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- PDB-2uv0: Structure of the P. aeruginosa LasR ligand-binding domain bound t... -
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Open data
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Basic information
Entry | Database: PDB / ID: 2uv0 | ||||||
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Title | Structure of the P. aeruginosa LasR ligand-binding domain bound to its autoinducer | ||||||
![]() | TRANSCRIPTIONAL ACTIVATOR PROTEIN LASR | ||||||
![]() | TRANSCRIPTION / ACYL-HOMOSERINE LACTONE RECEPTOR / QUORUM SENSING / TRANSCRIPTION REGULATION / ALPHA-BETA-ALPHA SANDWICH | ||||||
Function / homology | ![]() positive regulation of elastin biosynthetic process / regulation of elastin catabolic process / quorum sensing / DNA-binding transcription activator activity / protein-DNA complex / regulation of gene expression / sequence-specific DNA binding / transcription cis-regulatory region binding / DNA-binding transcription factor activity / regulation of DNA-templated transcription / positive regulation of DNA-templated transcription Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Bottomley, M.J. / Muraglia, E. / Bazzo, R. / Carfi, A. | ||||||
![]() | ![]() Title: Molecular Insights Into Quorum Sensing in the Human Pathogen Pseudomonas Aeruginosa from the Structure of the Virulence Regulator Lasr Bound to its Autoinducer. Authors: Bottomley, M.J. / Muraglia, E. / Bazzo, R. / Carfi, A. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 143.2 KB | Display | ![]() |
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PDB format | ![]() | 120.8 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 1.2 MB | Display | ![]() |
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Full document | ![]() | 1.2 MB | Display | |
Data in XML | ![]() | 31.4 KB | Display | |
Data in CIF | ![]() | 45.5 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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2 | ![]()
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Unit cell |
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Noncrystallographic symmetry (NCS) | NCS oper:
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Components
#1: Protein | Mass: 19686.465 Da / Num. of mol.: 4 / Fragment: LIGAND-BINDING DOMAIN, RESIDUES 1-173 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() Description: PSEUDOMONAS GENETIC STOCK CENTER, GREENVILLE, NC, USA Plasmid: PETM11 / Production host: ![]() ![]() #2: Chemical | ChemComp-OHN / #3: Water | ChemComp-HOH / | Sequence details | THE N-TERMINAL GLY-ALA IS A REMNANT OF THE AFFINITY- PURIFICATI | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.22 Å3/Da / Density % sol: 44.6 % |
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Crystal grow | pH: 7.3 Details: 20% W/V PEG 4000, 80 MM CALCIUM ACETATE, 40 MM HEPES PH 7.3, 5 MM DTT AND 50 MICROMOLAR N-3-OXO-DODECANOYL-HOMOSERINE LACTONE. |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: MARRESEARCH / Detector: CCD / Date: Feb 21, 2005 / Details: MIRRORS |
Radiation | Monochromator: SI(111) / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.98 Å / Relative weight: 1 |
Reflection | Resolution: 1.8→40 Å / Num. obs: 61495 / % possible obs: 97.8 % / Observed criterion σ(I): 2 / Redundancy: 8.1 % / Rmerge(I) obs: 0.09 / Net I/σ(I): 26.4 |
Reflection shell | Resolution: 1.8→1.9 Å / Redundancy: 7.5 % / Rmerge(I) obs: 0.58 / Mean I/σ(I) obs: 3.1 / % possible all: 96.9 |
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Processing
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Refinement | Method to determine structure: ![]() Stereochemistry target values: MAXIMUM LIKELIHOODWITH PHASES Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS. OF THE EXPECTED 175 RESIDUE PER CHAIN, ALL RESIDUES WERE VISIBLE EXCEPT FOR A FEW (USUALLY LESS THAN 5) AT THE N- AND C-TERMINI.
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.4 Å / Solvent model: MASK | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 3.69 Å2
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Refinement step | Cycle: LAST / Resolution: 1.8→40 Å
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