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- PDB-6wq2: Cryo-EM of the S. islandicus filamentous virus, SIFV -

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Basic information

Entry
Database: PDB / ID: 6wq2
TitleCryo-EM of the S. islandicus filamentous virus, SIFV
Components
  • (A-DNA) x 2
  • Structural protein MCP1
  • Structural protein MCP2
KeywordsVIRUS / helical symmetry / archaeal virus / rod-like virus / structural protein
Function / homologyhelical viral capsid / DNA binding / DNA / DNA (> 10) / DNA (> 100) / Major capsid protein 1 / Major capsid protein 2
Function and homology information
Biological speciesSulfolobus islandicus filamentous virus
MethodELECTRON MICROSCOPY / helical reconstruction / cryo EM / Resolution: 4 Å
AuthorsWang, F. / Baquero, D.P. / Su, Z. / Zheng, W. / Prangishvili, D. / Krupovic, M. / Egelman, E.H.
Funding support United States, 1items
OrganizationGrant numberCountry
National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)R35GM122510 United States
CitationJournal: Proc Natl Acad Sci U S A / Year: 2020
Title: Structures of filamentous viruses infecting hyperthermophilic archaea explain DNA stabilization in extreme environments.
Authors: Fengbin Wang / Diana P Baquero / Leticia C Beltran / Zhangli Su / Tomasz Osinski / Weili Zheng / David Prangishvili / Mart Krupovic / Edward H Egelman /
Abstract: Living organisms expend metabolic energy to repair and maintain their genomes, while viruses protect their genetic material by completely passive means. We have used cryo-electron microscopy (cryo-EM) ...Living organisms expend metabolic energy to repair and maintain their genomes, while viruses protect their genetic material by completely passive means. We have used cryo-electron microscopy (cryo-EM) to solve the atomic structures of two filamentous double-stranded DNA viruses that infect archaeal hosts living in nearly boiling acid: rod-shaped virus 1 (SSRV1), at 2.8-Å resolution, and filamentous virus (SIFV), at 4.0-Å resolution. The SIFV nucleocapsid is formed by a heterodimer of two homologous proteins and is membrane enveloped, while SSRV1 has a nucleocapsid formed by a homodimer and is not enveloped. In both, the capsid proteins wrap around the DNA and maintain it in an A-form. We suggest that the A-form is due to both a nonspecific desolvation of the DNA by the protein, and a specific coordination of the DNA phosphate groups by positively charged residues. We extend these observations by comparisons with four other archaeal filamentous viruses whose structures we have previously determined, and show that all 10 capsid proteins (from four heterodimers and two homodimers) have obvious structural homology while sequence similarity can be nonexistent. This arises from most capsid residues not being under any strong selective pressure. The inability to detect homology at the sequence level arises from the sampling of viruses in this part of the biosphere being extremely sparse. Comparative structural and genomic analyses suggest that nonenveloped archaeal viruses have evolved from enveloped viruses by shedding the membrane, indicating that this trait may be relatively easily lost during virus evolution.
History
DepositionApr 28, 2020Deposition site: RCSB / Processing site: RCSB
Revision 1.0Jul 29, 2020Provider: repository / Type: Initial release
Revision 1.1Oct 6, 2021Group: Database references / Category: citation / citation_author / database_2
Item: _citation.journal_volume / _citation.page_first ..._citation.journal_volume / _citation.page_first / _citation.page_last / _citation.pdbx_database_id_PubMed / _citation.title / _database_2.pdbx_DOI / _database_2.pdbx_database_accession
Revision 1.2May 29, 2024Group: Data collection / Category: chem_comp_atom / chem_comp_bond

