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- PDB-6wtc: Crystal Structure of the Second Form of the Co-factor Complex of ... -

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Basic information

Entry
Database: PDB / ID: 6wtc
TitleCrystal Structure of the Second Form of the Co-factor Complex of NSP7 and the C-terminal Domain of NSP8 from SARS CoV-2
Components
  • Non-structural protein 7
  • Non-structural protein 8
KeywordsVIRAL PROTEIN / SARS CoV-2 / co-factor for RNA polymerase NSP12 / Structural Genomics / Center for Structural Genomics of Infectious Diseases / CSGID
Function / homology
Function and homology information


protein guanylyltransferase activity / RNA endonuclease activity producing 3'-phosphomonoesters, hydrolytic mechanism / mRNA guanylyltransferase activity / 5'-3' RNA helicase activity / Lyases; Phosphorus-oxygen lyases / Assembly of the SARS-CoV-2 Replication-Transcription Complex (RTC) / symbiont-mediated suppression of host cytoplasmic pattern recognition receptor signaling pathway via inhibition of TBK1 activity / Maturation of replicase proteins / TRAF3-dependent IRF activation pathway / ISG15-specific peptidase activity ...protein guanylyltransferase activity / RNA endonuclease activity producing 3'-phosphomonoesters, hydrolytic mechanism / mRNA guanylyltransferase activity / 5'-3' RNA helicase activity / Lyases; Phosphorus-oxygen lyases / Assembly of the SARS-CoV-2 Replication-Transcription Complex (RTC) / symbiont-mediated suppression of host cytoplasmic pattern recognition receptor signaling pathway via inhibition of TBK1 activity / Maturation of replicase proteins / TRAF3-dependent IRF activation pathway / ISG15-specific peptidase activity / Transcription of SARS-CoV-2 sgRNAs / snRNP Assembly / Translation of Replicase and Assembly of the Replication Transcription Complex / Replication of the SARS-CoV-2 genome / Hydrolases; Acting on ester bonds; Exoribonucleases producing 5'-phosphomonoesters / double membrane vesicle viral factory outer membrane / host cell endoplasmic reticulum-Golgi intermediate compartment / SARS coronavirus main proteinase / 5'-3' DNA helicase activity / 3'-5'-RNA exonuclease activity / host cell endosome / symbiont-mediated degradation of host mRNA / mRNA guanylyltransferase / symbiont-mediated suppression of host ISG15-protein conjugation / G-quadruplex RNA binding / symbiont-mediated suppression of host toll-like receptor signaling pathway / symbiont-mediated suppression of host cytoplasmic pattern recognition receptor signaling pathway via inhibition of IRF3 activity / omega peptidase activity / mRNA (guanine-N7)-methyltransferase / methyltransferase cap1 / SARS-CoV-2 modulates host translation machinery / host cell Golgi apparatus / symbiont-mediated suppression of host NF-kappaB cascade / symbiont-mediated perturbation of host ubiquitin-like protein modification / DNA helicase / methyltransferase cap1 activity / ubiquitinyl hydrolase 1 / cysteine-type deubiquitinase activity / mRNA 5'-cap (guanine-N7-)-methyltransferase activity / Hydrolases; Acting on peptide bonds (peptidases); Cysteine endopeptidases / single-stranded RNA binding / regulation of autophagy / viral protein processing / host cell perinuclear region of cytoplasm / lyase activity / host cell endoplasmic reticulum membrane / RNA helicase / symbiont-mediated suppression of host type I interferon-mediated signaling pathway / symbiont-mediated suppression of host gene expression / copper ion binding / viral translational frameshifting / symbiont-mediated activation of host autophagy / RNA-directed RNA polymerase / cysteine-type endopeptidase activity / viral RNA genome replication / RNA-directed RNA polymerase activity / DNA-templated transcription / lipid binding / host cell nucleus / SARS-CoV-2 activates/modulates innate and adaptive immune responses / ATP hydrolysis activity / proteolysis / RNA binding / zinc ion binding / ATP binding / membrane
