CIRCADIAN CLOCK PROTEIN / Flavoprotein / Sortylation Linker
Function / homology
Function and homology information
UV-A, blue light phototransduction / magnetoreception / detection of light stimulus involved in magnetoreception / gravitaxis / Phosphorylation of PER and TIM / Degradation of CRY / Degradation of TIM / blue light signaling pathway / response to magnetism / response to blue light ...UV-A, blue light phototransduction / magnetoreception / detection of light stimulus involved in magnetoreception / gravitaxis / Phosphorylation of PER and TIM / Degradation of CRY / Degradation of TIM / blue light signaling pathway / response to magnetism / response to blue light / regulation of circadian sleep/wake cycle, sleep / cellular response to light stimulus / blue light photoreceptor activity / entrainment of circadian clock / circadian behavior / entrainment of circadian clock by photoperiod / locomotor rhythm / photoreceptor activity / phototransduction / response to light stimulus / FAD binding / circadian regulation of gene expression / regulation of circadian rhythm / circadian rhythm / flavin adenine dinucleotide binding / negative regulation of DNA-templated transcription / perinuclear region of cytoplasm / DNA binding / nucleoplasm / nucleus / cytoplasm / cytosol Similarity search - Function
Cryptochrome/DNA photolyase class 1 / Cryptochrome/DNA photolyase, FAD-binding domain / FAD binding domain of DNA photolyase / DNA photolyase, N-terminal / Cryptochrome/photolyase, N-terminal domain superfamily / DNA photolyase / Photolyase/cryptochrome alpha/beta domain profile. / Cryptochrome/DNA photolyase, FAD-binding domain-like superfamily / Rossmann-like alpha/beta/alpha sandwich fold Similarity search - Domain/homology
Evidence: mass spectrometry, Liquid chromatography and tandem mass spectrometry (LC-MS/MS) determined fragmentation spectra for the QFFWLADLPGTGGGGC fragment of the sortylated Drosophila cryptochrome.
Type
Name
Symmetry operation
Number
identity operation
1_555
x,y,z
1
Buried area
4920 Å2
ΔGint
-47 kcal/mol
Surface area
43670 Å2
Method
PISA
Unit cell
Length a, b, c (Å)
72.979, 122.186, 81.954
Angle α, β, γ (deg.)
90.000, 115.480, 90.000
Int Tables number
4
Space group name H-M
P1211
Space group name Hall
P2yb
Symmetry operation
#1: x,y,z #2: -x,y+1/2,-z
-
Components
#1: Protein
Cryptochrome-1 / dcry / Blue light photoreceptor
Mass: 67602.773 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Drosophila melanogaster (fruit fly) / Gene: cry, CG3772 / Plasmid: pET28a(+) / Production host: Escherichia coli (E. coli) / Strain (production host): CmpX13 / References: UniProt: O77059
Mass: 18.015 Da / Num. of mol.: 95 / Source method: isolated from a natural source / Formula: H2O
Has ligand of interest
Y
Sequence details
The authors state that the cryptochrome has a LPGTG motif encoded on the C-terminus followed by a ...The authors state that the cryptochrome has a LPGTG motif encoded on the C-terminus followed by a GGGGC peptide covalently attached by the enzyme Sortase A. The LPGTG is a molecular recognition site for Sortase A, and the donor peptide is the GGGGC.
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Experimental details
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Experiment
Experiment
Method: X-RAY DIFFRACTION / Number of used crystals: 1
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Sample preparation
Crystal
Density Matthews: 2.44 Å3/Da / Density % sol: 50.8 % / Description: Plate-like crystals with slight curvature.
Crystal grow
Temperature: 298 K / Method: vapor diffusion, hanging drop / pH: 9 Details: 4 microliter drop (2 microliter protein solution, 2 microliter well solution) 100 mM Tris (pH 9) 150 mM Magneisum Acetate Tetrahydrate 17% PEG-4000 8 mg/mL sort-tagged WT Drosophila Cryptochrome Temp details: Room Temperature
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Data collection
Diffraction
Mean temperature: 100 K / Serial crystal experiment: N
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