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- PDB-6u4y: Crystal Structure of an EZH2-EED Complex in an Oligomeric State -

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Basic information

Entry
Database: PDB / ID: 6u4y
TitleCrystal Structure of an EZH2-EED Complex in an Oligomeric State
Components
  • Histone-lysine N-methyltransferase EZH2
  • Polycomb protein EED
KeywordsTRANSFERASE / TRANSCRIPTION / Methyltransferase
Function / homology
Function and homology information


regulation of kidney development / hepatocyte homeostasis / cellular response to trichostatin A / regulation of gliogenesis / [histone H3]-lysine27 N-trimethyltransferase / negative regulation of striated muscle cell differentiation / negative regulation of keratinocyte differentiation / histone H3K27 trimethyltransferase activity / negative regulation of retinoic acid receptor signaling pathway / primary miRNA binding ...regulation of kidney development / hepatocyte homeostasis / cellular response to trichostatin A / regulation of gliogenesis / [histone H3]-lysine27 N-trimethyltransferase / negative regulation of striated muscle cell differentiation / negative regulation of keratinocyte differentiation / histone H3K27 trimethyltransferase activity / negative regulation of retinoic acid receptor signaling pathway / primary miRNA binding / skeletal muscle satellite cell maintenance involved in skeletal muscle regeneration / response to tetrachloromethane / cerebellar cortex development / histone H3K27 methyltransferase activity / positive regulation of cell cycle G1/S phase transition / facultative heterochromatin formation / chromatin silencing complex / ESC/E(Z) complex / protein-lysine N-methyltransferase activity / negative regulation of stem cell differentiation / pronucleus / cardiac muscle hypertrophy in response to stress / synaptic transmission, GABAergic / G1 to G0 transition / lncRNA binding / positive regulation of dendrite development / histone H3 methyltransferase activity / negative regulation of gene expression, epigenetic / : / negative regulation of G1/S transition of mitotic cell cycle / spinal cord development / histone methyltransferase activity / negative regulation of transcription elongation by RNA polymerase II / Transcriptional Regulation by E2F6 / subtelomeric heterochromatin formation / enzyme activator activity / negative regulation of cytokine production involved in inflammatory response / positive regulation of epithelial to mesenchymal transition / RNA polymerase II core promoter sequence-specific DNA binding / ribonucleoprotein complex binding / pericentric heterochromatin / heterochromatin formation / keratinocyte differentiation / protein localization to chromatin / B cell differentiation / transcription corepressor binding / PRC2 methylates histones and DNA / Regulation of PTEN gene transcription / Defective pyroptosis / liver regeneration / promoter-specific chromatin binding / stem cell differentiation / hippocampus development / G1/S transition of mitotic cell cycle / protein modification process / positive regulation of MAP kinase activity / positive regulation of protein serine/threonine kinase activity / regulation of circadian rhythm / chromatin DNA binding / cellular response to hydrogen peroxide / PKMTs methylate histone lysines / positive regulation of GTPase activity / Activation of anterior HOX genes in hindbrain development during early embryogenesis / HCMV Early Events / transcription corepressor activity / rhythmic process / response to estradiol / chromosome / chromatin organization / Oxidative Stress Induced Senescence / chromosome, telomeric region / RNA polymerase II cis-regulatory region sequence-specific DNA binding / negative regulation of DNA-templated transcription / synapse / chromatin binding / positive regulation of cell population proliferation / chromatin / regulation of DNA-templated transcription / negative regulation of transcription by RNA polymerase II / nucleoplasm / identical protein binding / nucleus / cytosol
Similarity search - Function
EZH2, SET domain / : / Ezh2, MCSS domain / Histone-lysine N-methyltransferase EZH1/EZH2 / Polycomb repressive complex 2 subunit EZH1/EZH2, tri-helical domain / Pre-SET CXC domain / WD repeat binding protein EZH2 / Polycomb repressive complex 2 tri-helical domain / CXC domain / Tesmin/TSO1-like CXC domain ...EZH2, SET domain / : / Ezh2, MCSS domain / Histone-lysine N-methyltransferase EZH1/EZH2 / Polycomb repressive complex 2 subunit EZH1/EZH2, tri-helical domain / Pre-SET CXC domain / WD repeat binding protein EZH2 / Polycomb repressive complex 2 tri-helical domain / CXC domain / Tesmin/TSO1-like CXC domain / Tesmin/TSO1-like CXC domain / Histone-lysine N-methyltransferase EZH1/2-like / CXC domain / CXC domain profile. / SET (Su(var)3-9, Enhancer-of-zeste, Trithorax) domain / SANT SWI3, ADA2, N-CoR and TFIIIB'' DNA-binding domains / SET domain superfamily / SET domain / SANT/Myb domain / SET domain profile. / SET domain / WD40 repeat, conserved site / Trp-Asp (WD) repeats signature. / WD domain, G-beta repeat / WD40 repeats / WD40 repeat / Trp-Asp (WD) repeats profile. / Trp-Asp (WD) repeats circular profile. / WD40-repeat-containing domain superfamily / WD40/YVTN repeat-like-containing domain superfamily
Similarity search - Domain/homology
Polycomb protein EED / Histone-lysine N-methyltransferase EZH2
Similarity search - Component
Biological speciesHomo sapiens (human)
MethodX-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / molecular replacement / Resolution: 2.91 Å
AuthorsJiao, L. / Liu, X.
Funding support United States, 1items
OrganizationGrant numberCountry
National Institutes of Health/National Human Genome Research Institute (NIH/NHGRI) United States
CitationJournal: Proc.Natl.Acad.Sci.USA / Year: 2020
Title: A partially disordered region connects gene repression and activation functions of EZH2.
Authors: Jiao, L. / Shubbar, M. / Yang, X. / Zhang, Q. / Chen, S. / Wu, Q. / Chen, Z. / Rizo, J. / Liu, X.
History
DepositionAug 26, 2019Deposition site: RCSB / Processing site: RCSB
Revision 1.0Jul 8, 2020Provider: repository / Type: Initial release
Revision 1.1Jul 22, 2020Group: Database references / Category: citation / Item: _citation.pdbx_database_id_PubMed / _citation.title
Revision 1.2Aug 12, 2020Group: Database references / Category: citation
Item: _citation.journal_volume / _citation.page_first / _citation.page_last
Revision 1.3Mar 13, 2024Group: Data collection / Database references / Category: chem_comp_atom / chem_comp_bond / database_2
Item: _database_2.pdbx_DOI / _database_2.pdbx_database_accession

