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Open data
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Basic information
Entry | Database: PDB / ID: 6wsh | ||||||
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Title | Crystal structure of EutV from Enterococcus faecalis | ||||||
![]() | Response regulator | ||||||
![]() | RNA BINDING PROTEIN / RNA binding / ANTAR domain / antitermination | ||||||
Function / homology | ![]() | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Ataide, S.F. / Walshe, J.L. | ||||||
![]() | ![]() Title: Structural characterization of the ANTAR antiterminator domain bound to RNA. Authors: Walshe, J.L. / Siddiquee, R. / Patel, K. / Ataide, S.F. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 163.5 KB | Display | ![]() |
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PDB format | ![]() | 128 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
Related structure data | ![]() 6ww6C ![]() 1qo0S ![]() 1s8nS S: Starting model for refinement C: citing same article ( |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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1 |
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Unit cell |
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Components
#1: Protein | Mass: 21529.006 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() Gene: BZG32_08120, CUN08_06285, DVW78_01210, FKY84_00940, KUB3007_C13700 Production host: ![]() ![]() #2: Chemical | #3: Chemical | ChemComp-NA / #4: Chemical | ChemComp-GOL / | #5: Water | ChemComp-HOH / | Has ligand of interest | N | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.64 Å3/Da / Density % sol: 53.49 % |
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Crystal grow | Temperature: 291.15 K / Method: vapor diffusion, sitting drop / pH: 8.5 Details: 0.075M Tris pH 8.5, 18-20% v/v tert-butanol, 25% v/v glycerol |
-Data collection
Diffraction | Mean temperature: 80 K / Serial crystal experiment: N |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: DECTRIS EIGER X 16M / Detector: PIXEL / Date: Mar 4, 2017 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.9537 Å / Relative weight: 1 |
Reflection | Resolution: 2.12→42.85 Å / Num. obs: 26642 / % possible obs: 99.3 % / Redundancy: 4.9 % / Biso Wilson estimate: 15.57 Å2 / CC1/2: 0.984 / Net I/σ(I): 6 |
Reflection shell | Resolution: 2.12→2.18 Å / Redundancy: 2.9 % / Num. unique obs: 2134 / CC1/2: 0.377 / % possible all: 100 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: 1s8n, 1qo0 Resolution: 2.12→42.84 Å / SU ML: 0.2558 / Cross valid method: FREE R-VALUE / σ(F): 1.34 / Phase error: 22.0912 Stereochemistry target values: GeoStd + Monomer Library + CDL v1.2
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 22.97 Å2 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.12→42.84 Å
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Refine LS restraints |
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LS refinement shell |
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