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- PDB-6x04: Nup133 (aa55-481) from S. cerevisiae bound by VHH-SAN5 -

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Basic information

Entry
Database: PDB / ID: 6x04
TitleNup133 (aa55-481) from S. cerevisiae bound by VHH-SAN5
Components
  • Nucleoporin NUP133
  • VHH-SAN5
KeywordsSTRUCTURAL PROTEIN / Nucleoporin / Nanobody
Function / homology
Function and homology information


mRNA export from nucleus in response to heat stress / nuclear pore localization / transcription-dependent tethering of RNA polymerase II gene DNA at nuclear periphery / nuclear pore outer ring / telomere tethering at nuclear periphery / Transport of Mature mRNA derived from an Intron-Containing Transcript / post-transcriptional tethering of RNA polymerase II gene DNA at nuclear periphery / Regulation of HSF1-mediated heat shock response / tRNA export from nucleus / SUMOylation of SUMOylation proteins ...mRNA export from nucleus in response to heat stress / nuclear pore localization / transcription-dependent tethering of RNA polymerase II gene DNA at nuclear periphery / nuclear pore outer ring / telomere tethering at nuclear periphery / Transport of Mature mRNA derived from an Intron-Containing Transcript / post-transcriptional tethering of RNA polymerase II gene DNA at nuclear periphery / Regulation of HSF1-mediated heat shock response / tRNA export from nucleus / SUMOylation of SUMOylation proteins / structural constituent of nuclear pore / SUMOylation of RNA binding proteins / SUMOylation of chromatin organization proteins / nucleocytoplasmic transport / silent mating-type cassette heterochromatin formation / poly(A)+ mRNA export from nucleus / nuclear pore / subtelomeric heterochromatin formation / protein import into nucleus / nuclear envelope / double-strand break repair / cellular response to heat / nuclear membrane / chromosome, telomeric region / positive regulation of DNA-templated transcription / negative regulation of transcription by RNA polymerase II / positive regulation of transcription by RNA polymerase II / nucleus / cytosol
Similarity search - Function
Nuclear pore complex protein Nup133-like / Nucleoporin, Nup133/Nup155-like, N-terminal / Nup133 N terminal like / YVTN repeat-like/Quinoprotein amine dehydrogenase / 7 Propeller / Methylamine Dehydrogenase; Chain H / WD40/YVTN repeat-like-containing domain superfamily / Mainly Beta
Similarity search - Domain/homology
Biological speciesSaccharomyces cerevisiae (brewer's yeast)
Vicugna pacos (alpaca)
MethodX-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2.68 Å
AuthorsNordeen, S.A. / Schwartz, T.U.
Funding support United States, 1items
OrganizationGrant numberCountry
National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)GM077537 United States
CitationJournal: Nat Commun / Year: 2020
Title: Yeast Nup84-Nup133 complex structure details flexibility and reveals conservation of the membrane anchoring ALPS motif.
Authors: Nordeen, S.A. / Turman, D.L. / Schwartz, T.U.
History
DepositionMay 15, 2020Deposition site: RCSB / Processing site: RCSB
Revision 1.0Dec 9, 2020Provider: repository / Type: Initial release
Revision 1.1Oct 18, 2023Group: Data collection / Database references / Refinement description
Category: chem_comp_atom / chem_comp_bond ...chem_comp_atom / chem_comp_bond / database_2 / pdbx_initial_refinement_model
Item: _database_2.pdbx_DOI / _database_2.pdbx_database_accession

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
A: Nucleoporin NUP133
B: VHH-SAN5
C: Nucleoporin NUP133
D: VHH-SAN5
E: Nucleoporin NUP133
F: VHH-SAN5
G: Nucleoporin NUP133
H: VHH-SAN5
I: Nucleoporin NUP133
J: VHH-SAN5
K: Nucleoporin NUP133
L: VHH-SAN5


Theoretical massNumber of molelcules
Total (without water)367,48812
Polymers367,48812
Non-polymers00
Water27015
1


  • Idetical with deposited unit
  • defined by author
  • Evidence: gel filtration
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Unit cell
Length a, b, c (Å)85.799, 204.761, 205.833
Angle α, β, γ (deg.)90.000, 90.000, 90.000
Int Tables number19
Space group name H-MP212121
Space group name HallP2ac2ab
Symmetry operation#1: x,y,z
#2: x+1/2,-y+1/2,-z
#3: -x,y+1/2,-z+1/2
#4: -x+1/2,-y,z+1/2

