PDB-6pki: Zebrafish N-acetylglucosamine-1-phosphodiester alpha-N-acetylgluc...

ID or keywords:

Entry

Database: PDB / ID: 6pki

Title

Zebrafish N-acetylglucosamine-1-phosphodiester alpha-N-acetylglucosaminidase (NAGPA) catalytic domain (C56S C230S) in complex with N-acetyl-alpha-D-glucosamine (alpha-GlcNAc) and mannose 6-phosphate (M6P)

Components

N-acetylglucosamine-1-phosphodiester alpha-N-acetylglucosaminidase

Keywords

HYDROLASE / uncovering enzyme / mannose 6-phosphate / glycosidase / n-acetylglucosamine

Function / homology

Function and homology information

Phosphodiester glycosidase / Phosphodiester glycosidase / Teneurin-like EGF domain / Laminin-type EGF domain / Epidermal growth factor-like domain. / EGF-like domain profile. / EGF-like domain signature 1. / EGF-like domain
Similarity search - Domain/homology

Biological species

Danio rerio (zebrafish)

Method

X-RAY DIFFRACTION /

SYNCHROTRON /

MOLECULAR REPLACEMENT / Resolution: 2.293 Å

Authors

Gorelik, A. / Illes, K. / Nagar, B.

Funding support

Canada, 1items

Citation

Journal: Structure / Year: 2020
Title: Crystal Structure of the Mannose-6-Phosphate Uncovering Enzyme.
Authors: Gorelik, A. / Illes, K. / Nagar, B.

History

Deposition	Jun 29, 2019	Deposition site: RCSB / Processing site: RCSB
Revision 1.0	Feb 19, 2020	Provider: repository / Type: Initial release
Revision 1.1	Mar 11, 2020	Group: Database references / Category: citation Item: _citation.country / _citation.journal_abbrev ..._citation.country / _citation.journal_abbrev / _citation.journal_id_ASTM / _citation.journal_id_CSD / _citation.journal_id_ISSN / _citation.pdbx_database_id_DOI / _citation.pdbx_database_id_PubMed / _citation.title / _citation.year
Revision 1.2	Apr 15, 2020	Group: Database references / Category: citation Item: _citation.journal_volume / _citation.page_first / _citation.page_last
Revision 2.0	Jul 29, 2020	Group: Atomic model / Data collection ...Atomic model / Data collection / Derived calculations / Structure summary Category: atom_site / chem_comp ...atom_site / chem_comp / entity / pdbx_chem_comp_identifier / pdbx_entity_nonpoly / struct_conn / struct_site / struct_site_gen Item: _atom_site.auth_atom_id / _atom_site.label_atom_id ..._atom_site.auth_atom_id / _atom_site.label_atom_id / _chem_comp.mon_nstd_flag / _chem_comp.name / _chem_comp.type / _entity.pdbx_description / _pdbx_entity_nonpoly.name / _struct_conn.pdbx_role Description: Carbohydrate remediation / Provider: repository / Type: Remediation
Revision 2.1	Oct 11, 2023	Group: Data collection / Database references ...Data collection / Database references / Refinement description / Structure summary Category: chem_comp / chem_comp_atom ...chem_comp / chem_comp_atom / chem_comp_bond / database_2 / pdbx_initial_refinement_model Item: _chem_comp.pdbx_synonyms / _database_2.pdbx_DOI / _database_2.pdbx_database_accession
Revision 2.2	Nov 20, 2024	Group: Structure summary / Category: pdbx_entry_details / pdbx_modification_feature / Item: _pdbx_entry_details.has_protein_modification

Structure viewer	Molecule: MolmilJmol/JSmol

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Download

PDBx/mmCIF format	6pki.cif.gz	683.9 KB	Display	PDBx/mmCIF format
PDB format	pdb6pki.ent.gz	579.9 KB	Display	PDB format
PDBx/mmJSON format	6pki.json.gz		Tree view	PDBx/mmJSON format
Others	Other downloads

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Validation report

Arichive directory	https://data.pdbj.org/pub/pdb/validation_reports/pk/6pki ftp://data.pdbj.org/pub/pdb/validation_reports/pk/6pki	HTTPS FTP

