glycolytic process through fructose-1-phosphate / Fructose catabolism / fructose catabolic process to hydroxyacetone phosphate and glyceraldehyde-3-phosphate / ketohexokinase / ketohexokinase activity / regulation of glycogen metabolic process / response to sucrose / response to fructose / fructose metabolic process / response to zinc ion ...glycolytic process through fructose-1-phosphate / Fructose catabolism / fructose catabolic process to hydroxyacetone phosphate and glyceraldehyde-3-phosphate / ketohexokinase / ketohexokinase activity / regulation of glycogen metabolic process / response to sucrose / response to fructose / fructose metabolic process / response to zinc ion / response to glucose / response to insulin / ATP binding / nucleus / cytoplasm Similarity search - Function
Mass: 18.015 Da / Num. of mol.: 389 / Source method: isolated from a natural source / Formula: H2O
Has ligand of interest
Y
-
Experimental details
-
Experiment
Experiment
Method: X-RAY DIFFRACTION / Number of used crystals: 1
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Sample preparation
Crystal
Density Matthews: 1.79 Å3/Da / Density % sol: 31.32 %
Crystal grow
Temperature: 290.15 K / Method: vapor diffusion, hanging drop / pH: 7 Details: 1.2 M Ammonium Citrate Tribasic, 20% glycerol, 1.3 M KNO3, 100 mM MgCl2, 220 mM fructose, and 52 mM ADP
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Data collection
Diffraction
Mean temperature: 100 K / Serial crystal experiment: N
Diffraction source
Source: ROTATING ANODE / Type: BRUKER AXS MICROSTAR / Wavelength: 1.54 Å
Detector
Type: Bruker Platinum 135 / Detector: CCD / Date: Dec 22, 2016
Radiation
Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelength
Wavelength: 1.54 Å / Relative weight: 1
Reflection
Resolution: 1.79→37.96 Å / Num. obs: 23632 / % possible obs: 97 % / Redundancy: 1.88 % / Biso Wilson estimate: 9.33 Å2 / CC1/2: 0.99 / Rmerge(I) obs: 0.14 / Rrim(I) all: 0.15 / Net I/σ(I): 14.3
Reflection shell
Resolution: 1.79→1.86 Å / Rmerge(I) obs: 0.3 / Mean I/σ(I) obs: 6.4 / Num. unique obs: 2058 / CC1/2: 0.95 / % possible all: 74
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Processing
Software
Name
Version
Classification
PHENIX
1.13_2998
refinement
PHENIX
1.13_2998
refinement
SADABS
datascaling
PDB_EXTRACT
3.25
dataextraction
SAINT
datareduction
PHENIX
1.10.1_2155
phasing
Refinement
Method to determine structure: MOLECULAR REPLACEMENT Starting model: 3Q92
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