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Components

#1: Protein
Nucleoporin NUP133 / Nuclear pore protein NUP133


Mass: 48493.730 Da / Num. of mol.: 6
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast)
Strain: ATCC 204508 / S288c / Gene: NUP133, RAT3, YKR082W, YKR402 / Production host: Escherichia coli (E. coli) / References: UniProt: P36161
#2: Antibody
VHH-SAN5


Mass: 12754.293 Da / Num. of mol.: 6
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Vicugna pacos (alpaca) / Production host: Escherichia coli (E. coli)
#3: Water ChemComp-HOH / water


Mass: 18.015 Da / Num. of mol.: 15 / Source method: isolated from a natural source / Formula: H2O

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Experimental details

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Experiment

ExperimentMethod: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

CrystalDensity Matthews: 2.51 Å3/Da / Density % sol: 51 %
Crystal growTemperature: 291 K / Method: vapor diffusion, hanging drop
Details: 15% PEG MME 2,000, 0.1M potassium chloride, 0.1M Tris/HCl pH 8

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Data collection

DiffractionMean temperature: 100 K / Serial crystal experiment: N
Diffraction sourceSource: SYNCHROTRON / Site: APS / Beamline: 24-ID-C / Wavelength: 0.9791 Å
DetectorType: PSI PILATUS 6M / Detector: PIXEL / Date: Jun 17, 2017
RadiationProtocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelengthWavelength: 0.9791 Å / Relative weight: 1
ReflectionResolution: 2.68→49.68 Å / Num. obs: 100374 / % possible obs: 98.7 % / Redundancy: 8.6 % / Biso Wilson estimate: 72.6 Å2 / CC1/2: 0.999 / Rpim(I) all: 0.035 / Net I/σ(I): 29.1
Reflection shellResolution: 2.68→2.8 Å / Redundancy: 8.3 % / Mean I/σ(I) obs: 1.49 / Num. unique obs: 9937 / CC1/2: 0.567 / Rpim(I) all: 0.684 / % possible all: 99.1

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Processing

Software
NameVersionClassification
PHENIX1.18_3845refinement
HKL-2000data reduction
HKL-2000data scaling
PHENIXphasing
RefinementMethod to determine structure: MOLECULAR REPLACEMENT
Starting model: 4Q9T

4q9t


Resolution: 2.68→49.68 Å / SU ML: 0.4285 / Cross valid method: FREE R-VALUE / σ(F): 1.33 / Phase error: 29.6567
Stereochemistry target values: GeoStd + Monomer Library + CDL v1.2
RfactorNum. reflection% reflection
Rfree0.2614 1999 1.99 %
Rwork0.2435 98267 -
obs0.2439 100266 98.06 %
Solvent computationShrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL
Displacement parametersBiso mean: 88.69 Å2
Refinement stepCycle: LAST / Resolution: 2.68→49.68 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms20000 0 0 15 20015
Refine LS restraints
Refine-IDTypeDev idealNumber
X-RAY DIFFRACTIONf_bond_d0.004720356
X-RAY DIFFRACTIONf_angle_d0.647227693
X-RAY DIFFRACTIONf_chiral_restr0.04973285
X-RAY DIFFRACTIONf_plane_restr0.00353481
X-RAY DIFFRACTIONf_dihedral_angle_d17.43616833
LS refinement shell
Resolution (Å)Rfactor RfreeNum. reflection RfreeRfactor RworkNum. reflection RworkRefine-ID% reflection obs (%)
2.68-2.750.41691350.38076306X-RAY DIFFRACTION89.8
2.75-2.820.32171400.34496799X-RAY DIFFRACTION96.32
2.82-2.910.35321370.31467024X-RAY DIFFRACTION99.13
2.91-30.34081460.30247018X-RAY DIFFRACTION99.11
3-3.110.32531420.2917020X-RAY DIFFRACTION99.16
3.11-3.230.31641430.28647074X-RAY DIFFRACTION99.42
3.23-3.380.29551430.28027080X-RAY DIFFRACTION99.34
3.38-3.560.29221390.25296940X-RAY DIFFRACTION97.52
3.56-3.780.26771450.24397114X-RAY DIFFRACTION99.53
3.78-4.070.27431420.24837114X-RAY DIFFRACTION99.56
4.07-4.480.23431470.20347139X-RAY DIFFRACTION99.43
4.48-5.130.17741430.18887042X-RAY DIFFRACTION97.58
5.13-6.460.27491470.22747258X-RAY DIFFRACTION99.72
6.46-49.680.23741500.24537339X-RAY DIFFRACTION97.12
Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

