- PDB-6n64: Crystal structure of mouse SMCHD1 hinge domain -
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Basic information
Entry
Database: PDB / ID: 6n64
Title
Crystal structure of mouse SMCHD1 hinge domain
Components
Structural maintenance of chromosomes flexible hinge domain-containing protein 1
Uncharacterized peptide from Structural maintenance of chromosomes flexible hinge domain-containing protein 1
Keywords
DNA BINDING PROTEIN / SMCHD1 Epigenetic control DNA binding SMC protein
Function / homology
Function and homology information
: / : / : / nose development / chromosome, telomeric region => GO:0000781 / Barr body / dosage compensation by inactivation of X chromosome / positive regulation of double-strand break repair via nonhomologous end joining / Hydrolases; Acting on acid anhydrides; In phosphorus-containing anhydrides / positive regulation of DNA repair ...: / : / : / nose development / chromosome, telomeric region => GO:0000781 / Barr body / dosage compensation by inactivation of X chromosome / positive regulation of double-strand break repair via nonhomologous end joining / Hydrolases; Acting on acid anhydrides; In phosphorus-containing anhydrides / positive regulation of DNA repair / negative regulation of double-strand break repair via homologous recombination / double-strand break repair / site of double-strand break / protein homodimerization activity / ATP hydrolysis activity / DNA binding / ATP binding Similarity search - Function
Structural maintenance of chromosomes flexible hinge domain-containing protein 1 / SMCs flexible hinge / SMCs flexible hinge superfamily / SMC proteins Flexible Hinge Domain / SMC proteins Flexible Hinge Domain / Histidine kinase/HSP90-like ATPase superfamily Similarity search - Domain/homology
A: Structural maintenance of chromosomes flexible hinge domain-containing protein 1 B: Structural maintenance of chromosomes flexible hinge domain-containing protein 1 C: Structural maintenance of chromosomes flexible hinge domain-containing protein 1 D: Structural maintenance of chromosomes flexible hinge domain-containing protein 1 E: Structural maintenance of chromosomes flexible hinge domain-containing protein 1 F: Structural maintenance of chromosomes flexible hinge domain-containing protein 1 G: Uncharacterized peptide from Structural maintenance of chromosomes flexible hinge domain-containing protein 1 H: Uncharacterized peptide from Structural maintenance of chromosomes flexible hinge domain-containing protein 1
A: Structural maintenance of chromosomes flexible hinge domain-containing protein 1 B: Structural maintenance of chromosomes flexible hinge domain-containing protein 1
C: Structural maintenance of chromosomes flexible hinge domain-containing protein 1 D: Structural maintenance of chromosomes flexible hinge domain-containing protein 1
E: Structural maintenance of chromosomes flexible hinge domain-containing protein 1 F: Structural maintenance of chromosomes flexible hinge domain-containing protein 1
Mass: 1805.216 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Mus musculus (house mouse) / Gene: Smchd1, Kiaa0650 / Production host: Escherichia coli (E. coli) References: Hydrolases; Acting on acid anhydrides; In phosphorus-containing anhydrides
Has ligand of interest
N
Sequence details
Chains G and H are portions of helix that are included in the SMCHD1 construct, but could not be ...Chains G and H are portions of helix that are included in the SMCHD1 construct, but could not be assigned to one of the chains A-F. In addition the resolution was not sufficient to dock sequence so they have been modeled as poly alanine helices, and since the registry is unknown, they are marked as UNKs. There should be 6 copies of these, but only 2 were sufficiently ordered to model as helices.
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Experimental details
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Experiment
Experiment
Method: X-RAY DIFFRACTION / Number of used crystals: 1
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Sample preparation
Crystal
Density Matthews: 2.85 Å3/Da / Density % sol: 56.83 %
Crystal grow
Temperature: 281 K / Method: vapor diffusion / pH: 9.35 Details: 0.1 M Sodium Bicine pH 9.35, 0.18 M MgCl2, 20% (v/v) 2-propanol
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Data collection
Diffraction
Mean temperature: 100 K / Serial crystal experiment: N
Diffraction source
Source: SYNCHROTRON / Site: Australian Synchrotron / Beamline: MX2 / Wavelength: 0.9537 Å
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