Similarity search - Function
RNA-dependent RNA polymerase, SARS-CoV-like / Nonstructural protein 14, betacoronavirus / NSP15, NendoU domain, coronavirus / Nonstructural protein 15, middle domain, alpha/betacoronavirus / Nonstructural protein 15, N-terminal domain, alpha/beta-coronavirus / NSP14, guanine-N7-methyltransferase domain, coronavirus / Coronavirus (CoV) guanine-N7-methyltransferase (N7-MTase) domain profile. / Coronavirus (CoV) Nsp15 N-terminal oligomerization domain profile. / NSP12 RNA-dependent RNA polymerase, coronavirus / : ...RNA-dependent RNA polymerase, SARS-CoV-like / Nonstructural protein 14, betacoronavirus / NSP15, NendoU domain, coronavirus / Nonstructural protein 15, middle domain, alpha/betacoronavirus / Nonstructural protein 15, N-terminal domain, alpha/beta-coronavirus / NSP14, guanine-N7-methyltransferase domain, coronavirus / Coronavirus (CoV) guanine-N7-methyltransferase (N7-MTase) domain profile. / Coronavirus (CoV) Nsp15 N-terminal oligomerization domain profile. / NSP12 RNA-dependent RNA polymerase, coronavirus / : / Coronavirus Nsp12 RNA-dependent RNA polymerase (RdRp) domain profile. / Coronavirus Nsp12 Interface domain profile. / : / : / Coronavirus Nonstructural protein 13, 1B domain / Coronavirus Non-structural protein 13, zinc-binding domain / Coronavirus Nonstructural protein 13, stalk domain / Nidovirus 2-O-methyltransferase / Nidovirus 2'-O-methyltransferase (2'-O-MTase) domain profile. / Non-structural protein NSP15, N-terminal domain superfamily, coronavirus / Non-structural protein NSP15, middle domain superfamily / Coronavirus replicase NSP15, N-terminal oligomerization / Nonstructural protein 15, middle domain, coronavirus / Nonstructural protein 13, 1B domain, coronavirus / Coronavirus replicase NSP15, middle domain / Coronavirus replicase NSP15, N-terminal oligomerisation / Nidovirus 3'-5' exoribonuclease domain / Nidovirus 3'-5' exoribonuclease (ExoN) domain profile. / Non-structural protein NSP16, coronavirus-like / Non-structural protein 14, coronavirus / RNA polymerase, N-terminal, coronavirus / Coronavirus 2'-O-methyltransferase / Coronavirus proofreading exoribonuclease / Coronavirus RNA-dependent RNA polymerase, N-terminal / Nonstructural protein 13, zinc-binding domain, coronavirus-like / Coronaviridae zinc-binding (CV ZBD) domain profile. / Arterivirus Nsp11 N-terminal/Coronavirus NSP15 middle domain / Arterivirus Nsp11 N-terminal/coronavirus NSP15 middle (AV-Nsp11N/CoV-Nsp15M) domain profile. / Nidoviral uridylate-specific endoribonuclease (NendoU) domain profile. / Nidovirus RdRp-associated nucleotidyl transferase (NiRAN) domain / Nidovirus RdRp-associated nucleotidyl transferase (NiRAN) domain profile. / Endoribonuclease EndoU-like / NendoU domain, nidovirus / Coronavirus replicase NSP15, uridylate-specific endoribonuclease / : / Lipocalin signature. / DNA2/NAM7 helicase-like, C-terminal / AAA domain / (+) RNA virus helicase core domain / (+)RNA virus helicase core domain profile. / Non-structural protein NSP3, SUD-N (Mac2) domain, betacoronavirus / Sarbecovirus Nsp3c-N domain profile. / Non-structural protein NSP3, N-terminal, betacoronavirus / Polyprotein cleavage domain PL2pro superfamily, betacoronavirus / Non-structural protein NSP3, SUD-N (Mac2) domain superfamily, betacoronavirus / Betacoronavirus SUD-C domain / Betacoronavirus replicase NSP3, N-terminal / NSP1 globular domain