IDL11 (°2)L12 (°2)L13 (°2)L22 (°2)L23 (°2)L33 (°2)S11 (Å °)S12 (Å °)S13 (Å °)S21 (Å °)S22 (Å °)S23 (Å °)S31 (Å °)S32 (Å °)S33 (Å °)T11 (Å2)T12 (Å2)T13 (Å2)T22 (Å2)T23 (Å2)T33 (Å2)Origin x (Å)Origin y (Å)Origin z (Å)
12.35648278661-0.0843070487864-0.9498161606847.51014701225-3.765412326776.92005657668-0.1908411678880.9060229368380.420107608835-1.47204522537-0.4464308453720.124984907364-0.727227264851-0.1271208775870.3541504920981.532185499480.197185326864-0.3335192977040.9839177961640.2204384338430.8297316701329.9998922039340.7157587730.0136382696
24.126142126631.56009138047-0.9104911047073.63266825212-1.817126951076.21227154121-0.05522109538890.82681360195-0.216526656638-1.036628613050.132566289262-0.04177253387880.302089126826-0.276218995501-0.05881289364180.7414009498570.2711239597410.03304725346110.64621475366-0.03878658783290.32197910868513.896438950122.08622551739.3293762927
34.762949570620.2794279448880.5353914089748.083292652082.183002451041.708776706610.02974381613130.421955780651-0.10662916717-0.715078945213-0.254258572691-0.473727296041-0.07241997486590.2539370455330.2088424928830.587857136790.158840199995-0.05631000985760.5532027276130.1362232470710.40821727072217.055575224122.083462858648.2346459187
42.024261204673.094344280160.1434157706168.20793491732-1.800941912689.22026420303-0.0299919441748-0.0929217880190.38262844586-0.269399954022-0.201213361932-0.0964945547726-0.1250445704810.2711375752560.1809574690780.4853207320430.154680087004-0.06756490887950.466179027610.06894168754370.45388745367317.952409450537.619497924255.3181326749
54.81422092421.509252477130.9847935718957.915192826082.799141462266.11376280015-0.2091782423280.135390067096-0.262751721598-0.525062550410.02664771963690.380536651548-0.2309554933270.1119475953410.1634961575830.5007665609630.150764814991-0.01239976480050.4960119643530.003610244203860.45265265650413.7916874838-2.0154862486655.8260076611
63.810068181744.43992108795-4.923708266199.24716394672-2.612225072898.776665423460.910037280841-1.96268748977-0.6669796203320.999012512463-0.879268253252-0.758333539478-0.4671985514221.44307480822-0.1733160881750.4551498380610.142990636557-0.0571446861810.971377657234-0.07880345112990.5404202279514.2660426084-1.2996033332964.9193238051
72.168567948320.462401389178-0.1784004454683.80145682419-0.4451099703691.64677827287-0.06797885300480.0484632588097-0.107772920122-0.456245648787-0.216850084949-0.2615071761690.180455049570.1407555652710.2737953397990.5897968510510.163300459598-0.06842935096320.541239171243-0.05470357375390.60139792930116.5393594092-1.8939971731354.1623789658
82.965221154561.494265901612.880083905824.779438726290.4315547687156.420022226330.948158582584-0.478373669003-0.7218069590031.39624440747-0.390844298289-0.5834125891451.393981368270.615465021179-0.3989486092381.11517916010.0327551527865-0.2481089010151.076357369180.1666676338990.67465304465842.94950779614.49648889063119.590786024
90.2679756257730.218089100847-1.156910958426.15888110264-4.345389724596.797428116791.48701264435-0.940615365516-1.154208546210.501606426591-0.704311956087-0.7850109717691.19095116275-0.881611943331-0.3291237902571.14993256389-0.31722602661-0.5745683032131.34778731910.2081847426630.6830600571648.992593004114.1538687371128.027012115
