Evidence: gel filtration, gel filtration coupled with multi-angle light scattering
Type
Name
Symmetry operation
Number
identity operation
1_555
x,y,z
1
Buried area
8390 Å2
ΔGint
-89 kcal/mol
Surface area
36780 Å2
Method
PISA
Unit cell
Length a, b, c (Å)
100.350, 101.570, 123.740
Angle α, β, γ (deg.)
90.00, 90.00, 90.00
Int Tables number
19
Space group name H-M
P212121
-
Components
-
Protein , 1 types, 2 molecules AB
#1: Protein
Obtusifoliol14alphademethylase
Mass: 52447.324 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Details: 42 N-terminal residues were replaced with the leading sequence MAKKTSSKGK. 6xHis tag was added at the C-terminus. Source: (gene. exp.) Acanthamoeba castellanii str. Neff (eukaryote) Gene: ACA1_372720 / Plasmid: pCW-LIC / Production host: Escherichia coli (E. coli) / Strain (production host): HMS 174 / References: UniProt: L8GJB3