superfamily, betacoronavirus / Non-structural protein 2, SARS-CoV-like / Betacoronavirus single-stranded poly(A) binding domain / Betacoronavirus Nsp3c-M domain profile. / NSP1, globular domain, betacoronavirus / Non-structural protein NSP3, SUD-M domain, betacoronavirus / Non-structural protein NSP3, SUD-M domain superfamily, betacoronavirus / NSP1, C-terminal domain, betacoronavirus / Betacoronavirus replicase NSP1 / Betacoronavirus (BetaCoV) Nsp1 C-terminal domain profile. / : / Betacoronavirus Nsp3e group 2-specific marker (G2M) domain profile. / Betacoronavirus Nsp3c-C domain profile. / Betacoronavirus Nsp3e nucleic acid-binding (NAB) domain profile. / DPUP/SUD, C-terminal, betacoronavirus / Non-structural protein NSP3, nucleic acid-binding domain, betacoronavirus / Non-structural protein NSP3A domain-like superfamily / Non-structural protein NSP3, nucleic acid-binding domain superfamily, betacoronavirus / Non-structural protein 6, betacoronavirus / Betacoronavirus nucleic acid-binding (NAB) / Papain-like viral protease, palm and finger domains, coronavirus / Papain-like protease, N-terminal domain superfamily, coronavirus / Carbamoyl-phosphate synthase subdomain signature 2. / Coronavirus replicase NSP2, N-terminal / : / Coronavirus replicase NSP2, C-terminal / Coronavirus (CoV) Nsp2 middle domain profile. / NSP1, globular domain, alpha/betacoronavirus / Coronavirus (CoV) Nsp1 globular domain profile. / Coronavirus (CoV) Nsp2 N-terminal domain profile. / Coronavirus (CoV) Nsp2 C-terminal domain profile. / Nonstructural protein 2, N-terminal domain, coronavirus / Non-structural protein 2, C-terminal domain, coronavirus / NSP3, second ubiquitin-like (Ubl) domain, coronavirus / Coronavirus Nsp3a Ubl domain profile. / Coronavirus Nsp3d Ubl domain profile. / NSP3, first ubiquitin-like (Ubl) domain, coronavirus / Peptidase family C16 domain profile. / : / : / Coronavirus replicase NSP7 / Coronavirus 3Ecto domain profile. / Coronavirus RNA-dependent RNA polymerase (RdRp) Nsp7 cofactor domain profile.
Similarity search - Domain/homology
ACETIC ACID / Replicase polyprotein 1ab
Similarity search - Component
Biological speciesSevere acute respiratory syndrome coronavirus 2
MethodX-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 1.85 Å
AuthorsWilamowski, M. / Kim, Y. / Jedrzejczak, R. / Maltseva, N. / Endres, M. / Godzik, A. / Michalska, K. / Joachimiak, A. / Center for Structural Genomics of Infectious Diseases (CSGID)
Funding support United States, 1items
OrganizationGrant numberCountry
National Institutes of Health/National Institute Of Allergy and Infectious Diseases (NIH/NIAID) United States
CitationJournal: To Be Published
Title: Crystal Structure of the Second Form of the Co-factor Complex of NSP7 and the C-terminal Domain of NSP8 from SARS CoV-2
Authors: Wilamowski, M. / Kim, Y. / Jedrzejczak, R. / Maltseva, N. / Endres, M. / Godzik, A. / Michalska, K. / Joachimiak, A. / Center for Structural Genomics of Infectious Diseases (CSGID)
History
DepositionMay 2, 2020Deposition site: RCSB / Processing site: RCSB
Revision 1.0May 13, 2020Provider: repository / Type: Initial release
Revision 1.1Jan 27, 2021Group: Structure summary / Category: entity / entity_name_com / Item: _entity.pdbx_description
Revision 1.2Oct 18, 2023Group: Data collection / Database references / Refinement description
Category: chem_comp_atom / chem_comp_bond ...chem_comp_atom / chem_comp_bond / database_2 / pdbx_initial_refinement_model
Item: _database_2.pdbx_DOI / _database_2.pdbx_database_accession
Revision 1.3Oct 23, 2024Group: Structure summary / Category: pdbx_entry_details / pdbx_modification_feature / Item: _pdbx_entry_details.has_protein_modification

