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- PDB-6n6f: Vibrio cholerae Oligoribonuclease bound to pGC -

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Basic information

Entry
Database: PDB / ID: 6n6f
TitleVibrio cholerae Oligoribonuclease bound to pGC
Components
  • OligoribonucleaseOligonucleotidase
  • RNA (5'-R(P*GP*C)-3')
Keywordsrna binding protein/rna / 3'-5' exoribonuclease / RNA BINDING PROTEIN / rna binding protein-rna complex
Function / homology
Function and homology information


Hydrolases; Acting on ester bonds; Exonucleases that are active with either ribo- or deoxyribonucleic acids and produce 5'-phosphomonoesters / 3'-5'-RNA exonuclease activity / Hydrolases; Acting on ester bonds / nucleic acid binding / cytoplasm
Similarity search - Function
Oligoribonuclease / Exonuclease / Exonuclease, RNase T/DNA polymerase III / EXOIII / Ribonuclease H-like superfamily/Ribonuclease H / Nucleotidyltransferase; domain 5 / Ribonuclease H superfamily / Ribonuclease H-like superfamily / 2-Layer Sandwich / Alpha Beta
Similarity search - Domain/homology
RNA / Oligoribonuclease / Oligoribonuclease
Similarity search - Component
Biological speciesVibrio cholerae (bacteria)
synthetic construct (others)
MethodX-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 1.735 Å
AuthorsLormand, J.D. / Sondermann, H.
Funding support United States, 1items
OrganizationGrant numberCountry
National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)R01GM123609 United States
CitationJournal: Elife / Year: 2019
Title: A dedicated diribonucleotidase resolves a key bottleneck for the terminal step of RNA degradation.
Authors: Kim, S.K. / Lormand, J.D. / Weiss, C.A. / Eger, K.A. / Turdiev, H. / Turdiev, A. / Winkler, W.C. / Sondermann, H. / Lee, V.T.
History
DepositionNov 26, 2018Deposition site: RCSB / Processing site: RCSB
Revision 1.0Jun 12, 2019Provider: repository / Type: Initial release
Revision 1.1Oct 16, 2019Group: Data collection / Database references / Category: citation / citation_author
Item: _citation.country / _citation.journal_abbrev ..._citation.country / _citation.journal_abbrev / _citation.journal_id_CSD / _citation.journal_id_ISSN / _citation.journal_volume / _citation.pdbx_database_id_DOI / _citation.pdbx_database_id_PubMed / _citation.title / _citation.year
Revision 1.2Jan 1, 2020Group: Author supporting evidence / Category: pdbx_audit_support / Item: _pdbx_audit_support.funding_organization

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
A: Oligoribonuclease
D: RNA (5'-R(P*GP*C)-3')
hetero molecules


Theoretical massNumber of molelcules
Total (without water)21,5833
Polymers21,5602
Non-polymers231
Water4,017223
1


  • Idetical with deposited unit
  • defined by author&software
  • Evidence: light scattering
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area1040 Å2
ΔGint-24 kcal/mol
Surface area9110 Å2
MethodPISA
Unit cell
Length a, b, c (Å)88.715, 88.715, 59.780
Angle α, β, γ (deg.)90.000, 90.000, 120.000
Int Tables number154
Space group name H-MP3221
Components on special symmetry positions
IDModelComponents
11A-405-

HOH

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Components

#1: Protein Oligoribonuclease / Oligonucleotidase


Mass: 20954.910 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Vibrio cholerae (bacteria) / Gene: orn / Plasmid: pET28a / Production host: Escherichia coli BL21(DE3) (bacteria) / Strain (production host): BL21DE3
References: UniProt: A0A0H6V4D7, UniProt: Q9KV17*PLUS, Hydrolases; Acting on ester bonds
#2: RNA chain RNA (5'-R(P*GP*C)-3')


Mass: 605.430 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others)
#3: Chemical ChemComp-NA / SODIUM ION


Mass: 22.990 Da / Num. of mol.: 1 / Source method: obtained synthetically / Formula: Na
#4: Water ChemComp-HOH / water / Water


Mass: 18.015 Da / Num. of mol.: 223 / Source method: isolated from a natural source / Formula: H2O

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Experimental details

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Experiment

ExperimentMethod: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

CrystalDensity Matthews: 3.15 Å3/Da / Density % sol: 60.95 %
Crystal growTemperature: 293 K / Method: vapor diffusion, hanging drop / pH: 5.5
Details: 0.1 M BisTris (pH 5.5), 17% polyethylene glycol 3350, and 20% xylitol

