the crystallographic asymmetric unit contains two apo IBDs and four IBD-alphaSCS complexes that are arranged as a pseudocontinous helix. Because extra IBD was used in the crystallization, there are extra apo IBDs.
-
Components
#1: DNA chain
ALPHA-SCSINR
Mass: 3597.367 Da / Num. of mol.: 4 / Source method: obtained synthetically / Details: the alpha-SCS Inr promoter element, DNA site
#2: DNA chain
ALPHA-SCSINR
Mass: 3726.453 Da / Num. of mol.: 4 / Source method: obtained synthetically
#3: Protein
39kDainitiatorbindingprotein
Mass: 15344.390 Da / Num. of mol.: 6 / Fragment: residues 1-126 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Trichomonas vaginalis (eukaryote) / Gene: Trichomonas vaginalis:IBP39 / Plasmid: pEQ60 / Production host: Escherichia coli (E. coli) / References: UniProt: Q95VR4
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