- PDB-6s8k: Structure, Thermodynamics, and Kinetics of Plinabulin Binding to ... -
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ID or keywords:
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Basic information
Entry
Database: PDB / ID: 6s8k
Title
Structure, Thermodynamics, and Kinetics of Plinabulin Binding to two Tubulin Isotypes
Components
Designed ankyrin repeat protein (DARPIN) D1
Tubulin alpha-1B chain
Tubulin beta-2B chain
Keywords
STRUCTURAL PROTEIN / Tubulin / Microtubules / Microtubule targeting agents / Cancer
Function / homology
Function and homology information
positive regulation of axon guidance / microtubule-based process / Hydrolases; Acting on acid anhydrides; Acting on GTP to facilitate cellular and subcellular movement / structural constituent of cytoskeleton / microtubule cytoskeleton organization / microtubule cytoskeleton / nervous system development / mitotic cell cycle / microtubule / protein heterodimerization activity ...positive regulation of axon guidance / microtubule-based process / Hydrolases; Acting on acid anhydrides; Acting on GTP to facilitate cellular and subcellular movement / structural constituent of cytoskeleton / microtubule cytoskeleton organization / microtubule cytoskeleton / nervous system development / mitotic cell cycle / microtubule / protein heterodimerization activity / GTPase activity / GTP binding / metal ion binding / cytoplasm / cytosol Similarity search - Function
Mass: 48665.027 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Bos taurus (cattle) / References: UniProt: P81947
#2: Protein
Tubulinbeta-2Bchain
Mass: 49999.887 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Bos taurus (cattle) / References: UniProt: Q6B856
#3: Protein
Designedankyrinrepeatprotein (DARPIN) D1
Mass: 16435.645 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) synthetic construct (others) / Production host: Escherichia coli (E. coli)
Mass: 18.015 Da / Num. of mol.: 870 / Source method: isolated from a natural source / Formula: H2O
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Details
Has ligand of interest
Y
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Experimental details
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Experiment
Experiment
Method: X-RAY DIFFRACTION / Number of used crystals: 1
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Sample preparation
Crystal
Density Matthews: 2.46 Å3/Da / Density % sol: 50.03 %
Crystal grow
Temperature: 293 K / Method: vapor diffusion / pH: 5.5 Details: 100 mM Bis-TrisMethane, pH 5.5, supplemented with 200 mM ammonium sulfate and 25% PEG3350
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Data collection
Diffraction
Mean temperature: 100 K / Serial crystal experiment: N