102.55458611461-1.93563658971-1.394395881523.59537441538-0.555381324254.071046516090.126161401266-1.258663537170.4016624238672.136003203660.102410302272-0.857715953258-0.3148308217570.636952551486-0.206791911090.83164669237-0.237099196254-0.2616193514951.130629219520.08499347448120.49637998063648.610532910524.4135394619131.267042535
111.260923118060.4457415776580.1040844564827.294715399871.378041486586.283616508780.255494052151-0.8813442807380.4593310089050.539574225909-0.121106871071-0.07422392647960.0289617180511-0.105261401552-0.1047255936940.416224864416-0.0531075686541-0.08305251387550.893871543538-0.04171438481710.45270896845844.255420352628.5519092307118.522631753
124.044226148151.83043625781.618358405411.885585611881.808961981382.11657012219-0.0479104017662-0.388508709013-0.0265504872343-0.1613361860190.0317925608443-0.122657167027-0.265384516325-0.04215195085420.0323413854550.22308241202-0.0275040359722-0.0488186387960.4963700985850.04576302012670.38708915163437.411961682126.6354056476101.799652094
134.73131735959-0.417489405021.189098309855.914178592610.07243566820157.32032445602-0.064681316228-0.5068141297490.4704184171570.209456504074-0.0186128034495-0.5614181338850.1650206264330.2123557958360.08158288093130.2070531440.0208378610238-0.07410038726980.3226252921290.04571689143380.42436038823834.574316721417.0219132141101.102597948
140.570082629851-1.44657396236-0.3661721527094.064691122820.1944892783815.85821241351-0.03838083894340.137753603184-0.0747083381244-0.1368794653890.191056877042-0.452964487744-0.1128923522140.292447242917-0.153266545340.2024111345140.014307451346-0.1034458147680.520050782692-0.01925539968430.41214587117637.118070764419.622265690694.8786517962
150.9118220548230.6314921975251.690152756812.358871783361.66505824225.905403112560.141455961516-0.314871080147-0.672760723722-0.08457904344720.00965527885981-0.6923346276040.447577025773-0.524618920956-0.07869331964950.509533278438-0.0679327849428-0.1727433396170.5887384983750.1167090919690.82546608314535.81819740959.18185571364105.831238226
169.491837386173.025399417964.193577034899.01270372673-0.4572902154794.15629460798-0.6943557178111.96887269139-1.3285736761-1.89777385820.261416875745-1.241464587020.2469771383921.619346792020.2396502318970.677513526122-0.08077948670560.006009001839620.902854844154-0.09044170101690.80119993780341.76297325926.6313383298394.9821605406
174.933157056663.117442595415.48152275886.600214872543.902056927696.978821837810.595915532179-0.173965892782-0.8552743785431.49332289145-0.375740233356-0.689202291781.090482114560.365346083614-0.2157144117930.904161189135-0.0290333469736-0.4435906505130.8733583295970.1480576925570.9109539139.94078623412.56527213453113.378435499
183.83226382056-1.70880876140.7257489638159.71358680716-0.3784482435195.85811450978-0.2401456231080.703473142947-0.124035054963-0.4050356494340.1536522576980.1871513595230.4405154967320.03919684655960.07496084737520.373308162342-0.0781411128701-0.03043902653830.6251652410920.03249758146190.29878339385921.146266524527.129499424970.3905424336
196.389793761333.581745392693.137987097727.72010865776-4.099034050067.55057551879-0.396641891888-0.610374958128-1.25612628732-2.38711499308-0.47185965502-1.250855101242.353851342982.268140679560.7001990956440.715047425430.06626245648330.1145764180820.597760103452-0.02543791892190.77002557255227.4820429249.5887089222277.5840707124