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Related structure data

Related structure data	6pkgSC 6pkhC 6pkuC 6pkyC 6u11C S: Starting model for refinement C: citing same article (ref.)
Similar structure data	6g3m-1 2gra-6 2pj4-d 4xag-d 1wpr-d 5tnp-2 4kev-2 3fdk-1 6unc-6 6g1j-1 Search similar-shape structures by Omokage search (details) Search similar-shape structures by Omokage search (details) Search similar-shape structures by Omokage search (details) Similarity search - Function & homologyF&H Search Similarity search - HomologyF&H Search

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Links

PDB pages	PDBj / wwPDB / NCBI
Related items in Molecule of the Month	#99 - Mar 2008 Cadherin similarity (1) #76 - Apr 2006 Hemagglutinin similarity (1) #83 - Nov 2006 Fibrin similarity (1) #126 - Jun 2010 Epidermal Growth Factor similarity (1) #75 - Mar 2006 Tissue Factor similarity (1)

Deposited unit

A: N-acetylglucosamine-1-phosphodiester alpha-N-acetylglucosaminidase

B: N-acetylglucosamine-1-phosphodiester alpha-N-acetylglucosaminidase

C: N-acetylglucosamine-1-phosphodiester alpha-N-acetylglucosaminidase

D: N-acetylglucosamine-1-phosphodiester alpha-N-acetylglucosaminidase

hetero molecules

141 kDa, 4 polymers
Search similar-shape structures of this assembly by Omokage search (details)

1

A: N-acetylglucosamine-1-phosphodiester alpha-N-acetylglucosaminidase

B: N-acetylglucosamine-1-phosphodiester alpha-N-acetylglucosaminidase

hetero molecules

defined by author&software
Evidence: gel filtration
70.3 kDa, 2 polymers
Search similar-shape structures of this assembly by Omokage search (details)

2

C: N-acetylglucosamine-1-phosphodiester alpha-N-acetylglucosaminidase

D: N-acetylglucosamine-1-phosphodiester alpha-N-acetylglucosaminidase

hetero molecules

defined by author&software
70.2 kDa, 2 polymers
Search similar-shape structures of this assembly by Omokage search (details)

Unit cell

Length a, b, c (Å)	124.170, 124.170, 276.989
Angle α, β, γ (deg.)	90.00, 90.00, 90.00
Int Tables number	92
Space group name H-M	P4₁2₁2

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Protein , 1 types, 4 molecules ABCD

#1: Protein	N-acetylglucosamine-1-phosphodiester alpha-N-acetylglucosaminidase Mass: 34048.809 Da / Num. of mol.: 4 / Mutation: C75S, C249S Source method: isolated from a genetically manipulated source Source: (gene. exp.) Danio rerio (zebrafish) / Gene: nagpa / Production host: Spodoptera frugiperda (fall armyworm) / References: UniProt: F1QSF9

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Sugars , 3 types, 17 molecules

#2: Sugar

ChemComp-NAG / 2-acetamido-2-deoxy-beta-D-glucopyranose / N-acetyl-beta-D-glucosamine / 2-acetamido-2-deoxy-beta-D-glucose / 2-acetamido-2-deoxy-D-glucose / 2-acetamido-2-deoxy-glucose / N-ACETYL-D-GLUCOSAMINE

Type: D-saccharide, beta linking / Mass: 221.208 Da / Num. of mol.: 8
Source method: isolated from a genetically manipulated source
Formula: C₈H₁₅NO₆

Identifier	Type	Program
DGlcpNAcb	CONDENSED IUPAC CARBOHYDRATE SYMBOL	GMML 1.0
N-acetyl-b-D-glucopyranosamine	COMMON NAME	GMML 1.0
b-D-GlcpNAc	IUPAC CARBOHYDRATE SYMBOL	PDB-CARE 1.0
GlcNAc	SNFG CARBOHYDRATE SYMBOL	GMML 1.0

#3: Sugar

ChemComp-NDG / 2-acetamido-2-deoxy-alpha-D-glucopyranose / N-acetyl-alpha-D-glucosamine / 2-acetamido-2-deoxy-alpha-D-glucose / 2-acetamido-2-deoxy-D-glucose / 2-acetamido-2-deoxy-glucose / 2-(ACETYLAMINO)-2-DEOXY-A-D-GLUCOPYRANOSE

Type: D-saccharide, alpha linking / Mass: 221.208 Da / Num. of mol.: 4
Source method: isolated from a genetically manipulated source
Formula: C₈H₁₅NO₆ / Feature type: SUBJECT OF INVESTIGATION

