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Yorodumi- PDB-6vjv: Crystal structure of the Prochlorococcus phage (myovirus P-SSM2) ... -
+Open data
-Basic information
Entry | Database: PDB / ID: 6vjv | ||||||||||||
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Title | Crystal structure of the Prochlorococcus phage (myovirus P-SSM2) ferredoxin at 1.6 Angstroms | ||||||||||||
Components | Ferredoxin | ||||||||||||
Keywords | ELECTRON TRANSPORT / Iron Sulfur Cluster Binding / myovirus P-SSM2 / Prochlorococcus phage / ferredoxin / 2 Iron 2 Sulfur | ||||||||||||
Function / homology | Function and homology information 2 iron, 2 sulfur cluster binding / electron transfer activity / metal ion binding Similarity search - Function | ||||||||||||
Biological species | Prochlorococcus phage P-SSM2 (virus) | ||||||||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 1.59 Å | ||||||||||||
Authors | Olmos Jr., J.L. / Campbell, I.J. / Miller, M.D. / Xu, W. / Kahanda, D. / Atkinson, J.T. / Sparks, N. / Bennett, G.N. / Silberg, J.J. / Phillips Jr., G.N. | ||||||||||||
Funding support | United States, 3items
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Citation | Journal: J.Biol.Chem. / Year: 2020 Title: Prochlorococcusphage ferredoxin: structural characterization and electron transfer to cyanobacterial sulfite reductases. Authors: Campbell, I.J. / Olmos Jr., J.L. / Xu, W. / Kahanda, D. / Atkinson, J.T. / Sparks, O.N. / Miller, M.D. / Phillips Jr., G.N. / Bennett, G.N. / Silberg, J.J. | ||||||||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 6vjv.cif.gz | 131.4 KB | Display | PDBx/mmCIF format |
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PDB format | pdb6vjv.ent.gz | 102.3 KB | Display | PDB format |
PDBx/mmJSON format | 6vjv.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/vj/6vjv ftp://data.pdbj.org/pub/pdb/validation_reports/vj/6vjv | HTTPS FTP |
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-Related structure data
Related structure data | 5h57S S: Starting model for refinement |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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2 |
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Unit cell |
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-Components
#1: Protein | Mass: 10381.252 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Prochlorococcus phage P-SSM2 (virus) / Gene: PCMG_00283, PSSM2_281 / Production host: Escherichia coli BL21(DE3) (bacteria) / References: UniProt: Q58M74 #2: Chemical | #3: Chemical | ChemComp-ACT / #4: Chemical | ChemComp-ZN / #5: Water | ChemComp-HOH / | Has ligand of interest | Y | |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.44 Å3/Da / Density % sol: 49.61 % |
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Crystal grow | Temperature: 293 K / Method: vapor diffusion, sitting drop / pH: 4.5 Details: 10% (w/v) PEG 3000, 200 mM zinc acetate, and 100 mM sodium acetate/acetic acid pH 4.5 |
-Data collection
Diffraction | Mean temperature: 100 K / Ambient temp details: cryo-stream / Serial crystal experiment: N | |||||||||||||||||||||||||||
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Diffraction source | Source: SYNCHROTRON / Site: APS / Beamline: 23-ID-B / Wavelength: 1.033 Å | |||||||||||||||||||||||||||
Detector | Type: DECTRIS EIGER X 16M / Detector: PIXEL / Date: Feb 7, 2019 / Details: Adjustable focus K-B pair | |||||||||||||||||||||||||||
Radiation | Monochromator: Si(111) / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray | |||||||||||||||||||||||||||
Radiation wavelength | Wavelength: 1.033 Å / Relative weight: 1 | |||||||||||||||||||||||||||
Reflection | Resolution: 1.589→52.57 Å / Num. obs: 27020 / % possible obs: 98.5 % / Redundancy: 6.6 % / CC1/2: 0.996 / Rmerge(I) obs: 0.12 / Rpim(I) all: 0.05 / Rrim(I) all: 0.131 / Net I/σ(I): 8.9 | |||||||||||||||||||||||||||
Reflection shell | Diffraction-ID: 1
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-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: 5H57 Resolution: 1.59→39.06 Å / SU ML: 0.2 / Cross valid method: THROUGHOUT / σ(F): 1.33 / Phase error: 22.12 Details: In chain B, the iron-sulfur cluster site was disrupted. The density surrounding the cluster in chain B is consistent with a mixture of zinc bound and an intact 2Fe-2S cluster. To account for ...Details: In chain B, the iron-sulfur cluster site was disrupted. The density surrounding the cluster in chain B is consistent with a mixture of zinc bound and an intact 2Fe-2S cluster. To account for the poor density and the anomalous data, chain B has been modeled as a grouped occupancy, where the model is partially comprised of intact cysteine-coordinated iron-sulfur cluster, and a zinc ion with a hydration shell.
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso max: 108.9 Å2 / Biso mean: 33.7873 Å2 / Biso min: 11.18 Å2 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: final / Resolution: 1.59→39.06 Å
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LS refinement shell | Refine-ID: X-RAY DIFFRACTION / Rfactor Rfree error: 0 / Total num. of bins used: 9
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Refinement TLS params. | Method: refined / Refine-ID: X-RAY DIFFRACTION
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Refinement TLS group |
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