200.989419742124-1.771077933450.7174104648574.96239297842-2.10408339043.74006513995-0.107758390271-0.2250363218850.09069113988840.8643584630990.03586924072920.337461129449-0.335659488947-0.001352265195070.04944170651540.145763289655-0.0403287259646-0.0920593134670.446368946143-0.01940940365430.48988517465420.021360184426.97424798584.2291351113
212.74452739277-1.66648328043-2.611900599862.05518258345-0.4120760235156.548688916150.1411341961530.114118510012-1.12448525566-0.277007774673-0.8188651231110.9087386175670.469473592197-0.1274496914470.7682995769280.366398090575-0.049444429508-0.1018277843910.4585507322950.04694680890650.75102650822816.735140905717.485104277176.7171968514
220.3611405388211.166496317170.04533859052553.876477120730.1925447303781.96148556727-0.1975165056680.2237792881910.0575615162301-0.3175779439870.2038779324850.118676005076-0.145396164899-0.214949739629-0.01664398931010.201634296097-0.00884051477228-0.0545529506770.549457556322-0.0009286858774440.5104814977920.732307493726.215697060777.7080538845
233.82337534864-5.304039809042.761330017657.26484268166-4.265981695965.344932489330.2843348954060.7318435181540.6397791900930.067405111102-0.875727548511-1.086467430550.1079915583961.158070828750.5512423326370.2679652346150.00760218944303-0.03754786218670.5392169433330.01592097119580.66964576508128.523112990724.594814539678.491500703
242.255606138410.74682603492-0.07028784025333.36561325261-0.6439053531744.253998626690.3967882637340.685602048944-1.20013333776-0.165982243745-0.0907951348453-0.5421054963630.8282424292150.0319023936188-0.2749354730741.010548874490.0897852926939-0.1932877713230.660458419821-0.2925445099671.1328250161323.0988599322-39.429537125147.7964796949
253.084384542-1.000183128860.8250198045026.321130674241.429118539354.232875521910.2273998434480.149003685068-0.915907121296-0.193103938353-0.261959597949-0.7804352675030.7616395079230.3054007228180.04857667007061.065225506890.181219280496-0.2068420302330.591249046810.02120352221351.5142968187234.098653085-47.004542621364.732625363
261.62339066636-1.91276382175-1.637737622565.63202457311.487369509798.080806215360.217473408652-0.4376379259610.0578618053260.05025944539590.0362722705241-0.3224413210120.5680629523570.383051719404-0.2770265490570.605919453025-0.0733116281915-0.1893315237420.5240941055860.03179090905920.8331661531824.8187631669-24.221220560373.6920698818
273.014595793530.4642336928062.25422921534.257072262520.58882777797.243851486410.2587051837060.110058787825-0.352572509048-0.0800739964825-0.213943759768-0.3544099516110.322002526087-0.00342776281556-0.09501348471150.4823448492730.1396260700090.02348448266570.41916882643-0.01659491020140.67036227834124.9938795531-25.074748899460.0160490291
284.09328469710.0422662247865-0.9471956348225.13146066690.4034090369828.719118606770.391029067308-0.343731268087-0.08144491515541.13201996018-0.0472457376870.00496006935992-0.2790485074611.11508799913-0.2201900246410.53035079576-0.107145419187-0.1917245950310.619079058262-0.1135550697990.8505011868331.6457293087-4.6105639282997.2832845535
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763.137328741962.04275976455-0.1962805429241.382875035630.634358082196.213187508310.1721730275020.2625249762021.20983909677-0.937254044206-0.2489052933710.354216230344-0.755063256577-0.6973117292180.1433285249851.387319808920.23948985747-0.2350968433410.6677517651070.209560385821.0252117069814.105701793750.429809475345.9993954307
Refinement TLS group

