data | 5mqiS S: Starting model for refinement |
---|---|
Similar structure data |
-Links
-Assembly
Deposited unit |
| ||||||||
---|---|---|---|---|---|---|---|---|---|
1 |
| ||||||||
2 |
| ||||||||
3 |
| ||||||||
Unit cell |
|
-Components
#1: Protein | Mass: 69540.414 Da / Num. of mol.: 3 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719) (fungus) Gene: CTHT_0029130 / Production host: Escherichia coli (E. coli) / References: UniProt: G0S825 #2: Protein | Mass: 12895.146 Da / Num. of mol.: 3 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719) (fungus) Gene: CTHT_0011090 / Production host: Escherichia coli (E. coli) / References: UniProt: G0S0S7 |
---|
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
---|
-Sample preparation
Crystal | Density Matthews: 2.9 Å3/Da / Density % sol: 57.6 % |
---|---|
Crystal grow | Temperature: 293 K / Method: vapor diffusion, sitting drop Details: 0.2 M Potassium Acetate, 0.1 M Tris-HCl pH 7.5-8.5, 4-8% PEG 20000 |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N |
---|---|
Diffraction source | Source: SYNCHROTRON / Site: PETRA III, EMBL c/o DESY / Beamline: P14 (MX2) / Wavelength: 0.98 Å |
Detector | Type: DECTRIS EIGER X 16M / Detector: PIXEL / Date: May 27, 2019 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.98 Å / Relative weight: 1 |
Reflection | Resolution: 3.09→76.74 Å / Num. obs: 27164 / % possible obs: 89.8 % / Redundancy: 7 % / CC1/2: 0.996 / Rpim(I) all: 0.078 / Net I/σ(I): 7.1 |
Reflection shell | Resolution: 3.09→3.524 Å / Mean I/σ(I) obs: 1.2 / Num. unique obs: 1359 / CC1/2: 0.365 / Rpim(I) all: 0.737 |
-Processing
Software |
| ||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: 5MQI Resolution: 3.1→45.73 Å / Cor.coef. Fo:Fc: 0.909 / Cor.coef. Fo:Fc free: 0.895 / Cross valid method: THROUGHOUT / SU Rfree Blow DPI: 0.741
| ||||||||||||||||||||
Displacement parameters | Biso mean: 123.41 Å2
| ||||||||||||||||||||
Refine analyze | Luzzati coordinate error obs: 0.64 Å | ||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 3.1→45.73 Å
| ||||||||||||||||||||
LS refinement shell | Resolution: 3.1→3.39 Å
|