+Open data
-Basic information
Entry | Database: PDB / ID: 6shc | |||||||||
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Title | Crystal structure of human IRE1 luminal domain Q105C | |||||||||
Components | Serine/threonine-protein kinase/endoribonuclease IRE1 | |||||||||
Keywords | SIGNALING PROTEIN / IRE1 / unfolded protein response / ER stress / protein quality control | |||||||||
Function / homology | Function and homology information peptidyl-serine trans-autophosphorylation / mRNA splicing, via endonucleolytic cleavage and ligation / AIP1-IRE1 complex / Ire1 complex / IRE1alpha activates chaperones / IRE1-TRAF2-ASK1 complex / insulin metabolic process / peptidyl-serine autophosphorylation / IRE1-RACK1-PP2A complex / Hydrolases; Acting on ester bonds; Endoribonucleases producing 5'-phosphomonoesters ...peptidyl-serine trans-autophosphorylation / mRNA splicing, via endonucleolytic cleavage and ligation / AIP1-IRE1 complex / Ire1 complex / IRE1alpha activates chaperones / IRE1-TRAF2-ASK1 complex / insulin metabolic process / peptidyl-serine autophosphorylation / IRE1-RACK1-PP2A complex / Hydrolases; Acting on ester bonds; Endoribonucleases producing 5'-phosphomonoesters / platelet-derived growth factor receptor binding / positive regulation of endoplasmic reticulum unfolded protein response / endothelial cell proliferation / nuclear inner membrane / IRE1-mediated unfolded protein response / mRNA catabolic process / intrinsic apoptotic signaling pathway in response to endoplasmic reticulum stress / regulation of macroautophagy / cellular response to vascular endothelial growth factor stimulus / cellular response to unfolded protein / positive regulation of JUN kinase activity / RNA endonuclease activity / positive regulation of vascular associated smooth muscle cell proliferation / Hsp70 protein binding / response to endoplasmic reticulum stress / positive regulation of RNA splicing / ADP binding / cellular response to glucose stimulus / Hsp90 protein binding / cellular response to hydrogen peroxide / unfolded protein binding / protein autophosphorylation / non-specific serine/threonine protein kinase / protein phosphorylation / protein serine kinase activity / protein serine/threonine kinase activity / endoplasmic reticulum membrane / enzyme binding / magnesium ion binding / endoplasmic reticulum / protein homodimerization activity / mitochondrion / ATP binding / identical protein binding / cytoplasm Similarity search - Function | |||||||||
Biological species | Homo sapiens (human) | |||||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 3.55 Å | |||||||||
Authors | Yan, Y. / Ron, D. | |||||||||
Funding support | United Kingdom, 2items
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Citation | Journal: Elife / Year: 2019 Title: Unstructured regions in IRE1 alpha specify BiP-mediated destabilisation of the luminal domain dimer and repression of the UPR. Authors: Amin-Wetzel, N. / Neidhardt, L. / Yan, Y. / Mayer, M.P. / Ron, D. | |||||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 6shc.cif.gz | 72 KB | Display | PDBx/mmCIF format |
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PDB format | pdb6shc.ent.gz | 42.7 KB | Display | PDB format |
PDBx/mmJSON format | 6shc.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/sh/6shc ftp://data.pdbj.org/pub/pdb/validation_reports/sh/6shc | HTTPS FTP |
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-Related structure data
Related structure data | 2hz6S S: Starting model for refinement |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
#1: Protein | Mass: 41006.367 Da / Num. of mol.: 1 / Mutation: Q105C, C109S, C148S, C332S Source method: isolated from a genetically manipulated source Details: Four mutations has been introduced into the construct: Q105C, C109S, C148S, C332S. Source: (gene. exp.) Homo sapiens (human) / Gene: ERN1, IRE1 / Production host: Escherichia coli BL21(DE3) (bacteria) / Variant (production host): Origami B References: UniProt: O75460, non-specific serine/threonine protein kinase, Hydrolases; Acting on ester bonds; Endoribonucleases producing 5'-phosphomonoesters |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 5.34 Å3/Da / Density % sol: 76.97 % |
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Crystal grow | Temperature: 293 K / Method: vapor diffusion / pH: 7.5 / Details: 9% MPD, 0.1M Hepes pH7.5 |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N |
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Diffraction source | Source: SYNCHROTRON / Site: Diamond / Beamline: I04-1 / Wavelength: 0.916 Å |
Detector | Type: DECTRIS PILATUS 6M-F / Detector: PIXEL / Date: Mar 4, 2018 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.916 Å / Relative weight: 1 |
Reflection | Resolution: 3.55→91.39 Å / Num. obs: 8590 / % possible obs: 100 % / Redundancy: 19.3 % / Biso Wilson estimate: 143.03 Å2 / CC1/2: 1 / Rmerge(I) obs: 0.18 / Rpim(I) all: 0.058 / Rrim(I) all: 0.189 / Net I/σ(I): 11.7 |
Reflection shell | Resolution: 3.55→3.89 Å / Rmerge(I) obs: 2.242 / Mean I/σ(I) obs: 1.5 / Num. unique obs: 1996 / CC1/2: 0.797 / Rpim(I) all: 0.719 / Rrim(I) all: 2.355 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: 2hz6 Resolution: 3.55→54.79 Å / SU ML: 0.6921 / Cross valid method: FREE R-VALUE / σ(F): 1.34 / Phase error: 42.6377 Stereochemistry target values: GeoStd + Monomer Library + CDL v1.2
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL | ||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 126.62 Å2 | ||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 3.55→54.79 Å
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Refine LS restraints |
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LS refinement shell |
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