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Open data
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Basic information
Entry | Database: PDB / ID: 6upp | ||||||
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Title | Radiation Damage Test of PixJ Pb state crystals | ||||||
![]() | Methyl-accepting chemotaxis protein | ||||||
![]() | SIGNALING PROTEIN / Phytochrome Chromophore Radiation Damage | ||||||
Function / homology | ![]() chemotaxis / lysozyme activity / signal transduction / metal ion binding / plasma membrane Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Clinger, J.A. / Miller, M.D. / Burgie, E.S. / Vierstra, R.D. / Phillips Jr., G.N. | ||||||
Funding support | ![]()
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![]() | ![]() Title: Photoreversible interconversion of a phytochrome photosensory module in the crystalline state. Authors: Burgie, E.S. / Clinger, J.A. / Miller, M.D. / Brewster, A.S. / Aller, P. / Butryn, A. / Fuller, F.D. / Gul, S. / Young, I.D. / Pham, C.C. / Kim, I.S. / Bhowmick, A. / O'Riordan, L.J. / ...Authors: Burgie, E.S. / Clinger, J.A. / Miller, M.D. / Brewster, A.S. / Aller, P. / Butryn, A. / Fuller, F.D. / Gul, S. / Young, I.D. / Pham, C.C. / Kim, I.S. / Bhowmick, A. / O'Riordan, L.J. / Sutherlin, K.D. / Heinemann, J.V. / Batyuk, A. / Alonso-Mori, R. / Hunter, M.S. / Koglin, J.E. / Yano, J. / Yachandra, V.K. / Sauter, N.K. / Cohen, A.E. / Kern, J. / Orville, A.M. / Phillips Jr., G.N. / Vierstra, R.D. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 204.1 KB | Display | ![]() |
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PDB format | ![]() | 164.5 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 461.5 KB | Display | ![]() |
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Full document | ![]() | 464.3 KB | Display | |
Data in XML | ![]() | 2.4 KB | Display | |
Data in CIF | ![]() | 7.6 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 6p58SC ![]() 6pruC ![]() 6pryC S: Starting model for refinement C: citing same article ( |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 17152.381 Da / Num. of mol.: 2 / Fragment: GAF domain Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() Production host: ![]() ![]() #2: Chemical | #3: Chemical | ChemComp-MG / #4: Chemical | #5: Water | ChemComp-HOH / | Has ligand of interest | Y | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.18 Å3/Da / Density % sol: 43.57 % / Mosaicity: 0 ° |
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Crystal grow | Temperature: 298 K / Method: liquid diffusion / pH: 7.5 / Details: '100mM HEPES pH 7.5, 18% PEG 3350, 200mM MgCl' |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N | ||||||||||||||||||||||||||||||
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Diffraction source | Source: ![]() ![]() ![]() | ||||||||||||||||||||||||||||||
Detector | Type: DECTRIS EIGER X 16M / Detector: PIXEL / Date: Jun 13, 2019 | ||||||||||||||||||||||||||||||
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray | ||||||||||||||||||||||||||||||
Radiation wavelength | Wavelength: 1.033 Å / Relative weight: 1 | ||||||||||||||||||||||||||||||
Reflection | Resolution: 1.56→38.84 Å / Num. obs: 43374 / % possible obs: 99.6 % / Redundancy: 3.3 % / CC1/2: 0.988 / Rmerge(I) obs: 0.115 / Rpim(I) all: 0.075 / Rrim(I) all: 0.138 / Net I/σ(I): 4.9 / Num. measured all: 141510 / Scaling rejects: 832 | ||||||||||||||||||||||||||||||
Reflection shell | Diffraction-ID: 1
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Processing
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Refinement | Method to determine structure: ![]() Starting model: 6P58 Resolution: 1.56→34.575 Å / SU ML: 0.15 / Cross valid method: THROUGHOUT / σ(F): 1.35 / Phase error: 18.71
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso max: 115.66 Å2 / Biso mean: 26.0882 Å2 / Biso min: 8.4 Å2 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: final / Resolution: 1.56→34.575 Å
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LS refinement shell | Refine-ID: X-RAY DIFFRACTION / Rfactor Rfree error: 0
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