Identifier	Type	Program
DGlcpNAca	CONDENSED IUPAC CARBOHYDRATE SYMBOL	GMML 1.0
N-acetyl-a-D-glucopyranosamine	COMMON NAME	GMML 1.0
a-D-GlcpNAc	IUPAC CARBOHYDRATE SYMBOL	PDB-CARE 1.0
GlcNAc	SNFG CARBOHYDRATE SYMBOL	GMML 1.0

#4: Sugar

ChemComp-M6P / 6-O-phosphono-alpha-D-mannopyranose / ALPHA-D-MANNOSE-6-PHOSPHATE / 6-O-phosphono-alpha-D-mannose / 6-O-phosphono-D-mannose / 6-O-phosphono-mannose

Type: D-saccharide, alpha linking / Mass: 260.136 Da / Num. of mol.: 5 / Source method: obtained synthetically / Formula: C₆H₁₃O₉P / Feature type: SUBJECT OF INVESTIGATION

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Non-polymers , 3 types, 630 molecules

#5: Chemical	ChemComp-1PE / PENTAETHYLENE GLYCOL / PEG400 Mass: 238.278 Da / Num. of mol.: 1 / Source method: obtained synthetically / Formula: C₁₀H₂₂O₆ / Comment: precipitant*YM
#6: Chemical	ChemComp-CL / CHLORIDE ION Mass: 35.453 Da / Num. of mol.: 4 / Source method: obtained synthetically / Formula: Cl
#7: Water	ChemComp-HOH / water Mass: 18.015 Da / Num. of mol.: 625 / Source method: isolated from a natural source / Formula: H₂O

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Details

Has ligand of interest	Y
Has protein modification	Y

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Experiment

Experiment	Method: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

Crystal	Density Matthews: 3.92 Å³/Da / Density % sol: 68.62 %
Crystal grow	Temperature: 295 K / Method: vapor diffusion, hanging drop / pH: 6.5 Details: 50% PEG 200, 0.1M HEPES pH 6.5, soaked in 50mM N-acetylgucosamine and 50 mM mannose 6-phosphate without HEPES

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Data collection

Diffraction	Mean temperature: 100 K / Serial crystal experiment: N
Diffraction source	Source: SYNCHROTRON / Site: ALS / Beamline: 5.0.2 / Wavelength: 1 Å
Detector	Type: DECTRIS PILATUS3 6M / Detector: PIXEL / Date: May 26, 2019
Radiation	Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelength	Wavelength: 1 Å / Relative weight: 1
Reflection	Resolution: 2.293→50 Å / Num. obs: 97753 / % possible obs: 100 % / Redundancy: 28.5 % / Net I/σ(I): 21.9
Reflection shell	Resolution: 2.293→2.38 Å / Num. unique obs: 9602

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Processing

Software

Name	Version	Classification
PHENIX	(1.15_3459: ???)	refinement
HKL-2000		data reduction
HKL-2000		data scaling
PHASER		phasing

Refinement

Method to determine structure:

MOLECULAR REPLACEMENT
Starting model: 6PKG

6pkg

Resolution: 2.293→22.661 Å / SU ML: 0.22 / Cross valid method: FREE R-VALUE / σ(F): 1.33 / Phase error: 21.92

	R_factor	Num. reflection	% reflection
R_free	0.204	1997	2.56 %
R_work	0.1698	-	-
obs	0.1706	78149	80.12 %

Solvent computation

Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å

Refinement step

Cycle: LAST / Resolution: 2.293→22.661 Å

	Protein	Nucleic acid	Ligand	Solvent	Total
Num. atoms	9181	0	272	625	10078

Refine LS restraints

Refine-ID	Type	Dev ideal	Number
X-RAY DIFFRACTION	f_bond_d	0.003	9649
X-RAY DIFFRACTION	f_angle_d	0.581	13090
X-RAY DIFFRACTION	f_dihedral_angle_d	10.092	5648
X-RAY DIFFRACTION	f_chiral_restr	0.046	1478
X-RAY DIFFRACTION	f_plane_restr	0.003	1704

