- PDB-6uhl: Crystal Structure of C148 mGFP-scDNA-1 -
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基本情報
登録情報
データベース: PDB / ID: 6uhl
タイトル
Crystal Structure of C148 mGFP-scDNA-1
要素
C148 mGFP-scDNA-1
キーワード
FLUORESCENT PROTEIN / green fluorescent protein / beta-barrel / DNA / protein-DNA conjugate
機能・相同性
Green fluorescent protein, GFP / Green fluorescent protein-related / Green fluorescent protein / Green fluorescent protein / bioluminescence / generation of precursor metabolites and energy / Unknown ligand / Green fluorescent protein
分子量: 31038.908 Da / 分子数: 2 / 変異: S148C / 由来タイプ: 組換発現 詳細: This mutant of EGFP has a single surface cysteine at residue 148 (counting from M37 and counting CRO as 3 residues) and an N-terminal his-tag. 由来: (組換発現) Aequorea victoria (オワンクラゲ) 発現宿主: Escherichia coli BL21(DE3) (大腸菌) / 参照: UniProt: P42212*PLUS
解像度: 1.91→43.29 Å / Cor.coef. Fo:Fc: 0.922 / Cor.coef. Fo:Fc free: 0.894 / WRfactor Rfree: 0.2603 / WRfactor Rwork: 0.2151 / FOM work R set: 0.7617 / SU B: 4.698 / SU ML: 0.133 / SU R Cruickshank DPI: 0.1704 / SU Rfree: 0.1623 / 交差検証法: THROUGHOUT / σ(F): 0 / ESU R: 0.17 / ESU R Free: 0.162 / 立体化学のターゲット値: MAXIMUM LIKELIHOOD 詳細: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS U VALUES : REFINED INDIVIDUALLY
Rfactor
反射数
%反射
Selection details
Rfree
0.273
2173
4.9 %
RANDOM
Rwork
0.2282
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obs
0.2304
41766
96.89 %
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溶媒の処理
イオンプローブ半径: 0.8 Å / 減衰半径: 0.8 Å / VDWプローブ半径: 1.2 Å / 溶媒モデル: MASK