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Yorodumi- PDB-6tws: Crystal structure of the haemagglutinin mutant (Gln226Leu, Gly228... -
+Open data
-Basic information
Entry | Database: PDB / ID: 6tws | ||||||
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Title | Crystal structure of the haemagglutinin mutant (Gln226Leu, Gly228Ser) from an H10N7 seal influenza virus isolated in Germany with 2mM EDTA | ||||||
Components |
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Keywords | VIRAL PROTEIN / receptor binding / fusion of virus membrane with host plasma membrane | ||||||
Function / homology | Function and homology information clathrin-dependent endocytosis of virus by host cell / host cell surface receptor binding / apical plasma membrane / fusion of virus membrane with host plasma membrane / fusion of virus membrane with host endosome membrane / viral envelope / virion attachment to host cell / host cell plasma membrane / metal ion binding Similarity search - Function | ||||||
Biological species | Influenza A virus | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2 Å | ||||||
Authors | Zhang, J. / Xiong, X. / Purkiss, A. / Walker, P. / Gamblin, S. / Skehel, J.J. | ||||||
Funding support | United Kingdom, 1items
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Citation | Journal: Cell Host Microbe / Year: 2020 Title: Hemagglutinin Traits Determine Transmission of Avian A/H10N7 Influenza Virus between Mammals. Authors: Herfst, S. / Zhang, J. / Richard, M. / McBride, R. / Lexmond, P. / Bestebroer, T.M. / Spronken, M.I.J. / de Meulder, D. / van den Brand, J.M. / Rosu, M.E. / Martin, S.R. / Gamblin, S.J. / ...Authors: Herfst, S. / Zhang, J. / Richard, M. / McBride, R. / Lexmond, P. / Bestebroer, T.M. / Spronken, M.I.J. / de Meulder, D. / van den Brand, J.M. / Rosu, M.E. / Martin, S.R. / Gamblin, S.J. / Xiong, X. / Peng, W. / Bodewes, R. / van der Vries, E. / Osterhaus, A.D.M.E. / Paulson, J.C. / Skehel, J.J. / Fouchier, R.A.M. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 6tws.cif.gz | 324.9 KB | Display | PDBx/mmCIF format |
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PDB format | pdb6tws.ent.gz | 261.3 KB | Display | PDB format |
PDBx/mmJSON format | 6tws.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 6tws_validation.pdf.gz | 1.3 MB | Display | wwPDB validaton report |
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Full document | 6tws_full_validation.pdf.gz | 1.3 MB | Display | |
Data in XML | 6tws_validation.xml.gz | 63.7 KB | Display | |
Data in CIF | 6tws_validation.cif.gz | 92.1 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/tw/6tws ftp://data.pdbj.org/pub/pdb/validation_reports/tw/6tws | HTTPS FTP |
-Related structure data
Related structure data | 6tjwC 6tjyC 6tvaC 6tvbC 6tvcC 6tvdC 6tvfC 6tvrC 6tvsC 6tvtC 6twhC 6twiC 6twvC 6txoC 6ty1C 4d00S S: Starting model for refinement C: citing same article (ref.) |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
-Protein , 2 types, 6 molecules GACHBD
#1: Protein | Mass: 35514.137 Da / Num. of mol.: 3 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Influenza A virus (A/harbour seal/Germany/1/2014(H10N7)) Gene: HA / Cell line (production host): Sf9 / Production host: Spodoptera frugiperda (fall armyworm) / References: UniProt: A0A0A7HR51 #2: Protein | Mass: 20313.389 Da / Num. of mol.: 3 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Influenza A virus (A/harbour seal/Germany/1/2014(H10N7)) Gene: HA / Cell line (production host): Sf9 / Production host: Spodoptera frugiperda (fall armyworm) / References: UniProt: A0A0A7HR51 |
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-Sugars , 2 types, 5 molecules
#3: Polysaccharide | #5: Sugar | |
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-Non-polymers , 2 types, 1123 molecules
#4: Chemical | ChemComp-EDO / #6: Water | ChemComp-HOH / | |
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-Details
Has ligand of interest | N |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 3.1 Å3/Da / Density % sol: 59.6 % |
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Crystal grow | Temperature: 298 K / Method: vapor diffusion, sitting drop / Details: 6% PEG3350, 0.1 M MES pH6.5 |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N |
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Diffraction source | Source: SYNCHROTRON / Site: Diamond / Beamline: I03 / Wavelength: 0.9763 Å |
Detector | Type: DECTRIS PILATUS3 6M / Detector: PIXEL / Date: Feb 4, 2017 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.9763 Å / Relative weight: 1 |
Reflection | Resolution: 2→91.41 Å / Num. obs: 131081 / % possible obs: 98.8 % / Redundancy: 7 % / CC1/2: 0.998 / Net I/σ(I): 12.7 |
Reflection shell | Resolution: 2→2.03 Å / Mean I/σ(I) obs: 3.3 / Num. unique obs: 6478 / CC1/2: 0.972 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: 4D00 Resolution: 2→61.41 Å / Cor.coef. Fo:Fc: 0.95 / Cor.coef. Fo:Fc free: 0.938 / Occupancy max: 1 / Occupancy min: 0.42 / Cross valid method: THROUGHOUT / σ(F): 0 / ESU R: 0.179 / ESU R Free: 0.151 / Stereochemistry target values: MAXIMUM LIKELIHOOD Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS U VALUES : REFINED INDIVIDUALLY
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å / Solvent model: MASK | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso max: 114.57 Å2 / Biso mean: 40.9037 Å2 / Biso min: 20.18 Å2
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Refinement step | Cycle: LAST / Resolution: 2→61.41 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 2→2.052 Å / Total num. of bins used: 20
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