+Open data
-Basic information
Entry | Database: PDB / ID: 6vs1 | ||||||
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Title | protein C | ||||||
Components | Multidrug transporter MdfA | ||||||
Keywords | TRANSPORT PROTEIN / membrane protein | ||||||
Function / homology | Function and homology information potassium:proton antiporter activity / sodium:proton antiporter activity / xenobiotic detoxification by transmembrane export across the plasma membrane / regulation of cellular pH / response to antibiotic / plasma membrane Similarity search - Function | ||||||
Biological species | Escherichia coli (E. coli) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / SAD / Resolution: 3 Å | ||||||
Authors | Lu, M. / Lu, M.M. | ||||||
Funding support | United States, 1items
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Citation | Journal: Sci Rep / Year: 2020 Title: Structure and mechanism of a redesigned multidrug transporter from the Major Facilitator Superfamily. Authors: Wu, H.H. / Symersky, J. / Lu, M. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 6vs1.cif.gz | 88 KB | Display | PDBx/mmCIF format |
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PDB format | pdb6vs1.ent.gz | 64.4 KB | Display | PDB format |
PDBx/mmJSON format | 6vs1.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/vs/6vs1 ftp://data.pdbj.org/pub/pdb/validation_reports/vs/6vs1 | HTTPS FTP |
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-Related structure data
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
#1: Protein | Mass: 41923.020 Da / Num. of mol.: 1 / Mutation: E26T/D34M/A150E Source method: isolated from a genetically manipulated source Source: (gene. exp.) Escherichia coli (E. coli) / Gene: mdfA, cmlA, cmr, b0842, JW0826 / Production host: Escherichia coli (E. coli) / References: UniProt: P0AEY8 |
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#2: Chemical | ChemComp-PR / |
#3: Chemical | ChemComp-DXC / ( |
Has ligand of interest | Y |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 4.15 Å3/Da / Density % sol: 70.39 % |
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Crystal grow | Temperature: 293 K / Method: vapor diffusion, hanging drop / Details: PEG, salts, etc |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N |
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Diffraction source | Source: SYNCHROTRON / Site: APS / Beamline: 23-ID-B / Wavelength: 1 Å |
Detector | Type: MAR CCD 130 mm / Detector: CCD / Date: Jan 1, 2018 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1 Å / Relative weight: 1 |
Reflection | Resolution: 3→100 Å / Num. obs: 12474 / % possible obs: 97 % / Redundancy: 12 % / CC1/2: 1 / Rsym value: 0.12 / Net I/σ(I): 36 |
Reflection shell | Resolution: 3→3.1 Å / Num. unique obs: 300 / CC1/2: 0.43 / Rsym value: 0.7 / % possible all: 91 |
-Processing
Software |
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Refinement | Method to determine structure: SAD / Resolution: 3→15 Å / Cross valid method: THROUGHOUT
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Displacement parameters | Biso max: 398.86 Å2 / Biso mean: 185.9271 Å2 / Biso min: 69.9 Å2 | ||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 3→15 Å
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