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
A: Histone-lysine N-methyltransferase EZH2
B: Histone-lysine N-methyltransferase EZH2
C: Histone-lysine N-methyltransferase EZH2
D: Polycomb protein EED
E: Polycomb protein EED
F: Polycomb protein EED


Theoretical massNumber of molelcules
Total (without water)203,6586
Polymers203,6586
Non-polymers00
Water0
1


  • Idetical with deposited unit
  • defined by author&software
  • Evidence: gel filtration
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area25640 Å2
ΔGint-135 kcal/mol
Surface area69420 Å2
MethodPISA
Unit cell
Length a, b, c (Å)181.632, 114.721, 131.524
Angle α, β, γ (deg.)90.000, 90.000, 90.000
Int Tables number17
Space group name H-MP2221
Space group name HallP2c2

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Components

#1: Protein Histone-lysine N-methyltransferase EZH2 / ENX-1 / Enhancer of zeste homolog 2 / Lysine N-methyltransferase 6


Mass: 25701.156 Da / Num. of mol.: 3 / Fragment: UNP residues 2-182, linker, UNP residues 220-257
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Gene: EZH2, KMT6 / Production host: Escherichia coli (E. coli)
References: UniProt: Q15910, histone-lysine N-methyltransferase
#2: Protein Polycomb protein EED / / hEED / Embryonic ectoderm development protein / WD protein associating with integrin cytoplasmic ...hEED / Embryonic ectoderm development protein / WD protein associating with integrin cytoplasmic tails 1 / WAIT-1