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Assembly

Deposited unit
1: A-DNA
2: A-DNA
A: Structural protein MCP2
B: Structural protein MCP2
C: Structural protein MCP2
D: Structural protein MCP2
E: Structural protein MCP2
F: Structural protein MCP2
G: Structural protein MCP2
H: Structural protein MCP2
I: Structural protein MCP2
J: Structural protein MCP2
K: Structural protein MCP2
L: Structural protein MCP2
M: Structural protein MCP2
N: Structural protein MCP2
P: Structural protein MCP2
R: Structural protein MCP2
T: Structural protein MCP2
a: Structural protein MCP1
b: Structural protein MCP1
c: Structural protein MCP1
d: Structural protein MCP1
e: Structural protein MCP1
f: Structural protein MCP1
g: Structural protein MCP1
h: Structural protein MCP1
i: Structural protein MCP1
j: Structural protein MCP1
k: Structural protein MCP1
l: Structural protein MCP1
m: Structural protein MCP1
n: Structural protein MCP1
p: Structural protein MCP1
r: Structural protein MCP1
t: Structural protein MCP1


Theoretical massNumber of molelcules
Total (without water)843,02336
Polymers843,02336
Non-polymers00
Water00
1


  • Idetical with deposited unit
  • defined by author
  • Evidence: microscopy, helical filament was observed by negative staining and Cryo-EM
TypeNameSymmetry operationNumber
identity operation1_5551
Buried area252950 Å2
ΔGint-1727 kcal/mol
Surface area234640 Å2

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Components

#1: DNA chain A-DNA


Mass: 69416.977 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Sulfolobus islandicus filamentous virus
#2: DNA chain A-DNA


Mass: 68790.570 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Sulfolobus islandicus filamentous virus
#3: Protein
Structural protein MCP2


Mass: 18901.863 Da / Num. of mol.: 17 / Source method: isolated from a natural source / Source: (natural) Sulfolobus islandicus filamentous virus / References: UniProt: Q914J5
#4: Protein
Structural protein MCP1


Mass: 22557.898 Da / Num. of mol.: 17 / Source method: isolated from a natural source / Source: (natural) Sulfolobus islandicus filamentous virus / References: UniProt: Q914J4

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Experimental details

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Experiment

ExperimentMethod: ELECTRON MICROSCOPY
EM experimentAggregation state: FILAMENT / 3D reconstruction method: helical reconstruction

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Sample preparation

ComponentName: Sulfolobus islandicus filamentous virus / Type: VIRUS / Entity ID: all / Source: NATURAL
Source (natural)Organism: Sulfolobus islandicus filamentous virus
Details of virusEmpty: NO / Enveloped: YES / Isolate: STRAIN / Type: VIRION
Natural hostOrganism: Sulfolobus islandicus
Buffer solutionpH: 6
SpecimenEmbedding applied: NO / Shadowing applied: NO / Staining applied: NO / Vitrification applied: YES
Specimen supportDetails: unspecified
VitrificationCryogen name: ETHANE

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Electron microscopy imaging

Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company
MicroscopyModel: FEI TITAN KRIOS
Electron gunElectron source: FIELD EMISSION GUN / Accelerating voltage: 300 kV / Illumination mode: FLOOD BEAM
Electron lensMode: BRIGHT FIELD
Image recordingElectron dose: 44 e/Å2 / Film or detector model: FEI FALCON III (4k x 4k)

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Processing

SoftwareName: PHENIX / Version: 1.16_3549: / Classification: refinement
CTF correctionType: PHASE FLIPPING AND AMPLITUDE CORRECTION
Helical symmertyAngular rotation/subunit: 38.49 ° / Axial rise/subunit: 5.48 Å / Axial symmetry: C1
3D reconstructionResolution: 4 Å / Resolution method: OTHER / Num. of particles: 167500 / Symmetry type: HELICAL
Refine LS restraints
Refine-IDTypeDev idealNumber
ELECTRON MICROSCOPYf_bond_d0.00558327
ELECTRON MICROSCOPYf_angle_d0.67681125
ELECTRON MICROSCOPYf_dihedral_angle_d9.65933119
ELECTRON MICROSCOPYf_chiral_restr0.0399340
ELECTRON MICROSCOPYf_plane_restr0.0058642

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