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- PDB-6vhj: Solution NMR of Prochlorosin 1.1 produced by Prochlorococcus MIT 9313 -
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Open data
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Basic information
Entry | Database: PDB / ID: 6vhj | ||||||||||||
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Title | Solution NMR of Prochlorosin 1.1 produced by Prochlorococcus MIT 9313 | ||||||||||||
![]() | Prochlorosin 1.1 | ||||||||||||
![]() | UNKNOWN FUNCTION / lanthipeptide / cyclic peptide / posttranslational modification / RiPP | ||||||||||||
Function / homology | Nif11 domain / Nif11-like leader peptide / Nif11 domain / extracellular region / Lantipeptide prochlorosin 1.1![]() | ||||||||||||
Biological species | ![]() | ||||||||||||
Method | SOLUTION NMR / simulated annealing | ||||||||||||
![]() | Bobeica, S.C. / van der Donk, W.A. / Tang, W. | ||||||||||||
Funding support | ![]()
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![]() | ![]() Title: Structural determinants of macrocyclization in substrate-controlled lanthipeptide biosynthetic pathways. Authors: Bobeica, S.C. / Zhu, L. / Acedo, J.Z. / Tang, W. / van der Donk, W.A. #1: Journal: Chem Sci / Year: 2020 Title: Correction: Structural determinants of macrocyclization in substrate-controlled lanthipeptide biosynthetic pathways. Authors: Bobeica, S.C. / Zhu, L. / Acedo, J.Z. / Tang, W. / van der Donk, W.A. #2: Journal: Biochemistry / Year: 2012 Title: Structural characterization of four prochlorosins: a novel class of lantipeptides produced by planktonic marine cyanobacteria. Authors: Tang, W. / van der Donk, W.A. #3: Journal: Nat.Chem.Biol. / Year: 2013 Title: The sequence of the enterococcal cytolysin imparts unusual lanthionine stereochemistry. Authors: Tang, W. / van der Donk, W.A. #4: Journal: Proc.Natl.Acad.Sci.USA / Year: 2010 Title: Catalytic promiscuity in the biosynthesis of cyclic peptide secondary metabolites in planktonic marine cyanobacteria. Authors: Li, B. / Sher, D. / Kelly, L. / Shi, Y. / Huang, K. / Knerr, P.J. / Joewono, I. / Rusch, D. / Chisholm, S.W. / van der Donk, W.A. #5: Journal: Proc.Natl.Acad.Sci.USA / Year: 2017 Title: Evolutionary radiation of lanthipeptides in marine cyanobacteria. Authors: Cubillos-Ruiz, A. / Berta-Thompson, J.W. / Becker, J.W. / van der Donk, W.A. / Chisholm, S.W. | ||||||||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 90.4 KB | Display | ![]() |
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PDB format | ![]() | 62.3 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 444.1 KB | Display | ![]() |
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Full document | ![]() | 607.5 KB | Display | |
Data in XML | ![]() | 22.4 KB | Display | |
Data in CIF | ![]() | 29.7 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 6ve9C ![]() 6vgtC ![]() 6vjqC ![]() 6vljC ![]() 7ju9C ![]() 7jvfC C: citing same article ( |
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Similar structure data | |
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Links
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Assembly
Deposited unit | ![]()
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1 |
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NMR ensembles |
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Components
#1: Protein/peptide | Mass: 1789.109 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() Details (production host): Peptide is purified N-terminally His6-tagged from co-expression with ProcM Production host: ![]() ![]() |
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Has ligand of interest | Y |
-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||||||||||||||||
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NMR experiment |
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Sample preparation
Details |
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Sample |
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Sample conditions | Ionic strength: 0 Not defined / Ionic strength err: _ / pH: 6 / PH err: 0.1 / Pressure: 1 atm / Pressure err: _ / Temperature: 296 K / Temperature err: 0.1
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-NMR measurement
NMR spectrometer | Type: Varian INOVA / Manufacturer: Varian / Model: INOVA / Field strength: 600 MHz |
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Processing
NMR software |
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Refinement | Method: simulated annealing / Software ordinal: 4 | ||||||||||||||||||
NMR representative | Selection criteria: medoid | ||||||||||||||||||
NMR ensemble | Conformer selection criteria: structures with acceptable covalent geometry Conformers calculated total number: 200 / Conformers submitted total number: 20 |