Mass: 42184.965 Da / Num. of mol.: 3 / Fragment: UNP residues 78-441
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Gene: EED / Production host: Escherichia coli (E. coli) / References: UniProt: O75530

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Experimental details

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Experiment

ExperimentMethod: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

CrystalDensity Matthews: 3.36 Å3/Da / Density % sol: 67.38 %
Crystal growTemperature: 298 K / Method: vapor diffusion, hanging drop
Details: 1 M sodium/potassium tartrate, 0.2 M lithium sulfate, 0.1 M Tris-HCl, pH 7.0

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Data collection

DiffractionMean temperature: 93 K / Serial crystal experiment: N
Diffraction sourceSource: SYNCHROTRON / Site: APS / Beamline: 19-ID / Wavelength: 0.97927 Å
DetectorType: ADSC QUANTUM 315r / Detector: CCD / Date: Dec 12, 2015
RadiationMonochromator: double crystal Si(111) / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelengthWavelength: 0.97927 Å / Relative weight: 1
ReflectionResolution: 2.9→50 Å / Num. obs: 54528 / % possible obs: 99.8 % / Redundancy: 13.7 % / Biso Wilson estimate: 53.78 Å2 / CC1/2: 0.994 / Rmerge(I) obs: 0.12 / Rpim(I) all: 0.052 / Net I/σ(I): 15.1
Reflection shellResolution: 2.9→2.95 Å / Rmerge(I) obs: 1.329 / Mean I/σ(I) obs: 1 / Num. unique obs: 2382 / CC1/2: 0.561 / Rpim(I) all: 0.579

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Phasing

PhasingMethod: molecular replacement

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Processing

Software
NameVersionClassification
BUSTER2.10.3refinement
PHENIX1.16_3549refinement
HKL-2000data scaling
PHASERphasing
PDB_EXTRACT3.25data extraction
HKL-2000data reduction
RefinementMethod to determine structure: MOLECULAR REPLACEMENT / Resolution: 2.91→48.5 Å / SU ML: 0.2878 / Cross valid method: FREE R-VALUE / σ(F): 1.34 / Phase error: 23.1215
Stereochemistry target values: GeoStd + Monomer Library + CDL v1.2
RfactorNum. reflection% reflectionSelection details
Rfree0.2243 2576 4.73 %RANDOM
Rwork0.182 51939 --
obs0.184 54515 89.08 %-
Solvent computationShrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL
Displacement parametersBiso mean: 57.03 Å2
Refinement stepCycle: LAST / Resolution: 2.91→48.5 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms12736 0 0 0 12736
Refine LS restraints
Refine-IDTypeDev idealNumber
X-RAY DIFFRACTIONf_bond_d0.004213021
X-RAY DIFFRACTIONf_angle_d0.700317573
X-RAY DIFFRACTIONf_chiral_restr0.04731895
X-RAY DIFFRACTIONf_plane_restr0.00342236
X-RAY DIFFRACTIONf_dihedral_angle_d7.50127814
LS refinement shell
Resolution (Å)Rfactor RfreeNum. reflection RfreeRfactor RworkNum. reflection RworkRefine-ID% reflection obs (%)
2.91-2.960.3423490.29441140X-RAY DIFFRACTION35.4
2.96-3.020.4157730.27041429X-RAY DIFFRACTION44.73
3.02-3.090.2871780.24671801X-RAY DIFFRACTION56.31
3.09-3.160.31351180.23392436X-RAY DIFFRACTION76.42
3.16-3.240.2641460.24032931X-RAY DIFFRACTION91.41
3.24-3.330.26431480.22853129X-RAY DIFFRACTION97.82
3.33-3.430.29651490.21673228X-RAY DIFFRACTION99.53
3.43-3.540.22391460.19073216X-RAY DIFFRACTION99.97
3.54-3.660.25441420.1783211X-RAY DIFFRACTION100
3.66-3.810.2251600.1743244X-RAY DIFFRACTION100
3.81-3.980.22561560.16423224X-RAY DIFFRACTION99.97
3.98-4.190.2231680.1553214X-RAY DIFFRACTION99.97
4.19-4.450.17081510.14273237X-RAY DIFFRACTION99.97
4.45-4.80.18111880.13563229X-RAY DIFFRACTION100
4.8-5.280.16141680.13933252X-RAY DIFFRACTION100
5.28-6.040.23641700.18383289X-RAY DIFFRACTION100
6.04-7.610.21421740.19633307X-RAY DIFFRACTION100
7.61-48.50.22841920.21123422X-RAY DIFFRACTION99.37
Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

