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- PDB-6o41: Crystal structure of the unbound PGZL1 germline Fab fragment (PGZ... -

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Basic information

Entry
Database: PDB / ID: 6o41
TitleCrystal structure of the unbound PGZL1 germline Fab fragment (PGZL1_gVmDmJ)
Components
  • Immunoglobulin G-binding protein G (DOMAIN III)
  • germline PGZL1_gVmDmJ heavy chain
  • germline PGZL1_gVmDmJ light chain
KeywordsIMMUNE SYSTEM / germline PGZL1 ANTI HIV-1 / GP41 MPER / MEMBRANE LIPIDS / BROADLY NEUTRALISING HIV-1 ANTIBODY
Function / homology
Function and homology information


IgG binding / extracellular region
Similarity search - Function
IgG-binding B / B domain / Ubiquitin-like (UB roll) - #10 / M protein-type anchor domain / GA-like domain / GA-like domain / Immunoglobulin/albumin-binding domain superfamily / YSIRK Gram-positive signal peptide / LPXTG cell wall anchor motif / Gram-positive cocci surface proteins LPxTG motif profile. ...IgG-binding B / B domain / Ubiquitin-like (UB roll) - #10 / M protein-type anchor domain / GA-like domain / GA-like domain / Immunoglobulin/albumin-binding domain superfamily / YSIRK Gram-positive signal peptide / LPXTG cell wall anchor motif / Gram-positive cocci surface proteins LPxTG motif profile. / LPXTG cell wall anchor domain / Ubiquitin-like (UB roll) / Roll / Immunoglobulins / Immunoglobulin-like / Sandwich / Mainly Beta / Alpha Beta
Similarity search - Domain/homology
Immunoglobulin G-binding protein G
Similarity search - Component
Biological speciesHomo sapiens (human)
Streptococcus sp. group G (bacteria)
MethodX-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2.465 Å
AuthorsIrimia, A. / Wilson, I.A.
Funding support United States, 1items
OrganizationGrant numberCountry
National Institutes of Health/National Institute Of Allergy and Infectious Diseases (NIH/NIAID)UM1 AI100663 United States
CitationJournal: Nat Commun / Year: 2019
Title: An MPER antibody neutralizes HIV-1 using germline features shared among donors.
Authors: Lei Zhang / Adriana Irimia / Lingling He / Elise Landais / Kimmo Rantalainen / Daniel P Leaman / Thomas Vollbrecht / Armando Stano / Daniel I Sands / Arthur S Kim / / Pascal Poignard / ...Authors: Lei Zhang / Adriana Irimia / Lingling He / Elise Landais / Kimmo Rantalainen / Daniel P Leaman / Thomas Vollbrecht / Armando Stano / Daniel I Sands / Arthur S Kim / / Pascal Poignard / Dennis R Burton / Ben Murrell / Andrew B Ward / Jiang Zhu / Ian A Wilson / Michael B Zwick /
Abstract: The membrane-proximal external region (MPER) of HIV-1 envelope glycoprotein (Env) can be targeted by neutralizing antibodies of exceptional breadth. MPER antibodies usually have long, hydrophobic ...The membrane-proximal external region (MPER) of HIV-1 envelope glycoprotein (Env) can be targeted by neutralizing antibodies of exceptional breadth. MPER antibodies usually have long, hydrophobic CDRH3s, lack activity as inferred germline precursors, are often from the minor IgG3 subclass, and some are polyreactive, such as 4E10. Here we describe an MPER broadly neutralizing antibody from the major IgG1 subclass, PGZL1, which shares germline V/D-region genes with 4E10, has a shorter CDRH3, and is less polyreactive. A recombinant sublineage variant pan-neutralizes a 130-isolate panel at 1.4 μg/ml (IC). Notably, a germline revertant with mature CDR3s neutralizes 12% of viruses and still binds MPER after DJ reversion. Crystal structures of lipid-bound PGZL1 variants and cryo-EM reconstruction of an Env-PGZL1 complex reveal how these antibodies recognize MPER and viral membrane. Discovery of common genetic and structural elements among MPER antibodies from different patients suggests that such antibodies could be elicited using carefully designed immunogens.
History
DepositionFeb 27, 2019Deposition site: RCSB / Processing site: RCSB
Revision 1.0Dec 4, 2019Provider: repository / Type: Initial release
Revision 1.1Dec 11, 2019Group: Database references / Category: citation / citation_author
Item: _citation.journal_volume / _citation.page_first ..._citation.journal_volume / _citation.page_first / _citation.page_last / _citation.pdbx_database_id_DOI / _citation.pdbx_database_id_PubMed / _citation.title / _citation_author.identifier_ORCID
Revision 1.2Oct 11, 2023Group: Data collection / Database references / Refinement description
Category: chem_comp_atom / chem_comp_bond ...chem_comp_atom / chem_comp_bond / database_2 / pdbx_initial_refinement_model
Item: _database_2.pdbx_DOI / _database_2.pdbx_database_accession
Revision 1.3Nov 20, 2024Group: Structure summary / Category: pdbx_entry_details / pdbx_modification_feature

