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Open data
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Basic information
Entry | Database: PDB / ID: 6mvm | ||||||||||||
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Title | LasR LBD L130F:3OC14HSL complex | ||||||||||||
![]() | Transcriptional regulator LasR | ||||||||||||
![]() | TRANSCRIPTION / transcriptional activator protein / 3-Oxo-N-(2-oxooxolan-3-yl)tetradecanamide | ||||||||||||
Function / homology | ![]() | ||||||||||||
Biological species | ![]() ![]() | ||||||||||||
Method | ![]() ![]() | ||||||||||||
![]() | Paczkowski, J.E. / Bassler, B.L. | ||||||||||||
Funding support | ![]()
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![]() | ![]() Title: An Autoinducer Analogue Reveals an Alternative Mode of Ligand Binding for the LasR Quorum-Sensing Receptor. Authors: Paczkowski, J.E. / McCready, A.R. / Cong, J.P. / Li, Z. / Jeffrey, P.D. / Smith, C.D. / Henke, B.R. / Hughson, F.M. / Bassler, B.L. #1: ![]() Title: Structural determinants driving homoserine lactone ligand selection in thePseudomonas aeruginosaLasR quorum-sensing receptor. Authors: McCready, A.R. / Paczkowski, J.E. / Henke, B.R. / Bassler, B.L. | ||||||||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 82.2 KB | Display | ![]() |
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PDB format | ![]() | 60.4 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 882.9 KB | Display | ![]() |
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Full document | ![]() | 885.6 KB | Display | |
Data in XML | ![]() | 15.7 KB | Display | |
Data in CIF | ![]() | 21.4 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 6mwhC ![]() 6mwlC ![]() 6mwwC ![]() 6mwzC ![]() 2uv0S S: Starting model for refinement C: citing same article ( |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 18220.516 Da / Num. of mol.: 2 / Fragment: UNP residues 7-168 / Mutation: L130F Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() Strain: UCBPP-PA14 / Gene: lasR, PA14_45960 / Production host: ![]() ![]() #2: Chemical | #3: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.35 Å3/Da / Density % sol: 47.56 % |
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Crystal grow | Temperature: 298 K / Method: vapor diffusion, hanging drop / Details: 200 mM magnesium nitrate, 20% PEG3350 |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N |
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Diffraction source | Source: ![]() |
Detector | Type: RIGAKU RAXIS IV++ / Detector: IMAGE PLATE / Date: Dec 7, 2017 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1 Å / Relative weight: 1 |
Reflection | Resolution: 1.895→30 Å / Num. obs: 25887 / % possible obs: 96.6 % / Redundancy: 2.5 % / Net I/σ(I): 14.4 |
Reflection shell | Resolution: 1.9→1.98 Å |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: PDB entry 2UV0 Resolution: 1.895→29.568 Å / Cross valid method: FREE R-VALUE / σ(F): 0 / Phase error: 31.58
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 1.895→29.568 Å
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Refine LS restraints |
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LS refinement shell |
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