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
A: Non-structural protein 7
B: Non-structural protein 8
C: Non-structural protein 7
D: Non-structural protein 8
hetero molecules


Theoretical massNumber of molelcules
Total (without water)46,1936
Polymers46,0734
Non-polymers1202
Water1,44180
1
A: Non-structural protein 7
B: Non-structural protein 8
hetero molecules

C: Non-structural protein 7
D: Non-structural protein 8
hetero molecules


Theoretical massNumber of molelcules
Total (without water)46,1936
Polymers46,0734
Non-polymers1202
Water724
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
crystal symmetry operation1_556x,y,z+11
Buried area7640 Å2
ΔGint-80 kcal/mol
Surface area18000 Å2
MethodPISA
Unit cell
Length a, b, c (Å)35.590, 120.081, 43.309
Angle α, β, γ (deg.)90.000, 91.953, 90.000
Int Tables number4
Space group name H-MP1211
Space group name HallP2yb
Symmetry operation#1: x,y,z
#2: -x,y+1/2,-z

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Components

#1: Protein Non-structural protein 7 / nsp7 / SARS-CoV-2 NSP7


Mass: 9521.062 Da / Num. of mol.: 2 / Fragment: UNP residues 3860-3942
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Severe acute respiratory syndrome coronavirus 2
Gene: rep, 1a-1b / Plasmid: pMCSG53 / Production host: Escherichia coli BL21(DE3) (bacteria) / Variant (production host): Gold / References: UniProt: P0DTD1
#2: Protein Non-structural protein 8 / nsp8 / SARS-CoV-2 NSP8


Mass: 13515.529 Da / Num. of mol.: 2 / Fragment: C-terminal domain (UNP residues 4019-4140)
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Severe acute respiratory syndrome coronavirus 2
Gene: rep, 1a-1b / Plasmid: pMCSG120 / Production host: Escherichia coli BL21(DE3) (bacteria) / Variant (production host): Gold / References: UniProt: P0DTD1
#3: Chemical ChemComp-ACY / ACETIC ACID


Mass: 60.052 Da / Num. of mol.: 2 / Source method: obtained synthetically / Formula: C2H4O2
#4: Water ChemComp-HOH / water


Mass: 18.015 Da / Num. of mol.: 80 / Source method: isolated from a natural source / Formula: H2O
Has ligand of interestN
Has protein modificationY

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Experimental details

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Experiment

ExperimentMethod: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

CrystalDensity Matthews: 2.01 Å3/Da / Density % sol: 38.73 %
Crystal growTemperature: 289 K / Method: vapor diffusion, sitting drop / pH: 5.5
Details: 0.2 M ammonium acetate, 0.1 M Bis-Tris, pH 5.5, 25% w/v PEG3350

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Data collection

DiffractionMean temperature: 100 K / Serial crystal experiment: N
Diffraction sourceSource: SYNCHROTRON / Site: APS / Beamline: 19-ID / Wavelength: 0.97918 Å
DetectorType: DECTRIS PILATUS3 X 6M / Detector: PIXEL / Date: Apr 28, 2020
RadiationMonochromator: double crystal Si(111) / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelengthWavelength: 0.97918 Å / Relative weight: 1
ReflectionResolution: 1.85→50 Å / Num. obs: 56822 / % possible obs: 93 % / Redundancy: 1.8 % / Biso Wilson estimate: 37.91 Å2 / CC1/2: 0.967 / Rmerge(I) obs: 0.083 / Net I/σ(I): 12.9
Reflection shellResolution: 1.85→1.88 Å / Redundancy: 1.4 % / Rmerge(I) obs: 0.835 / Mean I/σ(I) obs: 0.75 / Num. unique obs: 2429 / CC1/2: 0.338 / % possible all: 78.9

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Processing

Software
NameVersionClassification
PHENIX1.17.1_3660refinement
HKL-3000data reduction
HKL-3000data scaling
HKL-3000phasing
MOLREPphasing
RefinementMethod to determine structure: MOLECULAR REPLACEMENT
Starting model: PDB entry 6WIQ

6wiq


Resolution: 1.85→35.57 Å / SU ML: 0.2566 / Cross valid method: FREE R-VALUE / σ(F): 1.36 / Phase error: 26.1026
Stereochemistry target values: GeoStd + Monomer Library + CDL v1.2
RfactorNum. reflection% reflection
Rfree0.2141 1461 4.88 %
Rwork0.1868 28470 -
obs0.1881 29931 96.83 %
Solvent computationShrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL
Displacement parametersBiso mean: 49.91 Å2
Refinement stepCycle: LAST / Resolution: 1.85→35.57 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms2902 0 8 80 2990
Refine LS restraints
Refine-IDTypeDev idealNumber
X-RAY DIFFRACTIONf_bond_d0.00262974
X-RAY DIFFRACTIONf_angle_d0.48684038
X-RAY DIFFRACTIONf_chiral_restr0.0406513
X-RAY DIFFRACTIONf_plane_restr0.0031502
X-RAY DIFFRACTIONf_dihedral_angle_d16.49681094
LS refinement shell
Resolution (Å)Rfactor RfreeNum. reflection RfreeRfactor RworkNum. reflection RworkRefine-ID% reflection obs (%)
1.85-1.920.34541130.31322593X-RAY DIFFRACTION88.46
1.92-1.990.31051500.27452779X-RAY DIFFRACTION94.73
1.99-2.080.2651490.25282814X-RAY DIFFRACTION96.05
2.08-2.190.24381670.22112882X-RAY DIFFRACTION98.87
2.19-2.330.26381410.21222886X-RAY DIFFRACTION98.44
2.33-2.510.23581290.21442888X-RAY DIFFRACTION97.13
2.51-2.760.23941510.20552906X-RAY DIFFRACTION99.25
2.76-3.160.22981770.20482884X-RAY DIFFRACTION98.81
3.16-3.980.20131400.17762918X-RAY DIFFRACTION98.42
3.99-35.570.17191440.14882920X-RAY DIFFRACTION98.11
Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

