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- PDB-6ws3: The crystal structure of the 2009/H1N1/California PA endonuclease... -
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Open data
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Basic information
Entry | Database: PDB / ID: 6ws3 | ||||||
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Title | The crystal structure of the 2009/H1N1/California PA endonuclease wild type bound to DNA oligomers TG and AGCA (from cleaved GTGAGCAGTG) | ||||||
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![]() | VIRAL PROTEIN/DNA / NUCLEASE / INFLUENZA / DNA oligomer / cleaved / VIRAL PROTEIN / VIRAL PROTEIN-DNA complex / DNA BINDING PROTEIN | ||||||
Function / homology | ![]() | ||||||
Biological species | ![]() ![]() synthetic construct (others) | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Cuypers, M.G. / Kumar, G. / Webb, T. / White, S.W. | ||||||
![]() | ![]() Title: Structural insights into the substrate specificity of the endonuclease activity of the influenza virus cap-snatching mechanism. Authors: Kumar, G. / Cuypers, M. / Webby, R.R. / Webb, T.R. / White, S.W. | ||||||
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 101.7 KB | Display | ![]() |
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PDB format | ![]() | 74.6 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 819 KB | Display | ![]() |
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Full document | ![]() | 819.6 KB | Display | |
Data in XML | ![]() | 10.1 KB | Display | |
Data in CIF | ![]() | 13.3 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 6w7aC ![]() 6whmC ![]() 7kafC ![]() 7kbcC ![]() 7kl3C ![]() 5vptS C: citing same article ( S: Starting model for refinement |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
-Protein / DNA chain , 2 types, 3 molecules AFE
#1: Protein | Mass: 23148.344 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() Gene: PA-X, PA / Strain: A/Luxembourg/43/2009(H1N1) / Production host: ![]() ![]() |
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#2: DNA chain | Mass: 3125.052 Da / Num. of mol.: 2 / Source method: obtained synthetically / Details: cleaved by PAN09 between G3 and A4 / Source: (synth.) synthetic construct (others) |
-Non-polymers , 4 types, 82 molecules ![](data/chem/img/GOL.gif)
![](data/chem/img/MN.gif)
![](data/chem/img/SO4.gif)
![](data/chem/img/HOH.gif)
![](data/chem/img/MN.gif)
![](data/chem/img/SO4.gif)
![](data/chem/img/HOH.gif)
#3: Chemical | #4: Chemical | ChemComp-MN / | #5: Chemical | #6: Water | ChemComp-HOH / | |
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-Details
Has ligand of interest | Y |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.29 Å3/Da / Density % sol: 46.18 % |
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Crystal grow | Temperature: 291 K / Method: vapor diffusion, hanging drop Details: 0.1 M HEPES PH 7.8, 1 M AMMONIUM SULFATE, 0.02M MGCL2, 0.02M MNCL2 |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: RAYONIX MX300-HS / Detector: CCD / Date: Jun 7, 2016 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1 Å / Relative weight: 1 |
Reflection | Resolution: 2.2→50 Å / Num. obs: 14021 / % possible obs: 99 % / Redundancy: 3.2 % / CC1/2: 0.996 / Rmerge(I) obs: 0.078 / Rpim(I) all: 0.042 / Rrim(I) all: 0.104 / Net I/σ(I): 20.8 |
Reflection shell | Resolution: 2.2→2.28 Å / Redundancy: 1.9 % / Rmerge(I) obs: 0.79 / Mean I/σ(I) obs: 1.4 / Num. unique obs: 2145 / CC1/2: 0.684 / Rpim(I) all: 0.486 / Rrim(I) all: 0.969 / % possible all: 92.1 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: 5vpt Resolution: 2.2→38.4 Å / SU ML: 0.28 / Cross valid method: THROUGHOUT / σ(F): 1.34 / Phase error: 29.17
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso max: 221.2 Å2 / Biso mean: 77.7445 Å2 / Biso min: 37.24 Å2 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: final / Resolution: 2.2→38.4 Å
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LS refinement shell | Refine-ID: X-RAY DIFFRACTION / Rfactor Rfree error: 0 / Total num. of bins used: 5
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Refinement TLS params. | Method: refined / Refine-ID: X-RAY DIFFRACTION
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Refinement TLS group |
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