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
L: germline PGZL1_gVmDmJ light chain
H: germline PGZL1_gVmDmJ heavy chain
A: germline PGZL1_gVmDmJ light chain
B: germline PGZL1_gVmDmJ heavy chain
C: germline PGZL1_gVmDmJ light chain
D: germline PGZL1_gVmDmJ heavy chain
M: Immunoglobulin G-binding protein G (DOMAIN III)
N: Immunoglobulin G-binding protein G (DOMAIN III)
O: Immunoglobulin G-binding protein G (DOMAIN III)
hetero molecules


Theoretical massNumber of molelcules
Total (without water)165,36622
Polymers164,1699
Non-polymers1,19713
Water7,134396
1
L: germline PGZL1_gVmDmJ light chain
H: germline PGZL1_gVmDmJ heavy chain
M: Immunoglobulin G-binding protein G (DOMAIN III)
hetero molecules


Theoretical massNumber of molelcules
Total (without water)55,36810
Polymers54,7233
Non-polymers6457
Water543
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
2
A: germline PGZL1_gVmDmJ light chain
B: germline PGZL1_gVmDmJ heavy chain
O: Immunoglobulin G-binding protein G (DOMAIN III)
hetero molecules


Theoretical massNumber of molelcules
Total (without water)54,9996
Polymers54,7233
Non-polymers2763
Water543
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
3
C: germline PGZL1_gVmDmJ light chain
D: germline PGZL1_gVmDmJ heavy chain
N: Immunoglobulin G-binding protein G (DOMAIN III)
hetero molecules


Theoretical massNumber of molelcules
Total (without water)54,9996
Polymers54,7233
Non-polymers2763
Water543
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Unit cell
Length a, b, c (Å)78.840, 107.490, 131.960
Angle α, β, γ (deg.)90.00, 94.61, 90.00
Int Tables number4
Space group name H-MP1211

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Components

#1: Antibody germline PGZL1_gVmDmJ light chain


Mass: 23434.900 Da / Num. of mol.: 3
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Cell line (production host): Expi 293F / Production host: Homo sapiens (human)
#2: Antibody germline PGZL1_gVmDmJ heavy chain


Mass: 23939.955 Da / Num. of mol.: 3
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Cell line (production host): Expi 293F / Production host: Homo sapiens (human)
#3: Antibody Immunoglobulin G-binding protein G (DOMAIN III) / IgG-binding protein G


Mass: 7348.088 Da / Num. of mol.: 3
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Streptococcus sp. group G (bacteria) / Gene: spg / Plasmid: PET26B / Production host: Escherichia coli (E. coli) / References: UniProt: P19909
#4: Chemical
ChemComp-GOL / GLYCEROL / GLYCERIN / PROPANE-1,2,3-TRIOL