IDL11 (°2)L12 (°2)L13 (°2)L22 (°2)L23 (°2)L33 (°2)S11 (Å °)S12 (Å °)S13 (Å °)S21 (Å °)S22 (Å °)S23 (Å °)S31 (Å °)S32 (Å °)S33 (Å °)T11 (Å2)T12 (Å2)T13 (Å2)T22 (Å2)T23 (Å2)T33 (Å2)Origin x (Å)Origin y (Å)Origin z (Å)
16.316926187682.690542491413.827078778456.514476426591.082835667392.55130754265-0.01422404658250.4946472423440.2242039261060.0494571740860.191227427687-0.8233605749370.6725695848631.51305412631-0.1372137046960.3115947705880.06843038244450.02266371329470.406900290731-0.07652667672150.40483419760919.522921071311.86681038356.16083278813
25.738525548231.55154418134.070934594816.644770508143.484824931729.11286723344-0.0617808497869-0.04334122887220.358309890126-0.0744712424214-0.03140974761180.0996470774026-0.08869876247210.2630424864090.06505937600110.2860393784810.02867741318680.007349533635110.22170736069-0.03062349345150.30138222622416.178133752721.650321228315.2268553035
32.80315872430.7049411845771.95704012523.865508200912.156942956245.913532633720.419212551829-0.67610490147-0.101611679470.484097363012-0.0741558525651-0.6549749356761.053914799760.580526905477-0.3365344125780.6162557860370.0691679694427-0.1807436559960.413966965725-0.0288027040090.48434753561523.474810009321.97346440828.8306105979
44.49594055782-0.3337214809974.336706204488.929099586432.882956661684.97959321859-0.0735020816874-0.3437119987070.266130721729-0.005416975532230.02567357169910.1501817529050.00845230846209-0.4253279671340.2284318262810.3691536151980.0238706322848-0.04940768070280.303731798092-0.06836684246960.41465525666712.600003164324.835736860615.5033614807
55.53911193747-0.3690775708846.015719666725.19732685310.5921463866938.63664500058-0.1284779157250.5911209844520.4220449129120.06494166510570.0592864031172-0.455766554221-0.03019008904080.5504381466620.1118274306510.373215779006-0.03111326278950.04120616546090.273538371647-0.01150114075080.289686525233.95927063721-3.9861256537238.0196121644
63.83587475742-1.533469100772.777664906083.158658469570.4929139685264.02589108785-0.393618128341-1.00541070812-0.1767865503491.642339478591.927929019723.71344337574-0.608753463245-2.95046866417-1.132000741750.8532777548540.1940444926320.1300618092751.0242172720.08359426774920.68580126528-12.9147067716-8.0082597146641.3646758385
72.530268905693.908975312340.3994863777946.394458850680.4121704686969.416771135150.07578040182640.348069505846-0.203873948812-0.3485896555170.0227655143551-0.1617174979170.538523011930.253317178016-0.1196848921970.427057372470.0334445918761-0.003463043487360.2662986679880.009665690932380.2686756850591.74343529306-13.539505190841.8515310169
82.765246386280.2322953736934.753064887074.3605006368-0.07332728707019.463961626990.5491981438840.236772269581-0.396419844619-0.364099069624-0.150168183772-0.1865571329630.8638125161450.435776057061-0.3556452146560.5586234034240.04655914117270.04732685931740.323945126259-0.02101564954050.3333267166493.67960682068-12.648437081632.9022608489
98.842063920993.4122405508-5.980947645614.34725625339-0.988698763185.721541313881.530118689771.22637823890.981922554297-0.8564346517051.0089391133.72953765471.24859663005-0.157546098856-2.474915037150.652983563244-0.018865472374-0.1030545859440.7175868996410.1308032590610.953237918567-10.9661495872-5.576256115927.6859261659
103.813941455173.778543458214.148604898294.540308043974.234907621854.62412920983-0.05141009918881.38476314469-0.250397329486-2.932714691630.3580709733440.0153811127228-1.44879599355-0.567391340723-0.2829660221080.782795185515-0.02279528814680.08015643390670.5149030232610.08956254588120.545939648339-3.686517219121.8822013028122.4406349114
