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- PDB-6tlx: Crystal structure of the unconventional kinetochore protein Perki... -
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Open data
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Basic information
Entry | Database: PDB / ID: 6tlx | ||||||
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Title | Crystal structure of the unconventional kinetochore protein Perkinsela sp. KKT2a central domain | ||||||
![]() | Protein kinase![]() | ||||||
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Function / homology | ![]() | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Marciano, G. / Nerusheva, O. / Ishii, M. / Akiyoshi, B. | ||||||
Funding support | ![]()
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![]() | ![]() Title: Kinetoplastid kinetochore proteins KKT2 and KKT3 have unique centromere localization domains. Authors: Marciano, G. / Ishii, M. / Nerusheva, O.O. / Akiyoshi, B. #1: ![]() Title: Unconventional kinetochore kinases KKT2 and KKT3 have a unique zinc finger that promotes their kinetochore localization Authors: Marciano, G. / Nerusheva, O. / Ishii, M. / Akiyoshi, B. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 36.7 KB | Display | ![]() |
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PDB format | ![]() | 22.7 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: Protein | ![]() Mass: 14570.530 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() | ||
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#2: Chemical | Has ligand of interest | Y | |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 5.91 Å3/Da / Density % sol: 79.18 % |
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Crystal grow![]() | Temperature: 277 K / Method: vapor diffusion, sitting drop / pH: 7.5 / Details: 19% MPD, 50 mM Hepes, pH 7.5, 10 mM MgCl2 |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N | ||||||||||||||||||||||||
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Diffraction source | Source: ![]() ![]() ![]() | ||||||||||||||||||||||||
Detector | Type: DECTRIS PILATUS3 6M / Detector: PIXEL / Date: Feb 12, 2018 | ||||||||||||||||||||||||
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray | ||||||||||||||||||||||||
Radiation wavelength | Wavelength![]() | ||||||||||||||||||||||||
Reflection | Resolution: 2.87→56.92 Å / Num. obs: 7986 / % possible obs: 99.7 % / Redundancy: 19.2 % / Biso Wilson estimate: 105.112 Å2 / CC1/2: 1 / Rmerge(I) obs: 0.102 / Rpim(I) all: 0.024 / Rrim(I) all: 0.105 / Net I/σ(I): 16.4 | ||||||||||||||||||||||||
Reflection shell | Diffraction-ID: 1
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-Phasing
Phasing![]() | Method: ![]() |
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Processing
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Refinement | Method to determine structure![]() ![]()
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Displacement parameters | Biso max: 163.08 Å2 / Biso mean: 113.37 Å2 / Biso min: 87.09 Å2
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Refine analyze | Luzzati coordinate error obs: 0.56 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: final / Resolution: 2.87→49 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 2.87→3 Å / Rfactor Rfree error: 0 / Total num. of bins used: 18
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