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Open data
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Basic information
Entry | Database: PDB / ID: 6nzn | ||||||
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Title | Dimer-of-dimer amyloid fibril structure of glucagon | ||||||
![]() | Glucagon | ||||||
![]() | HORMONE / PROTEIN FIBRIL / amyloid | ||||||
Function / homology | ![]() glucagon receptor binding / negative regulation of execution phase of apoptosis / feeding behavior / positive regulation of calcium ion import / cellular response to glucagon stimulus / positive regulation of insulin secretion involved in cellular response to glucose stimulus / regulation of insulin secretion / Synthesis, secretion, and deacylation of Ghrelin / positive regulation of gluconeogenesis / protein kinase A signaling ...glucagon receptor binding / negative regulation of execution phase of apoptosis / feeding behavior / positive regulation of calcium ion import / cellular response to glucagon stimulus / positive regulation of insulin secretion involved in cellular response to glucose stimulus / regulation of insulin secretion / Synthesis, secretion, and deacylation of Ghrelin / positive regulation of gluconeogenesis / protein kinase A signaling / positive regulation of peptidyl-threonine phosphorylation / response to activity / gluconeogenesis / adenylate cyclase-modulating G protein-coupled receptor signaling pathway / adenylate cyclase-activating G protein-coupled receptor signaling pathway / hormone activity / Glucagon signaling in metabolic regulation / Synthesis, secretion, and inactivation of Glucagon-like Peptide-1 (GLP-1) / Glucagon-type ligand receptors / Glucagon-like Peptide-1 (GLP1) regulates insulin secretion / positive regulation of peptidyl-serine phosphorylation / glucose homeostasis / G alpha (s) signalling events / G alpha (q) signalling events / secretory granule lumen / positive regulation of ERK1 and ERK2 cascade / G protein-coupled receptor signaling pathway / endoplasmic reticulum lumen / signaling receptor binding / negative regulation of apoptotic process / extracellular space / extracellular region / identical protein binding / plasma membrane Similarity search - Function | ||||||
Biological species | ![]() | ||||||
Method | SOLID-STATE NMR / torsion angle dynamics | ||||||
![]() | Gelenter, M.D. / Smith, K.J. / Liao, S.Y. / Mandala, V.S. / Dregni, A.J. / Lamm, M.S. / Tian, Y. / Wei, X. / Pochan, D.J. / Tucker, T.J. ...Gelenter, M.D. / Smith, K.J. / Liao, S.Y. / Mandala, V.S. / Dregni, A.J. / Lamm, M.S. / Tian, Y. / Wei, X. / Pochan, D.J. / Tucker, T.J. / Su, Y. / Hong, M. | ||||||
Funding support | ![]()
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![]() | ![]() Title: The peptide hormone glucagon forms amyloid fibrils with two coexisting beta-strand conformations. Authors: Gelenter, M.D. / Smith, K.J. / Liao, S.Y. / Mandala, V.S. / Dregni, A.J. / Lamm, M.S. / Tian, Y. / Xu, W. / Pochan, D.J. / Tucker, T.J. / Su, Y. / Hong, M. | ||||||
History |
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Remark 700 | SHEET DETERMINATION METHOD: AUTHOR |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 1.7 MB | Display | ![]() |
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PDB format | ![]() | 1.4 MB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
Similar structure data | |
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Other databases |
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Links
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Assembly
Deposited unit | ![]()
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NMR ensembles |
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Components
#1: Protein/peptide | Mass: 3486.781 Da / Num. of mol.: 16 / Source method: obtained synthetically / Source: (synth.) ![]() |
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-Experimental details
-Experiment
Experiment | Method: SOLID-STATE NMR | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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NMR experiment |
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Sample preparation
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Sample |
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Sample conditions | Ionic strength: 0.01 M / Ionic strength err: 0.002 / Label: 1 / pH: 2 / PH err: 0.1 / Pressure: 1 atm / Pressure err: 0.01 / Temperature: 293 K / Temperature err: 0.2 |
-NMR measurement
NMR spectrometer |
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Processing
NMR software |
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Refinement | Method: torsion angle dynamics / Software ordinal: 1 | ||||||||||||||||||||||||
NMR representative | Selection criteria: target function | ||||||||||||||||||||||||
NMR ensemble | Conformer selection criteria: target function / Conformers calculated total number: 700 / Conformers submitted total number: 10 |