IDL11 (°2)L12 (°2)L13 (°2)L22 (°2)L23 (°2)L33 (°2)S11 (Å °)S12 (Å °)S13 (Å °)S21 (Å °)S22 (Å °)S23 (Å °)S31 (Å °)S32 (Å °)S33 (Å °)T11 (Å2)T12 (Å2)T13 (Å2)T22 (Å2)T23 (Å2)T33 (Å2)Origin x (Å)Origin y (Å)Origin z (Å)
11.03804194734-1.866783799140.1700164912165.69203905699-0.395105145570.8042787921740.167250249366-0.3036293486820.1427225368650.292020245323-0.192525382302-0.3618782279910.09598869264420.201694186585-0.1249485375110.34733865375-0.068158251441-0.0593473587780.728988085482-0.1067850860380.49045383033393.92226325153.26855219782286.57473824
22.919013026620.197597800206-0.6219246013822.00782585994-0.3297279442841.646027479920.247604536959-0.1108415392660.8192450587050.0659776216254-0.05909285474340.242103003682-0.2392079381410.0160841995378-0.06133456994520.5569204688750.04107374362370.1044472382030.388623154854-0.1423276782770.48245661809858.76786636135.4141481942280.874467509
31.18709316908-0.547060720149-0.645486024071.505717203770.5250846326030.361755193164-0.1284521085290.1868624591770.588801424775-0.2742599289890.43411009696-0.0815862540259-0.8232580735480.339623229349-0.03323577983631.21807034907-0.06821515894720.2297754448250.793669510608-0.004335077895781.042582052549.379808388947.6394418976242.09519514
46.07196451191-0.1600026861461.932876275130.2743968137230.3016230468751.42027944570.31574162292-0.587414106373-0.5031296912260.351523401806-0.3178577786860.2448546662080.152630007273-0.1637925893880.06650198124080.431307754082-0.1021403509950.04000052043030.5659036776540.3208981359240.48386902721419.6881105194-21.9826674826298.784007676
51.792451534720.02384113858320.1094572583572.5168015605-0.7029962760181.07286267546-0.05471798753-0.250546218284-0.08204685330290.2023191020520.082780276429-0.6006714940470.3303473493660.0574849059267-0.007830690586320.327745671686-0.0255072624046-0.08607065999620.5046102400360.01711848510290.347482448165.4972908732-8.95390722208297.679299162
63.173244309161.409355749421.385460279171.736111338681.331209291971.989272261830.0762107472222-0.3852619598-0.3078452799750.116004754269-0.0407439405691-0.03330061251180.311712019831-0.140310801576-0.1977638328680.544417044715-0.0286935489362-0.1703625221290.569418204483-0.003483176362780.47715665613295.66480916-23.0658437193277.221312307
71.433926386560.3989408774290.01234662434490.8594047827460.1455520561921.041279164070.0347847506196-0.2331270162060.344511625164-0.135502616725-0.02300954089350.261977785485-0.214793251812-0.2186404057540.04543741249880.4835374179970.166324687524-0.03678033177270.367836946441-0.1047545193340.42376693183524.442631046726.9943233512274.318031395
83.315775040080.477043839652-0.7145406873241.88928947195-0.3594710330932.77482001623-0.3145735033290.464417245257-0.586429324018-0.2876135915030.3202613579910.00376453038720.110246908743-0.241336381760.2720378935920.461610977735-0.05491554645520.1608511913270.672472073948-0.08204471724670.5934238096982.94244381972-26.5222238386281.276030251
