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Open data
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Basic information
Entry | Database: PDB / ID: 6nxc | ||||||
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Title | ECAI(T162A) MUTANT IN COMPLEX WITH CITRATE AT PH 4 | ||||||
![]() | L-asparaginase 1 | ||||||
![]() | HYDROLASE / hydrolysis of L-asparagine | ||||||
Function / homology | ![]() asparagine catabolic process via L-aspartate / asparaginase / asparaginase activity / protein homotetramerization / identical protein binding / cytoplasm / cytosol Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() | ||||||
![]() | Lubkowski, J. / Wlodawer, A. | ||||||
![]() | ![]() Title: Crystal structure and allosteric regulation of the cytoplasmic Escherichia coli L-asparaginase I. Authors: Yun, M.K. / Nourse, A. / White, S.W. / Rock, C.O. / Heath, R.J. | ||||||
History |
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Remark 0 | THIS ENTRY 6NXC REFLECTS AN ALTERNATIVE MODELING OF THE STRUCTURAL DATA IN R2HIMSF ORIGINAL DATA ...THIS ENTRY 6NXC REFLECTS AN ALTERNATIVE MODELING OF THE STRUCTURAL DATA IN R2HIMSF ORIGINAL DATA DETERMINED BY AUTHOR: M.K.YUN,A.NOURSE,S.W.WHITE,C.O.ROCK,R.J.HEATH. |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 275.7 KB | Display | ![]() |
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PDB format | ![]() | 227.4 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 2.9 MB | Display | ![]() |
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Full document | ![]() | 2.9 MB | Display | |
Data in XML | ![]() | 56.1 KB | Display | |
Data in CIF | ![]() | 78 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 6nx6C ![]() 6nx7C ![]() 6nx8C ![]() 6nx9C ![]() 6nxaC ![]() 6nxbC ![]() 6nxdC C: citing same article ( |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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1 |
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Unit cell |
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Components
-Protein , 1 types, 4 molecules ABCD
#1: Protein | Mass: 39190.348 Da / Num. of mol.: 4 / Mutation: T162A Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() Strain: K12 / Gene: ansA, b1767, JW1756 / Plasmid: pET-15b / Production host: ![]() ![]() |
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-Non-polymers , 6 types, 595 molecules ![](data/chem/img/CIT.gif)
![](data/chem/img/EDO.gif)
![](data/chem/img/CL.gif)
![](data/chem/img/GOL.gif)
![](data/chem/img/ASN.gif)
![](data/chem/img/HOH.gif)
![](data/chem/img/EDO.gif)
![](data/chem/img/CL.gif)
![](data/chem/img/GOL.gif)
![](data/chem/img/ASN.gif)
![](data/chem/img/HOH.gif)
#2: Chemical | ChemComp-CIT / #3: Chemical | ChemComp-EDO / #4: Chemical | ChemComp-CL / #5: Chemical | ChemComp-GOL / | #6: Chemical | ChemComp-ASN / #7: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.15 Å3/Da / Density % sol: 42.67 % / Description: AUTHOR USED THE SF DATA FROM ENTRY 2HIM |
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Crystal grow | Temperature: 291 K / Method: vapor diffusion, hanging drop / pH: 4 Details: Citrate buffer, Sodium Chloride, pH 4.0, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: MARRESEARCH / Detector: CCD / Date: Feb 28, 2006 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.97923 Å / Relative weight: 1 |
Reflection | Resolution: 1.74→50 Å / Num. obs: 132353 / % possible obs: 98.3 % / Observed criterion σ(I): -3 / Redundancy: 3 % / Net I/σ(I): 14.8 |
Reflection shell | Resolution: 1.74→1.81 Å |
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Processing
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Refinement | Resolution: 1.74→46.54 Å / Cor.coef. Fo:Fc: 0.961 / Cor.coef. Fo:Fc free: 0.946 / SU B: 2.236 / SU ML: 0.072 / SU R Cruickshank DPI: 0.113 / Cross valid method: THROUGHOUT / σ(F): 0 / ESU R: 0.113 / ESU R Free: 0.113 Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS U VALUES : REFINED INDIVIDUALLY
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso max: 133.73 Å2 / Biso mean: 25.698 Å2 / Biso min: 11.57 Å2
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Refinement step | Cycle: final / Resolution: 1.74→46.54 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 1.744→1.79 Å / Rfactor Rfree error: 0 / Total num. of bins used: 20
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