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Data collection

DiffractionMean temperature: 100 K / Serial crystal experiment: N
Diffraction sourceSource: SYNCHROTRON / Site: CHESS / Beamline: F1 / Wavelength: 0.977 Å
DetectorType: DECTRIS PILATUS 6M / Detector: PIXEL / Date: Mar 14, 2018
RadiationMonochromator: Si(111) crystal / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelengthWavelength: 0.977 Å / Relative weight: 1
ReflectionResolution: 1.735→47.18 Å / Num. all: 28405 / Num. obs: 28405 / % possible obs: 99.8 % / Redundancy: 11.9 % / Rpim(I) all: 0.016 / Rrim(I) all: 0.056 / Rsym value: 0.054 / Net I/av σ(I): 9.6 / Net I/σ(I): 24 / Num. measured all: 338345
Reflection shell

Diffraction-ID: 1

Resolution (Å)Redundancy (%)Rmerge(I) obsMean I/σ(I) obsNum. unique obsRpim(I) allRrim(I) allRsym value% possible all
1.74-1.8311.61.0330.840500.3121.081.03398.9
1.83-1.9411.80.581.339090.1750.6060.58100
1.94-2.0712.20.3122.536430.0930.3260.312100
2.07-2.2412.30.1724.534030.0510.1790.172100
2.24-2.45120.109731550.0330.1140.109100
2.45-2.7412.10.07110.328720.0210.0750.071100
2.74-3.1711.90.04714.125400.0140.0490.047100
3.17-3.8811.80.03517.921480.010.0360.035100
3.88-5.4911.50.02920.917040.0090.030.029100
5.49-47.1811.10.027209810.0080.0280.02799.9

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Processing

Software
NameVersionClassification
XDSdata reduction
SCALA3.3.22data scaling
PHENIX1.14_3260refinement
PDB_EXTRACT3.24data extraction
PHENIXphasing
RefinementMethod to determine structure: MOLECULAR REPLACEMENT
Starting model: 6N6A

6n6a


Resolution: 1.735→32.317 Å / SU ML: 0.17 / Cross valid method: THROUGHOUT / σ(F): 1.35 / Phase error: 17.67
RfactorNum. reflection% reflection
Rfree0.1699 1421 5.01 %
Rwork0.1547 --
obs0.1555 28374 99.82 %
Solvent computationShrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å
Displacement parametersBiso max: 95.51 Å2 / Biso mean: 36.3173 Å2 / Biso min: 21.06 Å2
Refinement stepCycle: final / Resolution: 1.735→32.317 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms1464 44 1 226 1735
Biso mean--31.09 48.31 -
Num. residues----182
LS refinement shell

Refine-ID: X-RAY DIFFRACTION / Rfactor Rfree error: 0 / Total num. of bins used: 10

Resolution (Å)Rfactor RfreeNum. reflection RfreeRfactor RworkNum. reflection RworkNum. reflection all% reflection obs (%)
1.7352-1.79720.2591370.25762615275298
1.7972-1.86920.23641350.210526882823100
1.8692-1.95420.22541500.190326532803100
1.9542-2.05730.21121380.168827022840100
2.0573-2.18610.18381420.15426562798100
2.1861-2.35490.15761350.156926892824100
2.3549-2.59180.15621470.159626752822100
2.5918-2.96660.17851380.156627302868100
2.9666-3.73670.16881510.143727262877100
3.7367-32.3230.14841480.14328192967100
Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

IDL11 (°2)L12 (°2)L13 (°2)L22 (°2)L23 (°2)L33 (°2)S11 (Å °)S12 (Å °)S13 (Å °)S21 (Å °)S22 (Å °)S23 (Å °)S31 (Å °)S32 (Å °)S33 (Å °)T11 (Å2)T12 (Å2)T13 (Å2)T22 (Å2)T23 (Å2)T33 (Å2)Origin x (Å)Origin y (Å)Origin z (Å)
14.2953-1.2361-1.04073.62360.74682.4181-0.22250.003-0.2328-0.17120.108-0.13610.24020.03350.120.2717-0.02660.020.1483-0.00770.2069-44.3726.4781.428
23.1969-1.0243-1.81162.2110.64392.80820.00650.3421-0.0087-0.3351-0.05590.0531-0.081-0.25430.04430.2762-0.0212-0.00070.20410.0010.1775-47.08413.746-2.343
32.4515-0.84250.80581.6647-1.0411.5584-0.1003-0.0711-0.017-0.0002-0.058-0.3328-0.10860.12780.17270.2541-0.02670.04410.2166-0.01940.281-34.9026.1636.521
Refinement TLS group
IDRefine-IDRefine TLS-IDSelection detailsAuth asym-IDAuth seq-ID
1X-RAY DIFFRACTION1( CHAIN A AND RESID 2:24 )A2 - 24
2X-RAY DIFFRACTION2( CHAIN A AND RESID 25:99 )A25 - 99
3X-RAY DIFFRACTION3( CHAIN A AND RESID 100:181 )A100 - 181

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