LS refinement shell

Resolution (Å)	R_factor R_free	Num. reflection R_free	R_factor R_work	Num. reflection R_work	Refine-ID	% reflection obs (%)
2.2927-2.35	0.2057	48	0.2048	1823	X-RAY DIFFRACTION	27
2.35-2.4135	0.2692	57	0.195	2212	X-RAY DIFFRACTION	33
2.4135-2.4844	0.2375	73	0.2121	2762	X-RAY DIFFRACTION	41
2.4844-2.5645	0.246	94	0.2122	3582	X-RAY DIFFRACTION	54
2.5645-2.656	0.2863	122	0.2151	4634	X-RAY DIFFRACTION	69
2.656-2.7622	0.2658	165	0.2108	6335	X-RAY DIFFRACTION	94
2.7622-2.8877	0.2218	177	0.1957	6718	X-RAY DIFFRACTION	100
2.8877-3.0396	0.2439	177	0.1819	6747	X-RAY DIFFRACTION	100
3.0396-3.2295	0.2125	176	0.1734	6741	X-RAY DIFFRACTION	100
3.2295-3.478	0.1955	179	0.1541	6781	X-RAY DIFFRACTION	100
3.478-3.8265	0.1779	177	0.1428	6812	X-RAY DIFFRACTION	100
3.8265-4.3768	0.1604	180	0.1361	6860	X-RAY DIFFRACTION	100
4.3768-5.5012	0.1636	182	0.1373	6921	X-RAY DIFFRACTION	100
5.5012-22.6623	0.2425	190	0.2149	7224	X-RAY DIFFRACTION	100

Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

ID	L11 (°²)	L12 (°²)	L13 (°²)	L22 (°²)	L23 (°²)	L33 (°²)	S11 (Å °)	S12 (Å °)	S13 (Å °)	S21 (Å °)	S22 (Å °)	S23 (Å °)	S31 (Å °)	S32 (Å °)	S33 (Å °)	T11 (Å²)	T12 (Å²)	T13 (Å²)	T22 (Å²)	T23 (Å²)	T33 (Å²)	Origin x (Å)	Origin y (Å)	Origin z (Å)
1	2.2122	-0.4745	0.022	4.5402	0.2814	3.6864	0.0493	0.2075	0.019	-0.1901	0.0142	-0.0421	0.2236	0.0812	-0.0858	0.1541	-0.0075	-0.045	0.2141	0.0323	0.1296	39.2852	23.0901	-3.593
2	3.5829	-3.4485	4.2956	4.2038	-4.5617	5.6239	-0.298	0.2735	0.3253	0.2711	-0.0579	-0.343	-0.4784	0.6226	0.2607	0.2811	-0.029	-0.0952	0.297	0.0213	0.4035	52.0096	26.1126	17.1978
3	2.2636	0.5586	-0.7311	2.1099	-0.0792	2.7562	0.0129	0.1443	0.0875	0.0087	0.0511	-0.0592	0.2035	0.0254	-0.0673	0.3272	0.0436	-0.0882	0.1783	0.0392	0.2489	42.7104	10.0748	16.804
4	2.7156	-3.1454	-0.618	5.1743	2.1336	4.5459	0.0499	-0.0727	-0.2518	0.3086	-0.0343	-0.3314	0.6672	0.6398	-0.0783	0.4008	0.1031	-0.0286	0.2549	0.0087	0.3781	52.6056	3.5761	16.842
5	2.7914	-0.7166	-2.8083	7.6812	-0.6119	4.2949	-0.1889	0.0707	-0.4884	-0.5008	0.0086	0.1029	0.6368	0.077	0.0744	0.4195	0.07	-0.0677	0.2912	-0.0509	0.2823	45.032	6.1466	-0.2202
6	2.1016	-0.3148	0.4299	2.4027	1.0062	4.8417	-0.1635	0.1935	0.1435	-0.043	0.0289	-0.254	0.0423	0.5143	0.0884	0.2056	-0.0168	0.0299	0.239	0.0402	0.2635	53.0957	18.9574	4.9895
7	3.2276	-1.2667	-0.3891	2.1091	-0.3179	1.8397	-0.0902	0.4438	0.3499	-0.1073	0.0145	-0.0352	-0.0066	0.0837	0.0151	0.1966	-0.0025	-0.0348	0.1626	0.0229	0.1537	43.9028	22.9481	2.7729
8	3.263	1.3962	-0.3415	3.6745	0.3878	1.2276	-0.1355	0.1911	-0.0446	0.0028	0.098	0.0036	0.0773	-0.0837	0.1183	0.1881	0.019	-0.0254	0.0775	0.0006	0.1799	37.1923	16.0466	11.9848
9	1.4756	0.1225	-0.4839	1.6239	-0.8641	0.585	-0.0222	0.0464	-0.0308	0.042	0.0475	0.0443	0.2736	-0.1095	-0.0346	0.3307	0.0005	-0.0645	0.1286	-0.0049	0.197	36.458	8.9	15.3668
10	2.528	1.0123	-0.8361	4.0155	-0.8248	1.8008	0.0803	-0.0879	-0.2894	0.1037	-0.1356	-0.1435	0.5357	0.194	0.1444	0.4098	0.1268	-0.0067	0.1679	0.002	0.2054	46.6197	2.0135	14.7919
11	1.7677	0.1859	0.0558	2.2692	-0.3805	0.9291	0.2427	-0.1036	-0.1686	-0.1855	-0.0908	-0.0743	0.1604	-0.105	-0.0228	0.5718	-0.0142	-0.0442	0.154	0.0208	0.3098	29.0156	-20.735	43.392
12	0.4269	0.2786	0.4466	2.1785	-0.7477	2.9317	0.0076	-0.0313	0.0222	-0.0183	-0.0592	-0.1431	0.1593	0.0536	0.0052	0.3167	0.0516	-0.012	0.1541	0.0001	0.312	38.5954	-6.9735	35.5596
13	0.9309	0.7131	-0.2958	1.9664	0.052	0.1593	0.0355	-0.0503	-0.1621	-0.0976	-0.0737	0.018	0.4508	0.0795	0.0422	0.4772	0.0756	-0.0623	0.1004	0.0294	0.2314	35.954	-20.7563	44.7691
14	2.7168	-0.6441	0.0923	2.938	0.3314	1.5441	-0.0655	-0.0812	-0.0316	-0.1403	0.0929	0.182	0.3423	-0.2325	0.144	0.3329	-0.0033	-0.0619	0.0865	0.0289	0.2174	25.5993	-12.1768	37.7296
15	1.1637	1.0235	-0.1968	1.479	-0.7219	1.3703	-0.0241	0.0473	0.0172	-0.163	0.0288	0.0593	0.1392	0.0242	-0.0223	0.344	0.0317	-0.0472	0.1015	0.0015	0.2195	34.1931	-3.8411	32.6666
16	1.1608	0.1777	-0.405	1.7897	1.0382	3.8219	0.0538	-0.245	0.1529	0.6518	-0.0902	0.2396	-0.0286	-0.1777	0.0424	0.6507	0.0318	0.0193	0.2342	-0.0374	0.3802	45.3935	25.5441	60.0899
17	2.6559	0.516	-0.1513	0.8376	0.1875	2.6638	-0.0202	-0.0457	0.0102	0.2603	0.2085	0.0797	-0.0787	0.4303	-0.165	0.5769	0.0611	-0.1042	0.3233	-0.0915	0.3041	59.4733	22.2419	47.8355
18	2.5299	-0.1768	-0.6786	1.8991	-0.7278	2.8953	0.2122	-0.3087	-0.0773	0.7043	0.0061	-0.4435	0.7091	0.7588	-0.132	0.7978	0.1423	-0.2036	0.4621	-0.0487	0.4244	62.3628	14.7114	56.0412
19	1.6725	0.4281	-0.4542	2.3566	0.2112	1.7396	0.0745	-0.1922	0.0021	0.8115	-0.1037	0.1148	0.1124	-0.0557	0.0224	0.7106	0.0263	-0.0308	0.2109	-0.0151	0.2963	45.9684	18.6286	59.4839
20	1.8059	0.2522	-0.0486	1.3508	1.5616	2.286	0.1238	-0.1938	0.2728	0.3605	0.0631	-0.0944	-0.3181	0.4463	-0.1271	0.6942	-0.0355	-0.1175	0.2028	-0.0817	0.3185	56.5658	31.5183	55.1722
21	1.7022	0.7594	-0.256	2.2033	0.2965	2.2604	0.0783	0.0516	0.1211	0.2562	0.0771	-0.1433	-0.1861	0.731	-0.1255	0.557	-0.0132	-0.0997	0.3635	-0.0525	0.2133	61.481	26.3248	48.8072
22	4.6525	1.4301	-0.9698	2.3265	-2.1544	2.0282	0.0036	0.215	-0.2573	0.2966	0.1806	-0.3519	0.2393	0.7803	-0.1561	0.6125	0.2091	-0.1589	0.5652	-0.1022	0.2738	65.7132	16.0556	49.9025
23	4.2955	-0.4805	-0.4761	2.2103	0.0942	1.713	0.1065	0.2203	0.6867	-0.0613	0.0102	-0.5249	-0.1981	0.7021	-0.2163	0.7939	-0.27	-0.0181	1.7266	-0.0757	0.7828	91.9811	31.4805	14.5369
24	4.3322	0.3307	-0.7812	0.7836	0.8918	1.336	-0.1449	-0.1383	-0.4755	0.3362	0.085	-0.5505	0.4033	0.9256	0.1015	0.5636	0.3308	-0.2153	1.3257	-0.1291	0.6254	95.3326	13.6344	31.4451
25	2.3939	-0.2861	-0.24	2.834	0.7357	3.0703	0.3653	-0.2098	0.5924	0.4138	-0.3156	-0.5992	-0.3762	1.047	-0.0768	0.6241	-0.1744	-0.1195	0.9386	-0.1833	0.5655	80.2567	30.0371	41.4061
26	3.1573	-0.1765	-0.274	1.1986	0.4308	0.3565	-0.135	0.2257	-0.1871	0.1238	0.1191	-0.0434	0.2397	0.9055	0.0164	0.4562	0.1288	-0.1267	0.8202	-0.1193	0.3875	80.1736	15.1038	21.4779
27	0.657	-0.0225	-0.2249	0.3437	-0.2257	0.3039	0.1849	-0.1389	0.2732	0.2006	0.0337	-0.2545	-0.1196	1.0603	0.5734	0.4343	-0.0739	-0.186	1.0165	-0.1779	0.4421	82.5763	25.4633	28.2292