114.58467080277-6.69836864977-6.108765048378.937317938258.018941269059.01136421920.1441824268280.293842160720.177544502868-0.258754396431-0.18682298731-0.153370238555-0.147680071361-0.260590595794-0.0147629409140.295732228714-0.03203716699030.0281919127520.3610523677660.0187693562680.280402728207-0.8828562290813.5960067235932.2434827173
124.966153129843.179919418293.250255620967.303156043031.156702442814.94367419820.430435917418-0.222055529027-0.3373186244180.253237339355-0.05219829855870.09294838553760.376845221906-0.097837773249-0.3199886430430.341915897618-0.004951220754560.01643119471640.27119209040.01098498905180.235613870848-2.00980204101-16.051277379224.7692641609
138.467609709980.08055845241723.078453661292.695946483140.7863548684824.507639328710.2308029411040.579148143743-0.546149400432-0.2989100307540.185396155089-0.1111957134220.3206388572170.342879688349-0.3893852890790.424077220106-0.0459731487575-0.02425813221630.328416253914-0.0005396983383420.377606705036-6.52035232552-22.755468642614.9520917671
147.806977918451.46121032211.077935611236.87692915163-2.436329715024.31462506345-0.1640116662970.4007240857730.0334753430493-0.07238056634480.362928188774-0.06770950905610.567786326024-0.112507343806-0.2792720587890.2609090711820.01031314417270.01096306467540.214167477637-0.03725150169370.2834623458814.591135534663.608536906142.43128262317
157.596645291660.1190032986852.49515862186.0664334288-2.440726385735.62245065604-0.1382675521860.206044037270.7810086952540.1488686700870.1497154058550.240147847561-0.587232042463-0.1347932603080.002477798857870.3384813120040.01830982906880.03355838628430.250511731837-0.01816828604690.3458373763343.4243951306712.45664965722.3626714537
163.79285085463-5.484844426780.9858858786968.14840164927-2.623646697233.008980370430.556922941048-0.655221711773-0.5946747927640.848279154333-0.366201110856-0.6195405226260.5613516438820.985355990618-0.2595906803040.8944175846270.0627006347016-0.4065184419690.833035635816-0.05805522372150.71673245499415.09973391972.2661425165413.9501802818
177.691365907045.71709781324-3.305198133955.91243255041-1.215185182673.97513202437-0.0702996907789-0.210787965471-0.550532346381-0.629880133038-0.287399828445-0.8008839315150.8327639574680.4608011642770.3367318844270.3284251187460.05871289027170.0008919792223420.340872262452-0.03152797682930.2955372332212.2193984382-3.331735380494.30294015123
Refinement TLS group
IDRefine-IDRefine TLS-IDSelection details
1X-RAY DIFFRACTION1chain 'D' and (resid 99 through 112 )
2X-RAY DIFFRACTION2chain 'D' and (resid 113 through 160 )
3X-RAY DIFFRACTION3chain 'D' and (resid 161 through 183 )
4X-RAY DIFFRACTION4chain 'D' and (resid 184 through 192 )
5X-RAY DIFFRACTION5chain 'A' and (resid 1 through 19 )
6X-RAY DIFFRACTION6chain 'A' and (resid 20 through 26 )
7X-RAY DIFFRACTION7chain 'A' and (resid 27 through 44 )
8X-RAY DIFFRACTION8chain 'A' and (resid 45 through 60 )
9X-RAY DIFFRACTION9chain 'A' and (resid 61 through 67 )
10X-RAY DIFFRACTION10chain 'A' and (resid 68 through 73 )
11X-RAY DIFFRACTION11chain 'B' and (resid 79 through 98 )
12X-RAY DIFFRACTION12chain 'B' and (resid 99 through 134 )
13X-RAY DIFFRACTION13chain 'B' and (resid 135 through 194 )
14X-RAY DIFFRACTION14chain 'C' and (resid 1 through 25 )
15X-RAY DIFFRACTION15chain 'C' and (resid 26 through 60 )
16X-RAY DIFFRACTION16chain 'C' and (resid 61 through 72 )
17X-RAY DIFFRACTION17chain 'D' and (resid 78 through 98 )

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