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Yorodumi- SASDBU6: Apo form of the C-terminal deletion mutant of ObgE from E.coli (O... -
+Open data
-Basic information
Entry | Database: SASBDB / ID: SASDBU6 |
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Sample | Apo form of the C-terminal deletion mutant of ObgE from E.coli (ObgE_340)
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Function / homology | Function and homology information guanyl ribonucleotide binding / dormancy process / negative regulation of ribosome biogenesis / guanosine tetraphosphate binding / ribosomal large subunit binding / ribosome assembly / chromosome segregation / Hydrolases; Acting on acid anhydrides; Acting on GTP to facilitate cellular and subcellular movement / GDP binding / rRNA binding ...guanyl ribonucleotide binding / dormancy process / negative regulation of ribosome biogenesis / guanosine tetraphosphate binding / ribosomal large subunit binding / ribosome assembly / chromosome segregation / Hydrolases; Acting on acid anhydrides; Acting on GTP to facilitate cellular and subcellular movement / GDP binding / rRNA binding / GTPase activity / GTP binding / magnesium ion binding / DNA binding / cytosol Similarity search - Function |
Biological species | Escherichia coli (strain K12) (bacteria) |
Contact author |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Models
Model #738 | Type: atomic / Software: RANCH/FULCHER/GAJOE / Radius of dummy atoms: 1.90 A / Symmetry: P1 / Chi-square value: 6.564 Search similar-shape structures of this assembly by Omokage search (details) |
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Model #739 | Type: atomic / Software: RANCH/FULCHER/GAJOE / Radius of dummy atoms: 1.90 A / Symmetry: P1 / Chi-square value: 6.564 Search similar-shape structures of this assembly by Omokage search (details) |
Model #740 | Type: atomic / Software: RANCH/FULCHER/GAJOE / Radius of dummy atoms: 1.90 A / Symmetry: P1 / Chi-square value: 6.564 Search similar-shape structures of this assembly by Omokage search (details) |
Model #742 | Type: atomic / Software: RANCH/FULCHER/GAJOE / Radius of dummy atoms: 1.90 A / Symmetry: P1 / Chi-square value: 6.564 Search similar-shape structures of this assembly by Omokage search (details) |
Model #737 | Type: dummy / Software: DAMMIN (r7897M) / Radius of dummy atoms: 2.50 A / Symmetry: P1 / Chi-square value: 7.778 Search similar-shape structures of this assembly by Omokage search (details) |
-Sample
Sample | Name: Apo form of the C-terminal deletion mutant of ObgE from E.coli (ObgE_340) Specimen concentration: 7 mg/ml |
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Buffer | Name: 20 mM Hepes , 300 mM NaCl, 250 mM imidazole, 5 mM MgCl2, 2 mM DTT pH: 7.5 |
Entity #421 | Name: ObgE_340 / Type: protein / Description: GTPase ObgE/CgtA / Formula weight: 38.899 / Num. of mol.: 1 / Source: Escherichia coli (strain K12) / References: UniProt: P42641 Sequence: MGSSHHHHHH SSGLVPRGSH MKFVDEASIL VVAGDGGNGC VSFRREKYIP KGGPDGGDGG DGGDVWMEAD ENLNTLIDYR FEKSFRAERG QNGASRDCTG KRGKDVTIKV PVGTRVIDQG TGETMGDMTK HGQRLLVAKG GWHGLGNTRF KSSVNRTPRQ KTNGTPGDKR ...Sequence: MGSSHHHHHH SSGLVPRGSH MKFVDEASIL VVAGDGGNGC VSFRREKYIP KGGPDGGDGG DGGDVWMEAD ENLNTLIDYR FEKSFRAERG QNGASRDCTG KRGKDVTIKV PVGTRVIDQG TGETMGDMTK HGQRLLVAKG GWHGLGNTRF KSSVNRTPRQ KTNGTPGDKR ELLLELMLLA DVGMLGMPNA GKSTFIRAVS AAKPKVADYP FTTLVPSLGV VRMDNEKSFV VADIPGLIEG AAEGAGLGIR FLKHLERCRV LLHLIDIDPI DGTDPVENAR IIISELELYS QDLATKPRWL VFNKIDLLDK VEAEEKAKAI AEALGWEDKY YLISAASGLG VKDLCWDVMT FIIENPVA |
-Experimental information
Beam | Instrument name: SOLEIL SWING / City: Saint-Aubin / 国: France / Type of source: X-ray synchrotron / Wavelength: 0.103 Å / Dist. spec. to detc.: 1.79 mm | |||||||||||||||||||||||||||||||||
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Detector | Name: AVIEX / Type: CCD | |||||||||||||||||||||||||||||||||
Scan | Title: Apo form of the C-terminal deletion mutant of ObgE / Measurement date: Mar 12, 2015 / Storage temperature: 10 °C / Cell temperature: 10 °C / Exposure time: 2.8 sec. / Number of frames: 255 / Unit: 1/A /
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Distance distribution function P(R) | Sofotware P(R): GNOM 4.6 / Number of points: 271 /
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Result | Type of curve: other Comments: The sample protein includes an N-terminal poly-histidine tag; frames corresponding to the peak in HPLC-SAXS run were averaged using DATAVER v0.2 (r3709)
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