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- PDB-6tpc: Crystal structure of Endoglucanase N194A from Penicillium verruculosum -
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Open data
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Basic information
Entry | Database: PDB / ID: 6tpc | ||||||
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Title | Crystal structure of Endoglucanase N194A from Penicillium verruculosum | ||||||
![]() | Endoglucanase | ||||||
![]() | HYDROLASE / endohydrolysis / cellulose | ||||||
Function / homology | Glycoside hydrolase, family 5 / Cellulase (glycosyl hydrolase family 5) / cellulase / cellulose catabolic process / hydrolase activity, hydrolyzing O-glycosyl compounds / Glycoside hydrolase superfamily / PHOSPHATE ION / cellulase![]() | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Nemashkalov, V. / Kravchenko, O. / Gabdulkhakov, A. / Tischenko, S. / Rozhkova, A. / Sinitsyn, A. | ||||||
Funding support | ![]()
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![]() | ![]() Title: Crystal structure of Endoglucanase N194A from Penicillium verruculosum Authors: Nemashkalov, V. / Kravchenko, O. / Gabdulkhakov, A. / Tischenko, S. / Rozhkova, A. / Sinitsyn, A. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 277.8 KB | Display | ![]() |
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PDB format | ![]() | 213.5 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 1 MB | Display | ![]() |
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Full document | ![]() | 1 MB | Display | |
Data in XML | ![]() | 26.4 KB | Display | |
Data in CIF | ![]() | 39.1 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 5i6sS S: Starting model for refinement |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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2 | ![]()
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Unit cell |
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Components on special symmetry positions |
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Components
#1: Protein | Mass: 34602.781 Da / Num. of mol.: 2 / Mutation: N194A Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() #2: Polysaccharide | Source method: isolated from a genetically manipulated source #3: Chemical | ChemComp-SO4 / | #4: Chemical | ChemComp-PO4 / | #5: Water | ChemComp-HOH / | Has ligand of interest | Y | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.18 Å3/Da / Density % sol: 43.57 % |
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Crystal grow | Temperature: 285 K / Method: vapor diffusion, hanging drop Details: PEG 6000, Bis-Tris-Propan,20% PEG 6000, MPD, tert-butano |
-Data collection
Diffraction | Mean temperature: 110 K / Serial crystal experiment: N |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: DECTRIS EIGER X 16M / Detector: PIXEL / Date: Oct 15, 2019 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.978565 Å / Relative weight: 1 |
Reflection | Resolution: 1.522→48.652 Å / Num. obs: 90739 / % possible obs: 97.4 % / Redundancy: 7.56 % / Biso Wilson estimate: 16.1128787453 Å2 / CC1/2: 0.996 / Net I/σ(I): 8.24 |
Reflection shell | Resolution: 1.522→1.61 Å / Num. unique obs: 14123 / CC1/2: 0.861 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: 5I6S Resolution: 1.52218948487→48.6515593423 Å / SU ML: 0.193301673141 / Cross valid method: FREE R-VALUE / σ(F): 1.32584533157 / Phase error: 28.8661653177
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 19.6305504444 Å2 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 1.52218948487→48.6515593423 Å
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Refine LS restraints |
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LS refinement shell |
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