Mass: 92.094 Da / Num. of mol.: 13 / Source method: obtained synthetically / Formula: C3H8O3
#5: Water ChemComp-HOH / water


Mass: 18.015 Da / Num. of mol.: 396 / Source method: isolated from a natural source / Formula: H2O
Has protein modificationY

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Experimental details

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Experiment

ExperimentMethod: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

CrystalDensity Matthews: 3.48 Å3/Da / Density % sol: 64.43 %
Crystal growTemperature: 293.15 K / Method: vapor diffusion, sitting drop / Details: 20% PEG3350, 0.2 M lithium citrate

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Data collection

DiffractionMean temperature: 100 K / Serial crystal experiment: N
Diffraction sourceSource: SYNCHROTRON / Site: SSRL / Beamline: BL12-2 / Wavelength: 0.97946 Å
DetectorType: DECTRIS PILATUS 6M / Detector: PIXEL / Date: Jul 21, 2018
RadiationProtocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelengthWavelength: 0.97946 Å / Relative weight: 1
ReflectionResolution: 2.465→39.67 Å / Num. obs: 77485 / % possible obs: 97.7 % / Redundancy: 3.5 % / Biso Wilson estimate: 50 Å2 / CC1/2: 0.995 / Rpim(I) all: 0.062 / Rsym value: 0.099 / Net I/σ(I): 9.2
Reflection shellResolution: 2.465→2.53 Å / Redundancy: 3.4 % / Num. unique obs: 5460 / CC1/2: 0.526 / Rpim(I) all: 0.597 / Rsym value: 0.947 / % possible all: 94

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Processing

Software
NameVersionClassification
PHENIX(1.12_2829: ???)refinement
XDS20180126data reduction
XSCALE20180126data scaling
PHASER2.8.2phasing
RefinementMethod to determine structure: MOLECULAR REPLACEMENT
Starting model: 6O3D

6o3d


Resolution: 2.465→39.67 Å / SU ML: 0.35 / Cross valid method: FREE R-VALUE / σ(F): 1.37 / Phase error: 23.66
RfactorNum. reflection% reflection
Rfree0.2187 3869 5 %
Rwork0.169 --
obs0.1714 77455 97.76 %
Solvent computationShrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å
Displacement parametersBiso mean: 52 Å2
Refinement stepCycle: LAST / Resolution: 2.465→39.67 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms11193 0 78 396 11667
Refine LS restraints
Refine-IDTypeDev idealNumber
X-RAY DIFFRACTIONf_bond_d0.00511613
X-RAY DIFFRACTIONf_angle_d0.8215791
X-RAY DIFFRACTIONf_dihedral_angle_d16.3696982
X-RAY DIFFRACTIONf_chiral_restr0.051784
X-RAY DIFFRACTIONf_plane_restr0.0052014
LS refinement shell
Resolution (Å)Rfactor RfreeNum. reflection RfreeRfactor RworkNum. reflection RworkRefine-ID% reflection obs (%)
2.465-2.49510.38921260.29362363X-RAY DIFFRACTION89
2.4951-2.52660.36561210.28872645X-RAY DIFFRACTION98
2.5266-2.55990.30121440.27322631X-RAY DIFFRACTION98
2.5599-2.59490.28951360.2652550X-RAY DIFFRACTION96
2.5949-2.6320.33561240.25732503X-RAY DIFFRACTION93
2.632-2.67130.32641480.25522511X-RAY DIFFRACTION95
2.6713-2.7130.29931290.24832690X-RAY DIFFRACTION99
2.713-2.75750.28611450.23242643X-RAY DIFFRACTION99
2.7575-2.8050.28781360.22782680X-RAY DIFFRACTION99
2.805-2.8560.25221450.22872649X-RAY DIFFRACTION99
2.856-2.91090.28251290.22522690X-RAY DIFFRACTION99
2.9109-2.97030.28491420.2112633X-RAY DIFFRACTION99
2.9703-3.03490.27431500.20712679X-RAY DIFFRACTION99
3.0349-3.10550.27151320.19992639X-RAY DIFFRACTION99
3.1055-3.18310.2421410.18942642X-RAY DIFFRACTION99
3.1831-3.26910.24881390.18662660X-RAY DIFFRACTION99
3.2691-3.36530.23711360.18142615X-RAY DIFFRACTION98
3.3653-3.47380.23491420.18122554X-RAY DIFFRACTION95
3.4738-3.59790.21691410.16422655X-RAY DIFFRACTION99
3.5979-3.74190.22551420.16212670X-RAY DIFFRACTION99
3.7419-3.9120.22791350.15212664X-RAY DIFFRACTION99
3.912-4.11810.19651460.14542647X-RAY DIFFRACTION99
4.1181-4.37580.17291410.11942666X-RAY DIFFRACTION99
4.3758-4.71310.13671320.10562631X-RAY DIFFRACTION98
4.7131-5.18650.1331460.11342579X-RAY DIFFRACTION96
5.1865-5.93490.21380.13782698X-RAY DIFFRACTION99
5.9349-7.46910.20461420.16812682X-RAY DIFFRACTION99
7.4691-39.67480.18021410.1542717X-RAY DIFFRACTION97
Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

