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Yorodumi- PDB-6sw5: Crystal structure of the human S-adenosylmethionine synthetase 1 ... -
+Open data
-Basic information
Entry | Database: PDB / ID: 6sw5 | ||||||
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Title | Crystal structure of the human S-adenosylmethionine synthetase 1 (ligand-free form) | ||||||
Components | S-adenosylmethionine synthase isoform type-1 | ||||||
Keywords | TRANSFERASE / S-adenosylmethionine synthesis | ||||||
Function / homology | Function and homology information Defective MAT1A causes MATD / methionine catabolic process / Sulfur amino acid metabolism / methionine adenosyltransferase complex / Metabolism of ingested SeMet, Sec, MeSec into H2Se / methionine adenosyltransferase / methionine adenosyltransferase activity / S-adenosylmethionine biosynthetic process / Methylation / one-carbon metabolic process ...Defective MAT1A causes MATD / methionine catabolic process / Sulfur amino acid metabolism / methionine adenosyltransferase complex / Metabolism of ingested SeMet, Sec, MeSec into H2Se / methionine adenosyltransferase / methionine adenosyltransferase activity / S-adenosylmethionine biosynthetic process / Methylation / one-carbon metabolic process / protein homotetramerization / ATP binding / identical protein binding / metal ion binding / cytosol Similarity search - Function | ||||||
Biological species | Homo sapiens (human) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2.35 Å | ||||||
Authors | Panmanee, J. / Antoyuk, S.V. / Hasnain, S.S. | ||||||
Citation | Journal: Acta Crystallogr D Struct Biol / Year: 2020 Title: Structural basis of the dominant inheritance of hypermethioninemia associated with the Arg264His mutation in the MAT1A gene. Authors: Panmanee, J. / Antonyuk, S.V. / Hasnain, S.S. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 6sw5.cif.gz | 292.2 KB | Display | PDBx/mmCIF format |
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PDB format | pdb6sw5.ent.gz | 236.7 KB | Display | PDB format |
PDBx/mmJSON format | 6sw5.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/sw/6sw5 ftp://data.pdbj.org/pub/pdb/validation_reports/sw/6sw5 | HTTPS FTP |
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-Related structure data
Related structure data | 6sw6C 2obvS S: Starting model for refinement C: citing same article (ref.) |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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2 |
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Unit cell |
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-Components
#1: Protein | Mass: 43703.852 Da / Num. of mol.: 4 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Tissue: Liver / Cell: Hepatocyte / Gene: MAT1A, AMS1, MATA1 / Organ: Liver / Plasmid: pNIC28-Bsa4 / Production host: Escherichia coli BL21(DE3) (bacteria) / References: UniProt: Q00266, methionine adenosyltransferase #2: Chemical | #3: Chemical | ChemComp-EDO / #4: Water | ChemComp-HOH / | Has ligand of interest | N | |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.49 Å3/Da / Density % sol: 50.56 % / Description: Plate shape |
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Crystal grow | Temperature: 298 K / Method: vapor diffusion, hanging drop / pH: 7.5 / Details: 200 mM NaF, 20 % PEG 3350 and 15 % ethylene glycol |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N |
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Diffraction source | Source: SYNCHROTRON / Site: Diamond / Beamline: I04 / Wavelength: 0.9795 Å |
Detector | Type: DECTRIS EIGER2 X 16M / Detector: PIXEL / Date: Jun 29, 2019 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.9795 Å / Relative weight: 1 |
Reflection | Resolution: 2.35→58.76 Å / Num. obs: 66266 / % possible obs: 100 % / Redundancy: 3.4 % / Biso Wilson estimate: 29.335 Å2 / CC1/2: 0.991 / Rmerge(I) obs: 0.127 / Rpim(I) all: 0.08 / Rrim(I) all: 0.15 / Net I/σ(I): 6.4 |
Reflection shell | Resolution: 2.35→2.41 Å / Redundancy: 3.4 % / Rmerge(I) obs: 0.58 / Mean I/σ(I) obs: 1.9 / Num. unique obs: 4425 / CC1/2: 0.762 / Rpim(I) all: 0.365 / Rrim(I) all: 0.686 / % possible all: 99.9 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: 2OBV Resolution: 2.35→58.76 Å / Cor.coef. Fo:Fc: 0.949 / Cor.coef. Fo:Fc free: 0.92 / SU B: 8.642 / SU ML: 0.195 / Cross valid method: THROUGHOUT / σ(F): 0 / ESU R: 0.383 / ESU R Free: 0.246 Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS U VALUES : REFINED INDIVIDUALLY
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso max: 127.88 Å2 / Biso mean: 35.909 Å2 / Biso min: 1 Å2
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Refinement step | Cycle: final / Resolution: 2.35→58.76 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 2.35→2.411 Å / Rfactor Rfree error: 0 / Total num. of bins used: 20
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