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- PDB-6jxn: Crystal Structure of Indigo reductase from Bacillus smithii type ... -
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Open data
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Basic information
Entry | Database: PDB / ID: 6jxn | ||||||
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Title | Crystal Structure of Indigo reductase from Bacillus smithii type strain DSM 4216 | ||||||
![]() | FMN-dependent NADH-azoreductase | ||||||
![]() | OXIDOREDUCTASE / Indigo reductase Aizome (indigo dyeing) | ||||||
Function / homology | ![]() Oxidoreductases; Acting on NADH or NADPH; With a quinone or similar compound as acceptor / FMN-dependent NADH-azoreductase / oxidoreductase activity, acting on NAD(P)H as acceptor / oxidoreductase activity, acting on NAD(P)H, quinone or similar compound as acceptor / FMN binding / electron transfer activity Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Yoneda, K. / Sakuraba, H. / Ohshima, T. | ||||||
![]() | ![]() Title: Structural and biochemical characterization of an extremely thermostable FMN-dependent NADH-indigo reductase from Bacillus smithii. Authors: Yoneda, K. / Yoshioka, M. / Sakuraba, H. / Araki, T. / Ohshima, T. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 193.1 KB | Display | ![]() |
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PDB format | ![]() | 151.4 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 2.9 MB | Display | ![]() |
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Full document | ![]() | 2.9 MB | Display | |
Data in XML | ![]() | 42.8 KB | Display | |
Data in CIF | ![]() | 57.7 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 6jxsC ![]() 3w78S S: Starting model for refinement C: citing same article ( |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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2 | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 25570.969 Da / Num. of mol.: 4 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() References: UniProt: G9QLG5, Oxidoreductases; Acting on other nitrogenous compounds as donors #2: Chemical | ChemComp-FMN / #3: Chemical | #4: Chemical | #5: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.39 Å3/Da / Density % sol: 43.71 % |
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Crystal grow | Temperature: 293 K / Method: vapor diffusion, sitting drop / pH: 9.5 / Details: 40% PEG 600, 0.1 M CHES |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: DECTRIS PILATUS 2M / Detector: PIXEL / Date: Nov 8, 2015 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1 Å / Relative weight: 1 |
Reflection | Resolution: 1.97→35.62 Å / Num. obs: 62256 / % possible obs: 99.9 % / Redundancy: 5 % / Biso Wilson estimate: 11.2 Å2 / Rmerge(I) obs: 0.159 / Net I/σ(I): 9.8 |
Reflection shell | Resolution: 1.97→2 Å / Redundancy: 4 % / Rmerge(I) obs: 0.061 / Num. unique obs: 9820 / % possible all: 99.8 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: 3W78 Resolution: 1.97→35.62 Å / Cross valid method: FREE R-VALUE
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Displacement parameters | Biso mean: 22 Å2 | ||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 1.97→35.62 Å
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LS refinement shell | Resolution: 1.97→2 Å /
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