IDL11 (°2)L12 (°2)L13 (°2)L22 (°2)L23 (°2)L33 (°2)S11 (Å °)S12 (Å °)S13 (Å °)S21 (Å °)S22 (Å °)S23 (Å °)S31 (Å °)S32 (Å °)S33 (Å °)T11 (Å2)T12 (Å2)T13 (Å2)T22 (Å2)T23 (Å2)T33 (Å2)Origin x (Å)Origin y (Å)Origin z (Å)
14.02440.1640.23412.31240.55973.59480.04360.075-0.0693-0.1362-0.0038-0.08920.1626-0.01260.00320.3704-0.04540.00790.3003-0.05750.3481-30.6731-0.1118-8.9355
21.5003-0.0495-0.64123.6641.66742.64-0.17880.1034-0.24050.1042-0.01350.13010.4448-0.16470.17250.2607-0.0126-0.01950.32840.05530.3236-30.41024.2506-0.5881
31.62580.8034-0.5366.01171.15640.52750.1648-0.07290.1304-0.1458-0.2220.2607-0.182-0.29750.08720.41540.03690.00490.47570.00740.3051-36.886926.58719.301
43.16280.86390.26596.34391.34682.0926-0.1046-0.19150.36230.1325-0.01940.3302-0.1028-0.25570.110.32910.0516-0.01720.3915-0.01460.2654-37.521727.322813.8901
53.67110.2167-0.63474.20091.0084.8194-0.05460.1244-0.1743-0.47150.1894-0.4412-0.04030.3839-0.09280.3498-0.00860.07340.416-0.04410.4192-8.56348.4295-9.825
63.3936-0.07660.02213.881-0.63163.7520.15820.1786-0.73370.1933-0.1836-0.40850.82880.3028-0.27110.36750.06140.07510.4241-0.06220.4289-13.5587-1.2428-7.9377
72.90560.0356-1.20324.76611.03065.46570.664-0.43610.30160.2599-0.18220.2659-0.7715-0.0108-0.08670.35090.064-0.03560.3193-0.03880.3941-22.391626.724217.3769
82.15861.1842-1.91562.33560.28744.38040.0021-0.0829-0.120.22710.0175-0.0743-0.0547-0.1114-0.04630.33010.0179-0.01580.33110.00790.3527-22.475320.601119.86
91.6916-0.61990.87182.5779-0.59422.62390.0737-0.2020.2330.37360.11540.1154-0.33560.2782-0.170.5187-0.1079-0.02760.4322-0.02850.415-36.27636.291655.9366
105.7637-1.07621.2296.3147-1.3033.51750.17510.13550.02780.3220.0217-0.64250.14280.3811-0.14990.4116-0.085-0.00770.34850.0220.3908-28.4007-3.04252.9333
115.3347-1.72672.2743.14941.87584.48430.33890.86740.4608-0.19980.0058-0.9651-0.77650.98970.21470.3034-0.089-0.04190.47130.04940.4701-20.40593.399152.754
124.46271.02430.344.2540.08142.08480.1171-0.3120.69490.136-0.2748-0.077-0.29660.37940.22140.4465-0.1417-0.08310.4124-0.05690.4516-29.31067.920358.3834
134.5115-0.27952.5184.64170.30653.7692-0.29620.37990.2403-0.65780.0878-0.30710.02390.37570.21490.3964-0.06630.04390.37950.02470.3385-30.81413.14347.5341
141.88050.99771.61412.95460.39223.47110.19350.0055-0.20080.208-0.03470.07090.14460.2243-0.06950.2655-0.0517-0.0080.3318-0.01010.3591-38.3430.494949.5236
