- PDB-6s6h: Crystal structure of the DNA binding domain of the chromosome-par... -
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基本情報
登録情報
データベース: PDB / ID: 6s6h
タイトル
Crystal structure of the DNA binding domain of the chromosome-partitioning protein ParB complexed to the centromeric parS site
要素
Chromosome-partitioning protein ParB
DNA (5'-D(*GP*AP*TP*GP*TP*TP*TP*CP*AP*CP*GP*TP*GP*AP*AP*AP*CP*AP*TP*C)-3')
キーワード
DNA BINDING PROTEIN / chromosome segregation / chromosome maintenance / protein-DNA recognition / evolution / DNA-binding protein
機能・相同性
機能・相同性情報
positive regulation of sporulation resulting in formation of a cellular spore / chromosome segregation / chromosome / DNA binding / cytoplasm 類似検索 - 分子機能
: / ParB/Spo0J, HTH domain / HTH domain found in ParB protein / ParB/RepB/Spo0J partition protein / ParB/Sulfiredoxin domain / ParB/Sulfiredoxin / ParB-like nuclease domain / ParB/Sulfiredoxin superfamily 類似検索 - ドメイン・相同性
DNA / DNA (> 10) / Chromosome-partitioning protein ParB 類似検索 - 構成要素
#241 - 2020年1月 20年の分子を振り返って (Twenty Years of Molecules) 類似性 (1)
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集合体
登録構造単位
A: Chromosome-partitioning protein ParB B: Chromosome-partitioning protein ParB C: DNA (5'-D(*GP*AP*TP*GP*TP*TP*TP*CP*AP*CP*GP*TP*GP*AP*AP*AP*CP*AP*TP*C)-3') D: DNA (5'-D(*GP*AP*TP*GP*TP*TP*TP*CP*AP*CP*GP*TP*GP*AP*AP*AP*CP*AP*TP*C)-3') ヘテロ分子
分子量: 15502.797 Da / 分子数: 2 / 由来タイプ: 組換発現 詳細: The expressed protein comprised residues 126-254 of the wild-type sequence with an additional N-terminal Met residue and a C-terminal nickel affinity tag of sequence KLAAALEHHHHHH from the ...詳細: The expressed protein comprised residues 126-254 of the wild-type sequence with an additional N-terminal Met residue and a C-terminal nickel affinity tag of sequence KLAAALEHHHHHH from the pET21b expression plasmid. 由来: (組換発現) Caulobacter vibrioides NA1000 (バクテリア) 遺伝子: parB, CCNA_03868 / プラスミド: pET21b / 発現宿主: Escherichia coli BL21(DE3) (大腸菌) / Variant (発現宿主): Rosetta Solu / 参照: UniProt: B8GW30
#2: DNA鎖
DNA (5'-D(*GP*AP*TP*GP*TP*TP*TP*CP*AP*CP*GP*TP*GP*AP*AP*AP*CP*AP*TP*C)-3')
分子量: 6132.991 Da / 分子数: 2 / 由来タイプ: 合成 詳細: Chains C and D form a symmetrical DNA duplex which is similar to the parS sequence from Caulobacter vibrioides without 5' phosphates 由来: (合成) Caulobacter vibrioides (バクテリア)
解像度: 2.4→40.12 Å / Cor.coef. Fo:Fc: 0.944 / Cor.coef. Fo:Fc free: 0.942 / SU B: 19.018 / SU ML: 0.21 / SU R Cruickshank DPI: 0.4347 / 交差検証法: THROUGHOUT / σ(F): 0 / ESU R: 0.435 / ESU R Free: 0.243 詳細: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS U VALUES : WITH TLS ADDED