93.331418772030.313542483492-0.7200975536553.2986146930.01805131923283.062104248030.332038816622-0.5082145239880.7218046290580.1757700156130.2801002247780.181564257841-0.813359496277-0.07007681252810.02158649844640.337690038373-0.05373492037820.09553263254260.239887285738-0.1956805880140.30210650401864.850598996327.9304134902283.116406739
102.503375951981.06347586022-0.6019541333144.192741898731.637241255543.303195908050.0118407162490.3864901912221.15131459684-0.4832315508470.1576602057120.243647067802-1.280431326080.0817027267023-0.1483403556520.607572215775-0.02117996372730.1015164303810.220062549411-0.003364985720970.59984722249868.742162886936.9657466587270.528960732
115.779490110460.637563151538-0.1597848943094.32464362809-0.03977068955134.055793995560.2529293698930.4802705525680.674907412658-0.402903825181-0.0648597631027-0.151387822262-0.512213490290.215063714985-0.2698539778550.356000007422-0.07769854776530.1150516341330.261814876494-0.003178865721770.28386545718876.221303554126.9925882255267.802359937
124.795334436170.525809168373-0.4513433183132.99461114208-0.5061990910212.561423984660.0789770592570.7731915866971.05252666038-0.809228852298-0.1300559841-0.00205241753661-0.433646879850.0909237078156-0.3079504318210.518878407426-0.08039729050990.1370195168490.3759605049220.02015449597310.34321404226277.750634443828.1124995935261.222124732
134.05457047688-0.175065568684-0.943088316841.60811327418-0.8011705892443.50493547723-0.1643849837960.41735882216-0.0385985244728-0.5078345631830.273862254932-0.5283945941070.1041800609420.423225112678-0.1081605545220.305446241681-0.08610776968160.1290171122490.36776332396-0.08718567270750.32892186550982.581367517516.6771578867266.403702697
143.82553099792-0.007151059990330.07611397781523.083926290390.2500063248293.29157204522-0.04202585329310.6851681088140.604583297964-0.812246701996-0.0649560010641-0.828324165763-0.7847949099510.22724262519-0.3277845248960.596652018091-0.154130931760.2270737206760.655572723743-0.001681839145310.50655921868586.938325825429.1789971523258.570578433
154.395871334410.62485203657-1.472247620692.62483948462-0.1297358194973.73945229267-0.001336192154420.172572298913-0.54638645082-0.1202986369410.0642024231439-0.6133991116580.2522879142740.6669594510130.02724914841410.3162955762940.02860571499460.02263669777060.360674893227-0.1557846590640.38323780148785.56139946868.2715157146274.313496766
163.678211160390.164922051037-1.740190938122.70706854203-0.8462812530722.855326339280.128186616087-0.2360008869690.18645431676-0.118477378813-0.154095159542-0.141158700246-0.1650936854240.504294062118-0.05462945187370.241614730150.008799155552510.03921847891370.276253805108-0.07883723472060.19787617404873.978646190814.2372837229282.403610358
176.267469285791.58769502187-0.69734874452.420575371750.6285428177253.033416735350.181503878465-1.50346861264-0.6536967997580.523207022713-0.176464302881-0.5154023315050.4126054715630.409477395546-0.00887036381950.3369047580060.0424346150057-0.1015050230630.498469718382-0.0004965751900720.14847819902576.37484868879.31881538969290.284715847
183.265559936530.653958628082-0.2271851991252.80683609075-0.1572958675753.060929729080.249470441027-0.4367985102650.2360432287470.507387285190.0213505373888-0.211417928688-0.2008309880570.350559051337-0.1173894064080.263548433505-0.02039604201890.01452460915880.265433341091-0.1368356138640.24490679176966.815182135818.6689725302288.309263892