153.30011.91580.44755.82310.41293.3269-0.10790.2343-0.1241-0.61310.13280.3966-0.0266-0.4060.03040.4486-0.0848-0.13460.5133-0.00150.581-58.3929-15.071823.9532
164.4913.31970.80715.5696-0.04962.47490.0733-0.40350.72670.302-0.15991.0369-0.1746-0.53530.1670.41140.01230.02410.4833-0.01990.5439-58.0614-5.068634.5676
175.15753.09080.69685.87260.83491.6992-0.046-0.16080.5019-0.4299-0.15541.0701-0.192-0.30380.17790.4197-0.0135-0.10080.4563-0.01140.5196-58.7122-5.158429.9324
184.1773-0.27940.91112.36430.14285.10690.1068-0.0201-0.31190.21520.0468-0.40680.62060.4763-0.16760.490.0383-0.15510.35590.0010.4685-27.9097-21.441853.644
195.6759-0.78982.62374.3765-1.01935.82930.2595-0.5565-0.34090.31430.02260.12780.6958-0.4047-0.19130.5368-0.0273-0.02270.34170.05750.3414-31.551-19.534162.4509
207.48381.27433.92121.6242-0.26224.46630.8914-1.2917-0.11310.04-0.3821-0.00720.7365-0.5475-0.30990.7058-0.0568-0.11550.46250.05090.4022-37.4087-24.420256.4065
214.048-0.0611-1.22644.1111-0.70362.66480.0467-0.14360.00850.0411-0.0147-0.71410.04320.39590.05580.38740.0222-0.04440.40320.02460.4333-25.7935-13.029655.1642
221.89850.6159-0.29193.6168-0.65284.60330.01340.27770.0509-0.24590.06990.0251-0.13970.0543-0.16140.3844-0.0894-0.04070.425-0.01880.3507-46.142-16.301226.1467
231.3010.3441-0.15982.37540.76494.6131-0.0039-0.05550.10540.3559-0.05730.25560.3266-0.27660.05540.34070.0720.04080.4865-0.02280.4693-97.6196-15.26393.9237
243.8604-0.1919-1.44953.40771.46844.18120.23810.07360.2194-0.1738-0.06250.3438-0.4516-0.2795-0.19950.34290.10450.02290.42230.0060.4495-96.8669-5.864991.7499
253.4205-0.137-0.39932.57531.16231.2313-0.05210.1608-0.1491-0.42680.0765-0.41790.05080.1622-0.0950.35450.05340.01760.31420.04460.2845-74.2537-22.996670.9652
265.2158-1.20260.61332.38621.35891.29040.057-0.1866-0.1985-0.199-0.04180.12170.0952-0.03860.02390.43830.01570.0220.41440.07910.356-78.1955-25.384876.3581
276.7089-0.0799-0.0643.0913-0.15651.535-0.17070.1428-0.6488-0.4601-0.0146-0.36190.23190.25470.17220.49940.06060.07930.3571-0.02280.4514-68.0167-31.930571.5473
282.8293-1.6948-3.12783.05761.57785.03270.3819-0.48671.5285-0.39650.1494-0.4124-0.73470.7078-0.56150.5138-0.18750.16450.4071-0.13150.7471-73.43814.423590.9788
291.9665-0.75930.04333.92860.17574.62050.3197-0.54311.34520.04560.0984-0.1354-1.03470.0513-0.35960.60040.03390.18750.5005-0.24330.995-82.07667.832196.0579
303.0089-0.78080.12865.61841.19173.29850.2263-1.12681.00310.13380.266-0.6712-0.38350.5718-0.26850.6191-0.15580.19710.7988-0.380.8288-78.70734.1177102.2499