Refine-ID: X-RAY DIFFRACTION

IDRefine TLS-IDSelection detailsAuth asym-IDLabel asym-IDAuth seq-IDLabel seq-ID
11chain 'G' and (resid 169 through 279 )GG169 - 27965 - 152
22chain 'G' and (resid 280 through 331 )GG280 - 331153 - 204
33chain 'G' and (resid 332 through 382 )GG332 - 382205 - 255
44chain 'G' and (resid 383 through 481 )GG383 - 481256 - 334
55chain 'H' and (resid 2 through 65 )HH2 - 651 - 64
66chain 'H' and (resid 66 through 75 )HH66 - 7565 - 74
77chain 'H' and (resid 76 through 117 )HH76 - 11775 - 116
88chain 'I' and (resid 61 through 103 )II61 - 1031 - 36
99chain 'I' and (resid 104 through 163 )II104 - 16337 - 62
1010chain 'I' and (resid 164 through 197 )II164 - 19763 - 84
1111chain 'I' and (resid 198 through 279 )II198 - 27985 - 153
1212chain 'I' and (resid 280 through 331 )II280 - 331154 - 204
1313chain 'I' and (resid 332 through 357 )II332 - 357205 - 230
1414chain 'I' and (resid 358 through 382 )II358 - 382231 - 255
1515chain 'I' and (resid 383 through 420 )II383 - 420256 - 285
1616chain 'I' and (resid 421 through 443 )II421 - 443286 - 308
1717chain 'I' and (resid 444 through 481 )II444 - 481309 - 346
1818chain 'J' and (resid 2 through 25 )JJ2 - 251 - 24
1919chain 'J' and (resid 26 through 32 )JJ26 - 3225 - 31
2020chain 'J' and (resid 33 through 65 )JJ33 - 6532 - 64
2121chain 'J' and (resid 66 through 75 )JJ66 - 7565 - 74
2222chain 'J' and (resid 76 through 100 )JJ76 - 10075 - 99
2323chain 'J' and (resid 101 through 117 )JJ101 - 117100 - 116
2424chain 'K' and (resid 61 through 140 )KK61 - 1401 - 54
2525chain 'K' and (resid 141 through 312 )KK141 - 31255 - 177
2626chain 'K' and (resid 313 through 357 )KK313 - 357178 - 222
2727chain 'K' and (resid 358 through 481 )KK358 - 481223 - 328
2828chain 'L' and (resid 2 through 22 )LL2 - 221 - 21
2929chain 'L' and (resid 23 through 45 )LL23 - 4522 - 44
3030chain 'L' and (resid 46 through 76 )LL46 - 7645 - 75
3131chain 'L' and (resid 77 through 117 )LL77 - 11776 - 116
3232chain 'A' and (resid 61 through 163 )AA61 - 1631 - 66
3333chain 'A' and (resid 164 through 192 )AA164 - 19267 - 91
3434chain 'A' and (resid 193 through 266 )AA193 - 26692 - 152
3535chain 'A' and (resid 267 through 331 )AA267 - 331153 - 217
3636chain 'A' and (resid 332 through 481 )AA332 - 481218 - 367
3737chain 'B' and (resid 2 through 9 )BB2 - 91 - 8
3838chain 'B' and (resid 10 through 22 )BB10 - 229 - 21
3939chain 'B' and (resid 23 through 32 )BB23 - 3222 - 31
4040chain 'B' and (resid 33 through 40 )BB33 - 4032 - 39
4141chain 'B' and (resid 41 through 58 )BB41 - 5840 - 57
4242chain 'B' and (resid 59 through 65 )BB59 - 6558 - 64
4343chain 'B' and (resid 66 through 76 )BB66 - 7665 - 75
4444chain 'B' and (resid 77 through 100 )BB77 - 10076 - 99
4545chain 'B' and (resid 101 through 117 )BB101 - 117100 - 116
4646chain 'C' and (resid 61 through 86 )CC61 - 861 - 21
4747chain 'C' and (resid 87 through 140 )CC87 - 14022 - 55
4848chain 'C' and (resid 141 through 200 )CC141 - 20056 - 83
4949chain 'C' and (resid 201 through 227 )CC201 - 22784 - 110
5050chain 'C' and (resid 228 through 266 )CC228 - 266111 - 130
5151chain 'C' and (resid 267 through 298 )CC267 - 298131 - 160
5252chain 'C' and (resid 299 through 331 )CC299 - 331161 - 190
5353chain 'C' and (resid 332 through 357 )CC332 - 357191 - 216
5454chain 'C' and (resid 358 through 382 )CC358 - 382217 - 241
5555chain 'C' and (resid 383 through 412 )CC383 - 412242 - 268
5656chain 'C' and (resid 413 through 454 )CC413 - 454269 - 310
5757chain 'C' and (resid 455 through 481 )CC455 - 481311 - 337
5858chain 'D' and (resid 2 through 22 )DD2 - 221 - 21
5959chain 'D' and (resid 23 through 52 )DD23 - 5222 - 51
6060chain 'D' and (resid 53 through 76 )DD53 - 7652 - 75
6161chain 'D' and (resid 77 through 100 )DD77 - 10076 - 99
6262chain 'D' and (resid 101 through 117 )DD101 - 117100 - 116
6363chain 'E' and (resid 60 through 218 )EE60 - 2181 - 98
6464chain 'E' and (resid 219 through 266 )EE219 - 26699 - 132
6565chain 'E' and (resid 267 through 357 )EE267 - 357133 - 215
6666chain 'E' and (resid 358 through 427 )EE358 - 427216 - 274
6767chain 'E' and (resid 428 through 481 )EE428 - 481275 - 315
6868chain 'F' and (resid 2 through 17 )FF2 - 171 - 16
6969chain 'F' and (resid 18 through 52 )FF18 - 5217 - 51
7070chain 'F' and (resid 53 through 58 )FF53 - 5852 - 57
7171chain 'F' and (resid 59 through 81 )FF59 - 8158 - 80
7272chain 'F' and (resid 82 through 89 )FF82 - 8981 - 88
7373chain 'F' and (resid 90 through 100 )FF90 - 10089 - 99
7474chain 'F' and (resid 101 through 108 )FF101 - 108100 - 107
7575chain 'F' and (resid 109 through 117 )FF109 - 117108 - 116
7676chain 'G' and (resid 59 through 168 )GG59 - 1681 - 64

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