温度: 293 K / 手法: 蒸気拡散法, ハンギングドロップ法 詳細: Protein, at the concentration 15 mg/ml in 50 mM HEPES buffer pH 7 and 150 mM sodium chloride was mixed with equivolume solution of precipitant that contained, 17% (w/v) PEG3350, 0.17 M ...詳細: Protein, at the concentration 15 mg/ml in 50 mM HEPES buffer pH 7 and 150 mM sodium chloride was mixed with equivolume solution of precipitant that contained, 17% (w/v) PEG3350, 0.17 M ammonium citrate pH 5.0, and 20 mM L-Asn. Resulting droplets were equilibrated against the precipitant. For the data collection, crystal was mounted in a quartz capillary PH範囲: 5.3-5.4 / Temp details: incubator-controlled
-
データ収集
回折
平均測定温度: 298 K / Ambient temp details: room temperature / Serial crystal experiment: N
構造決定の手法: フーリエ合成 / 解像度: 2.15→40 Å / Cor.coef. Fo:Fc: 0.978 / Cor.coef. Fo:Fc free: 0.96 / SU B: 3.858 / SU ML: 0.095 / SU R Cruickshank DPI: 0.1455 / 交差検証法: THROUGHOUT / σ(F): 0 / ESU R: 0.146 / ESU R Free: 0.135 詳細: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS U VALUES : REFINED INDIVIDUALLY