314.28511.0049-1.00974.18770.91924.59910.1941-0.56870.67060.19120.3567-0.2262-0.46820.6464-0.550.4919-0.11920.09620.6322-0.20590.5668-75.9275-1.693795.823
320.22920.40760.86223.0583-0.36214.80910.7564-0.34041.7246-0.0215-0.09640.1508-1.0649-0.3682-0.46010.67340.0070.19740.3128-0.08810.7034-88.38884.551294.3495
333.50740.31060.35992.22760.05494.9370.00460.15040.0999-0.21510.06010.11480.06290.0349-0.10290.3330.00920.05510.3043-0.00190.3893-68.8503-12.836671.3504
344.7931.07780.48793.19310.58276.46330.12920.4940.2209-0.41850.1291-0.19120.18580.1331-0.08950.41860.0240.07530.38110.04870.4301-68.8061-11.150363.6203
352.38430.9628-4.00554.83040.2797.5345-0.3531-0.0868-0.1526-0.55930.3002-0.2413-0.09940.8985-0.02740.4363-0.1376-0.00060.5432-0.0490.378-4.574128.116521.2513
366.38371.7634-0.51834.79650.78485.5873-0.52070.61310.295-0.74590.62690.4476-0.2676-0.1948-0.0160.5129-0.156-0.0650.42410.0720.3583-6.156535.559222.1065
372.77933.11341.03286.2956-2.78846.26430.28790.22740.2770.8419-0.0209-0.34790.2006-0.6705-0.37150.5627-0.06710.00040.4295-0.0270.4349-3.738931.227129.3506
387.64150.53191.50184.5731.27166.55470.0874-0.89410.01790.54670.16550.1767-0.02950.4071-0.33180.45910.00020.13490.56210.04750.4118-51.5839-4.175869.5718
393.8193-1.7469-1.36414.7527-0.53344.77670.02640.33390.08220.10660.2409-0.1368-0.1442-0.1637-0.12020.4001-0.03810.08090.44860.02150.4597-48.6922-0.353262.5897
405.08590.1765-1.93817.3455-1.48443.510.1082-0.10770.2361-0.5676-0.0135-1.06920.93030.4563-0.33360.5215-0.0971-0.1170.4548-0.10810.3516-44.0343-34.180823.4903
411.24720.553-0.55659.2141-5.83053.9296-0.2981-0.2095-0.31710.04880.1378-0.71661.4236-0.09650.10830.8346-0.0855-0.12160.5662-0.03490.458-43.498-35.969229.1022
423.73920.5106-0.31362.2731-2.80295.09210.15470.0137-0.01641.2360.13320.9404-0.11090.0743-0.28250.6804-0.05160.0360.5063-0.02330.4403-52.5143-35.700525.4427
434.29680.37271.62492.71870.85315.44790.25790.3922-0.07290.0499-0.2762-0.39740.58250.55930.26450.4297-0.01650.01590.3478-0.02780.4314-42.9619-43.088421.495
440.44850.9657-0.11925.48431.79681.27-0.40170.54790.4-1.20780.31820.22050.2028-0.1066-0.01860.4989-0.0159-0.11070.5991-0.0880.3247-47.3606-35.273215.0542
Refinement TLS group
IDRefine-IDRefine TLS-IDSelection details
1X-RAY DIFFRACTION1chain 'L' and (resid 2 through 48 )
2X-RAY DIFFRACTION2chain 'L' and (resid 49 through 128 )
3X-RAY DIFFRACTION3chain 'L' and (resid 129 through 163 )