194.706276721650.624517496225-0.4115450282894.311621897420.838424067124.160354819020.332710985156-0.926995811422-0.05889190222630.440332350132-0.118492599792-0.405125129516-0.130706525368-0.117355132439-0.02959909815790.274705593375-0.08046371194290.06976318788630.414876107579-0.2305047721260.13260889465165.532668888320.798429465290.554175592
203.16493018312-0.2756179157590.1938826555433.34014001789-0.1337966448442.265241702580.0885006692019-0.517041091944-0.08570024896740.3173354366490.0243174116961-0.323748849776-0.01098818750280.136194638742-0.02250332652740.229701024114-0.0630732438448-0.1482952376680.4522319718710.1145900952130.24646367409156.4846334311-8.16216194462300.089723509
212.821855578110.9677767486942.41973064272.672690725912.243470480136.718225611220.4872970428480.325177757061-0.4889820891260.266862162153-0.00812737670389-0.7801389644320.2622048339160.91546765641-0.1685425709910.238830703054-0.0160156394686-0.1098043878490.5146150331110.03128441708920.5284499615766.4037781096-17.9871800384294.128358552
222.74783953570.007694575161530.3183638564273.15745720739-0.430388462293.034295790670.352104114064-0.0326784144278-0.7003682349760.007109326010550.1041610898420.006002796827860.3895462241450.379966014381-0.2085764161580.3696409088860.0237856389643-0.1610939088910.3860856839440.1011916629180.39985480706754.5584026942-24.1862781342292.670523374
236.51665066888-0.6681724667310.9817400226852.80331709422-2.097140525354.18981129695-0.0836744613092-0.539291471046-1.10597624775-0.347606189567-0.235801565452-0.3431802197490.1937466672160.640811606951-0.7664185354150.4761212054740.0755444412266-0.1872981803830.3514441813470.02055033913160.5162282704457.0304182915-29.5381919711289.099007338
242.7161280723-0.186291707717-0.2171109219833.455059705680.02443847777242.957447528470.237262944793-0.13272937131-0.900343364419-0.282469648881-0.07496352338520.2455175726190.949928336110.35925917438-0.2678334457150.5675321919960.0176794920354-0.1868938863670.3475469270610.1727688763550.59116241759248.5578461225-32.5721058137289.872420053
253.730897319990.387747976808-0.4099594116964.45667424982-0.4732052800715.494368660410.122726925801-0.0223181671314-0.485341363864-0.5056586934630.04159662935980.5520231722770.665296687283-0.359799619654-0.2166649105630.348027023716-0.0710808288924-0.1483758650530.3862084244040.1334164891250.41019435650336.6740328843-20.5384526643289.167258473
261.887041770550.9239625145820.4099246385074.224983022240.4133574040021.911440794580.0233093553691-0.43944169296-0.1288780486010.00474289428380.04299600181410.6296954347290.120309945124-0.433376507591-0.1129406644160.255257912924-0.0371740101369-0.09662687868440.5119754400240.164591828670.32187484712737.7264285638-11.7444963149296.500293518
273.217028233270.2183849058320.8141486433241.80463163682-0.2653164625633.192212121350.0278700934878-0.273612277368-0.06004837627750.0633955889514-0.04403709382650.3541127418140.0825404066456-0.7680924510730.001543758657240.22261487290.07004544083760.01389757201720.340823146307-0.03394514038470.32687518718920.71073477318.0780192689278.222444952
