FMN-dependent NADH-azoreductase / oxidoreductase activity, acting on NAD(P)H as acceptor / oxidoreductase activity, acting on other nitrogenous compounds as donors / FMN binding / electron transfer activity Similarity search - Function
Mass: 23553.732 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Bacillus okhensis (bacteria) / Gene: azoR, LQ50_08590 / Production host: Escherichia coli (E. coli) References: UniProt: A0A0B0IKH9, Oxidoreductases; Acting on other nitrogenous compounds as donors
Resolution: 1.8→42.96 Å / Cor.coef. Fo:Fc: 0.955 / Cor.coef. Fo:Fc free: 0.947 / SU B: 3.883 / SU ML: 0.112 / Cross valid method: THROUGHOUT / ESU R: 0.139 / ESU R Free: 0.126 / Stereochemistry target values: MAXIMUM LIKELIHOOD / Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS
Rfactor
Num. reflection
% reflection
Selection details
Rfree
0.23418
2092
4.9 %
RANDOM
Rwork
0.2085
-
-
-
obs
0.20975
40837
99.6 %
-
Solvent computation
Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å / Solvent model: MASK