4X-RAY DIFFRACTION4chain 'L' and (resid 164 through 213 )
5X-RAY DIFFRACTION5chain 'H' and (resid 1 through 95 )
6X-RAY DIFFRACTION6chain 'H' and (resid 96 through 111 )
7X-RAY DIFFRACTION7chain 'H' and (resid 112 through 136 )
8X-RAY DIFFRACTION8chain 'H' and (resid 137 through 229 )
9X-RAY DIFFRACTION9chain 'A' and (resid 2 through 29 )
10X-RAY DIFFRACTION10chain 'A' and (resid 30 through 48 )
11X-RAY DIFFRACTION11chain 'A' and (resid 49 through 61 )
12X-RAY DIFFRACTION12chain 'A' and (resid 62 through 75 )
13X-RAY DIFFRACTION13chain 'A' and (resid 76 through 90 )
14X-RAY DIFFRACTION14chain 'A' and (resid 91 through 113 )
15X-RAY DIFFRACTION15chain 'A' and (resid 114 through 128 )
16X-RAY DIFFRACTION16chain 'A' and (resid 129 through 163 )
17X-RAY DIFFRACTION17chain 'A' and (resid 164 through 213 )
18X-RAY DIFFRACTION18chain 'B' and (resid 1 through 39 )
19X-RAY DIFFRACTION19chain 'B' and (resid 40 through 75 )
20X-RAY DIFFRACTION20chain 'B' and (resid 76 through 87 )
21X-RAY DIFFRACTION21chain 'B' and (resid 88 through 111 )
22X-RAY DIFFRACTION22chain 'B' and (resid 112 through 229 )
23X-RAY DIFFRACTION23chain 'C' and (resid 2 through 29 )
24X-RAY DIFFRACTION24chain 'C' and (resid 30 through 102 )
25X-RAY DIFFRACTION25chain 'C' and (resid 103 through 137 )
26X-RAY DIFFRACTION26chain 'C' and (resid 138 through 174 )
27X-RAY DIFFRACTION27chain 'C' and (resid 175 through 213 )
28X-RAY DIFFRACTION28chain 'D' and (resid 1 through 17 )
29X-RAY DIFFRACTION29chain 'D' and (resid 18 through 40 )
30X-RAY DIFFRACTION30chain 'D' and (resid 41 through 82 )
31X-RAY DIFFRACTION31chain 'D' and (resid 82A through 95 )
32X-RAY DIFFRACTION32chain 'D' and (resid 96 through 111 )
33X-RAY DIFFRACTION33chain 'D' and (resid 112 through 184 )
34X-RAY DIFFRACTION34chain 'D' and (resid 185 through 228 )
35X-RAY DIFFRACTION35chain 'M' and (resid 5 through 27 )
36X-RAY DIFFRACTION36chain 'M' and (resid 28 through 51 )
37X-RAY DIFFRACTION37chain 'M' and (resid 52 through 64 )
38X-RAY DIFFRACTION38chain 'N' and (resid 5 through 46 )
39X-RAY DIFFRACTION39chain 'N' and (resid 47 through 64 )
40X-RAY DIFFRACTION40chain 'O' and (resid 5 through 17 )
41X-RAY DIFFRACTION41chain 'O' and (resid 18 through 27 )
42X-RAY DIFFRACTION42chain 'O' and (resid 28 through 46 )
43X-RAY DIFFRACTION43chain 'O' and (resid 47 through 55 )
44X-RAY DIFFRACTION44chain 'O' and (resid 56 through 63 )

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