283.30453768686-0.549588766096-1.071065609612.62351990637-0.01370818560834.12857768551-0.3896911959650.918935039188-0.2247606098960.025959529490.0776384644841.024079759940.463695483463-1.52989737213-0.07557355307110.4880718314320.140684987167-0.1478769395351.03790032443-0.06780125585480.6119711404086.3068006752121.43150325262.566973373
294.33690890993-0.4376282760991.150712691572.59361418497-0.1922057413634.207995723-0.07863614630060.4859024750050.634895813562-0.334558498502-0.1474998492510.0791768842768-0.624948497524-0.3955778461570.2222264230110.512344809510.125971269244-0.05674457516590.3265906661260.009233306266960.38063718314328.473179805634.0021383184262.756306271
303.274688853110.2793032491321.20050377441.82547216284-0.9384398937143.07998585477-0.10264161171-0.3125895945840.5438759919960.139063870689-0.02928443608330.100781554615-0.563056496496-0.1130071032180.1650972168950.3418684909410.0411584795328-0.003744053145560.234754262159-0.08665538031590.26417056841535.29934066628.6502823612281.408674134
Refinement TLS group
IDRefine-IDRefine TLS-IDSelection detailsAuth asym-IDAuth seq-ID
1X-RAY DIFFRACTION1CHAIN 'A' AND (RESID 12 THROUGH 62 )A0
2X-RAY DIFFRACTION2CHAIN 'A' AND (RESID 63 THROUGH 107 )A0
3X-RAY DIFFRACTION3CHAIN 'A' AND (RESID 108 THROUGH 249 )A0
4X-RAY DIFFRACTION4CHAIN 'B' AND (RESID 12 THROUGH 60 )B0
5X-RAY DIFFRACTION5CHAIN 'B' AND (RESID 61 THROUGH 112 )B0
6X-RAY DIFFRACTION6CHAIN 'B' AND (RESID 113 THROUGH 249 )B0
7X-RAY DIFFRACTION7CHAIN 'C' AND (RESID 16 THROUGH 144 )C0
8X-RAY DIFFRACTION8CHAIN 'C' AND (RESID 145 THROUGH 249 )C0
9X-RAY DIFFRACTION9CHAIN 'D' AND (RESID 76 THROUGH 154 )D0
10X-RAY DIFFRACTION10CHAIN 'D' AND (RESID 155 THROUGH 186 )D0
11X-RAY DIFFRACTION11CHAIN 'D' AND (RESID 187 THROUGH 210 )D0
12X-RAY DIFFRACTION12CHAIN 'D' AND (RESID 211 THROUGH 229 )D0
13X-RAY DIFFRACTION13CHAIN 'D' AND (RESID 230 THROUGH 267 )D0
14X-RAY DIFFRACTION14CHAIN 'D' AND (RESID 268 THROUGH 301 )D0
15X-RAY DIFFRACTION15CHAIN 'D' AND (RESID 302 THROUGH 357 )D0
16X-RAY DIFFRACTION16CHAIN 'D' AND (RESID 358 THROUGH 380 )D0
17X-RAY DIFFRACTION17CHAIN 'D' AND (RESID 381 THROUGH 404 )D0
18X-RAY DIFFRACTION18CHAIN 'D' AND (RESID 405 THROUGH 423 )D0
19X-RAY DIFFRACTION19CHAIN 'D' AND (RESID 424 THROUGH 441 )D0
20X-RAY DIFFRACTION20CHAIN 'E' AND (RESID 76 THROUGH 167 )E0
21X-RAY DIFFRACTION21CHAIN 'E' AND (RESID 168 THROUGH 186 )E0
22X-RAY DIFFRACTION22CHAIN 'E' AND (RESID 187 THROUGH 210 )E0
23X-RAY DIFFRACTION23CHAIN 'E' AND (RESID 211 THROUGH 229 )E0
24X-RAY DIFFRACTION24CHAIN 'E' AND (RESID 230 THROUGH 301 )E0
25X-RAY DIFFRACTION25CHAIN 'E' AND (RESID 302 THROUGH 322 )E0
26X-RAY DIFFRACTION26CHAIN 'E' AND (RESID 323 THROUGH 440 )E0
27X-RAY DIFFRACTION27CHAIN 'F' AND (RESID 77 THROUGH 267 )F0
28X-RAY DIFFRACTION28CHAIN 'F' AND (RESID 268 THROUGH 301 )F0
29X-RAY DIFFRACTION29CHAIN 'F' AND (RESID 302 THROUGH 357 )F0
30X-RAY DIFFRACTION30CHAIN 'F' AND (RESID 358 THROUGH 440 )F0

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