Refinement TLS group

ID	Refine-ID	Refine TLS-ID	Selection details
1	X-RAY DIFFRACTION	1	chain 'A' and (resid 36 through 61 )
2	X-RAY DIFFRACTION	2	chain 'A' and (resid 62 through 79 )
3	X-RAY DIFFRACTION	3	chain 'A' and (resid 80 through 131 )
4	X-RAY DIFFRACTION	4	chain 'A' and (resid 132 through 146 )
5	X-RAY DIFFRACTION	5	chain 'A' and (resid 147 through 160 )
6	X-RAY DIFFRACTION	6	chain 'A' and (resid 161 through 190 )
7	X-RAY DIFFRACTION	7	chain 'A' and (resid 191 through 235 )
8	X-RAY DIFFRACTION	8	chain 'A' and (resid 236 through 265 )
9	X-RAY DIFFRACTION	9	chain 'A' and (resid 266 through 319 )
10	X-RAY DIFFRACTION	10	chain 'A' and (resid 320 through 336 )
11	X-RAY DIFFRACTION	11	chain 'B' and (resid 36 through 79 )
12	X-RAY DIFFRACTION	12	chain 'B' and (resid 80 through 160 )
13	X-RAY DIFFRACTION	13	chain 'B' and (resid 161 through 221 )
14	X-RAY DIFFRACTION	14	chain 'B' and (resid 222 through 265 )
15	X-RAY DIFFRACTION	15	chain 'B' and (resid 266 through 336 )
16	X-RAY DIFFRACTION	16	chain 'C' and (resid 36 through 79 )
17	X-RAY DIFFRACTION	17	chain 'C' and (resid 80 through 131 )
18	X-RAY DIFFRACTION	18	chain 'C' and (resid 132 through 160 )
19	X-RAY DIFFRACTION	19	chain 'C' and (resid 161 through 221 )
20	X-RAY DIFFRACTION	20	chain 'C' and (resid 222 through 275 )
21	X-RAY DIFFRACTION	21	chain 'C' and (resid 276 through 319 )
22	X-RAY DIFFRACTION	22	chain 'C' and (resid 320 through 336 )
23	X-RAY DIFFRACTION	23	chain 'D' and (resid 36 through 61 )
24	X-RAY DIFFRACTION	24	chain 'D' and (resid 62 through 79 )
25	X-RAY DIFFRACTION	25	chain 'D' and (resid 80 through 106 )
26	X-RAY DIFFRACTION	26	chain 'D' and (resid 107 through 190 )
27	X-RAY DIFFRACTION	27	chain 'D' and (resid 191 through 336 )

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