[English] 日本語
Yorodumi- PDB-6whm: The crystal structure of the 2009/H1N1/California PA endonuclease... -
+Open data
-Basic information
Entry | Database: PDB / ID: 6whm | ||||||
---|---|---|---|---|---|---|---|
Title | The crystal structure of the 2009/H1N1/California PA endonuclease wild type bound to DNA oligomer TAGC (cleaved TTAGCATT, 5mM overnight DNA soak) | ||||||
Components |
| ||||||
Keywords | VIRAL PROTEIN/DNA / NUCLEASE / INFLUENZA / DNA oligomer / cleaved / VIRAL PROTEIN / VIRAL PROTEIN-DNA complex / DNA BINDING PROTEIN | ||||||
Function / homology | Function and homology information | ||||||
Biological species | Influenza A virus synthetic construct (others) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 1.92 Å | ||||||
Authors | Cuypers, M.G. / Kumar, G. / Webb, T. / White, S.W. | ||||||
Citation | Journal: Nucleic Acids Res. / Year: 2021 Title: Structural insights into the substrate specificity of the endonuclease activity of the influenza virus cap-snatching mechanism. Authors: Kumar, G. / Cuypers, M. / Webby, R.R. / Webb, T.R. / White, S.W. | ||||||
History |
|
-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
---|
-Downloads & links
-Download
PDBx/mmCIF format | 6whm.cif.gz | 103.3 KB | Display | PDBx/mmCIF format |
---|---|---|---|---|
PDB format | pdb6whm.ent.gz | 75.1 KB | Display | PDB format |
PDBx/mmJSON format | 6whm.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 6whm_validation.pdf.gz | 462.4 KB | Display | wwPDB validaton report |
---|---|---|---|---|
Full document | 6whm_full_validation.pdf.gz | 463.6 KB | Display | |
Data in XML | 6whm_validation.xml.gz | 10.8 KB | Display | |
Data in CIF | 6whm_validation.cif.gz | 14.6 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/wh/6whm ftp://data.pdbj.org/pub/pdb/validation_reports/wh/6whm | HTTPS FTP |
-Related structure data
Related structure data | 6w7aC 6ws3C 7kafC 7kbcC 7kl3C 5vptS S: Starting model for refinement C: citing same article (ref.) |
---|---|
Similar structure data |
-Links
-Assembly
Deposited unit |
| ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
1 |
| ||||||||||
Unit cell |
| ||||||||||
Components on special symmetry positions |
|
-Components
-Protein / DNA chain , 2 types, 2 molecules AB
#1: Protein | Mass: 23148.344 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Influenza A virus, (gene. exp.) Influenza A virus (A/Luxembourg/43/2009(H1N1)) Cell line: 2009/H1N1/CALIFORNIA / Gene: PA-X, PA / Variant: 2009/H1N1/CALIFORNIA / Strain: A/Luxembourg/43/2009(H1N1) / Production host: Escherichia coli BL21(DE3) (bacteria) / References: UniProt: A0A481PFF5, UniProt: C6H0Y9 |
---|---|
#2: DNA chain | Mass: 2416.615 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) synthetic construct (others) / Production host: synthetic construct (others) |
-Non-polymers , 5 types, 143 molecules
#3: Chemical | #4: Chemical | #5: Chemical | ChemComp-MN / | #6: Chemical | #7: Water | ChemComp-HOH / | |
---|
-Details
Has ligand of interest | N |
---|
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
---|
-Sample preparation
Crystal | Density Matthews: 2.65 Å3/Da / Density % sol: 53.63 % |
---|---|
Crystal grow | Temperature: 291 K / Method: vapor diffusion, hanging drop Details: 0.1 M HEPES PH 7.8, 1 M AMMONIUM SULFATE, 0.02M MGCL2, 0.02M MNCL2. Temp details: controlled Temperature room |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N |
---|---|
Diffraction source | Source: SYNCHROTRON / Site: APS / Beamline: 22-ID / Wavelength: 1 Å |
Detector | Type: RAYONIX MX300-HS / Detector: CCD / Date: Aug 9, 2016 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1 Å / Relative weight: 1 |
Reflection | Resolution: 1.92→50 Å / Num. obs: 21245 / % possible obs: 99.4 % / Redundancy: 7.3 % / Biso Wilson estimate: 39.39 Å2 / CC1/2: 0.999 / Rmerge(I) obs: 0.049 / Rpim(I) all: 0.023 / Rrim(I) all: 0.061 / Net I/σ(I): 46.4 |
Reflection shell | Resolution: 1.92→1.99 Å / Rmerge(I) obs: 0.779 / Mean I/σ(I) obs: 2 / Num. unique obs: 2048 / CC1/2: 0.786 / Rpim(I) all: 0.308 / Rrim(I) all: 0.74 / % possible all: 95.5 |
-Processing
Software |
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: 5vpt Resolution: 1.92→37.35 Å / SU ML: 0.23 / Cross valid method: THROUGHOUT / σ(F): 1.35 / Phase error: 23.27 / Stereochemistry target values: ML
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso max: 184.86 Å2 / Biso mean: 59.2088 Å2 / Biso min: 28.91 Å2 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: final / Resolution: 1.92→37.35 Å
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
LS refinement shell | Refine-ID: X-RAY DIFFRACTION / Rfactor Rfree error: 0 / Total num. of bins used: 7
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement TLS params. | Method: refined / Refine-ID: X-RAY DIFFRACTION
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement TLS group |
|