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Yorodumi- PDB-6sta: Crystal structure of the strawberry pathogenesis-related 10 (PR-1... -
+Open data
-Basic information
Entry | Database: PDB / ID: 6sta | |||||||||
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Title | Crystal structure of the strawberry pathogenesis-related 10 (PR-10) Fra a 1.02 protein, E46A D48A mutant | |||||||||
Components | Major strawberry allergen Fra a 1-2 | |||||||||
Keywords | ALLERGEN / Fragaria x ananassa / Fra a 1 / fruit / ripening / PR-10 | |||||||||
Function / homology | Function and homology information flavonoid biosynthetic process / response to biotic stimulus / abscisic acid binding / abscisic acid-activated signaling pathway / protein phosphatase inhibitor activity / defense response / signaling receptor activity Similarity search - Function | |||||||||
Biological species | Fragaria ananassa (strawberry) | |||||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2.19 Å | |||||||||
Authors | Orozco-Navarrete, B. / Kaczmarska, Z. / Dupeux, F. / Pott, D. / Diaz Perales, A. / Casanal, A. / Marquez, J.A. / Valpuesta, V. / Merchante, C. | |||||||||
Funding support | Spain, 2items
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Citation | Journal: J.Agric.Food Chem. / Year: 2020 Title: Structural Bases for the Allergenicity of Fra a 1.02 in Strawberry Fruits. Authors: Orozco-Navarrete, B. / Kaczmarska, Z. / Dupeux, F. / Garrido-Arandia, M. / Pott, D. / Perales, A.D. / Casanal, A. / Marquez, J.A. / Valpuesta, V. / Merchante, C. | |||||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 6sta.cif.gz | 72.7 KB | Display | PDBx/mmCIF format |
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PDB format | pdb6sta.ent.gz | 53.7 KB | Display | PDB format |
PDBx/mmJSON format | 6sta.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/st/6sta ftp://data.pdbj.org/pub/pdb/validation_reports/st/6sta | HTTPS FTP |
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-Related structure data
Related structure data | 6st8C 6st9C 6stbC 5amwS S: Starting model for refinement C: citing same article (ref.) |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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2 |
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Unit cell |
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-Components
#1: Protein | Mass: 17617.979 Da / Num. of mol.: 2 / Mutation: E46A, D48A Source method: isolated from a genetically manipulated source Source: (gene. exp.) Fragaria ananassa (strawberry) / Gene: FRAA2 / Production host: Escherichia coli BL21(DE3) (bacteria) / References: UniProt: D0E0C6 #2: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.59 Å3/Da / Density % sol: 52.44 % |
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Crystal grow | Temperature: 293 K / Method: vapor diffusion, sitting drop / Details: 1.6 M sodium citrate tribasic |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N |
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Diffraction source | Source: SYNCHROTRON / Site: ESRF / Beamline: MASSIF-3 / Wavelength: 0.9677 Å |
Detector | Type: DECTRIS EIGER X 4M / Detector: PIXEL / Date: Nov 23, 2017 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.9677 Å / Relative weight: 1 |
Reflection | Resolution: 2.195→55.856 Å / Num. obs: 14982 / % possible obs: 92.2 % / Redundancy: 7.3 % / CC1/2: 0.998 / Rmerge(I) obs: 0.138 / Rpim(I) all: 0.054 / Rrim(I) all: 0.149 / Net I/σ(I): 11 |
Reflection shell | Resolution: 2.195→2.396 Å / Redundancy: 7.7 % / Rmerge(I) obs: 1.466 / Mean I/σ(I) obs: 1.4 / Num. unique obs: 749 / CC1/2: 0.609 / Rpim(I) all: 0.563 / % possible all: 45.1 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: 5AMW Resolution: 2.19→55.86 Å / Cor.coef. Fo:Fc: 0.94 / Cor.coef. Fo:Fc free: 0.903 / SU R Cruickshank DPI: 0.352 / Cross valid method: THROUGHOUT / SU R Blow DPI: 0.361 / SU Rfree Blow DPI: 0.249 / SU Rfree Cruickshank DPI: 0.25
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Displacement parameters | Biso mean: 48.55 Å2
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Refine analyze | Luzzati coordinate error obs: 0.34 Å | ||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.19→55.86 Å
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LS refinement shell | Resolution: 2.19→2.36 Å
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