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- PDB-6l7s: Crystal structure of FKRP in complex with Mg ion, Zinc peak data -

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Basic information

Entry
Database: PDB / ID: 6l7s
TitleCrystal structure of FKRP in complex with Mg ion, Zinc peak data
ComponentsFukutin-related protein
KeywordsTRANSFERASE / glycosyltranferase / phospho ribitoyl tranferase
Function / homology
Function and homology information


pentitol metabolic process / filtration diaphragm assembly / pentose metabolic process / connective tissue development / creatine metabolic process / connective tissue replacement / Transferases; Transferring phosphorus-containing groups; Transferases for other substituted phosphate groups / phosphotransferase activity, for other substituted phosphate groups / oxygen metabolic process / localization of cell ...pentitol metabolic process / filtration diaphragm assembly / pentose metabolic process / connective tissue development / creatine metabolic process / connective tissue replacement / Transferases; Transferring phosphorus-containing groups; Transferases for other substituted phosphate groups / phosphotransferase activity, for other substituted phosphate groups / oxygen metabolic process / localization of cell / diaphragm development / maintenance of protein localization in endoplasmic reticulum / protein O-linked glycosylation via mannose / skeletal muscle fiber differentiation / basement membrane organization / dystroglycan binding / glial cell differentiation / reelin-mediated signaling pathway / skeletal muscle tissue regeneration / respiratory system process / protein import / camera-type eye development / adult walking behavior / response to alcohol / neuromuscular process / bone mineralization / skeletal muscle myofibril / heart morphogenesis / laminin binding / rough endoplasmic reticulum / response to glucocorticoid / muscle contraction / glycolytic process / response to activity / protein tetramerization / phosphatidylinositol 3-kinase/protein kinase B signal transduction / brain development / sarcolemma / protein processing / lipid metabolic process / neuron migration / in utero embryonic development / response to xenobiotic stimulus / inflammatory response / Golgi membrane / Golgi apparatus / extracellular space / nucleoplasm / metal ion binding / identical protein binding / cytosol
Similarity search - Function
LicD family / : / : / LicD family / Fukutin-related protein stem domain
Similarity search - Domain/homology
Ribitol 5-phosphate transferase FKRP
Similarity search - Component
Biological speciesHomo sapiens (human)
MethodX-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2.41 Å
AuthorsKuwabara, N.
Funding support Japan, 2items
OrganizationGrant numberCountry
Japan Society for the Promotion of Science16K07284 Japan
Japan Society for the Promotion of Science26840029 Japan
CitationJournal: Nat Commun / Year: 2020
Title: Crystal structures of fukutin-related protein (FKRP), a ribitol-phosphate transferase related to muscular dystrophy.
Authors: Kuwabara, N. / Imae, R. / Manya, H. / Tanaka, T. / Mizuno, M. / Tsumoto, H. / Kanagawa, M. / Kobayashi, K. / Toda, T. / Senda, T. / Endo, T. / Kato, R.
History
DepositionNov 3, 2019Deposition site: PDBJ / Processing site: PDBJ
Revision 1.0Jan 15, 2020Provider: repository / Type: Initial release
Revision 1.1Jan 29, 2020Group: Database references / Category: citation / citation_author
Item: _citation.journal_volume / _citation.page_first ..._citation.journal_volume / _citation.page_first / _citation.page_last / _citation.pdbx_database_id_PubMed / _citation.title / _citation_author.identifier_ORCID / _citation_author.name
Revision 1.2Jul 29, 2020Group: Data collection / Derived calculations / Structure summary
Category: chem_comp / entity ...chem_comp / entity / pdbx_chem_comp_identifier / pdbx_entity_nonpoly / pdbx_struct_conn_angle / struct_conn / struct_site / struct_site_gen
Item: _chem_comp.name / _chem_comp.type ..._chem_comp.name / _chem_comp.type / _entity.pdbx_description / _pdbx_entity_nonpoly.name / _pdbx_struct_conn_angle.ptnr1_auth_comp_id / _pdbx_struct_conn_angle.ptnr1_auth_seq_id / _pdbx_struct_conn_angle.ptnr1_label_asym_id / _pdbx_struct_conn_angle.ptnr1_label_atom_id / _pdbx_struct_conn_angle.ptnr1_label_comp_id / _pdbx_struct_conn_angle.ptnr1_label_seq_id / _pdbx_struct_conn_angle.ptnr2_auth_seq_id / _pdbx_struct_conn_angle.ptnr2_label_asym_id / _pdbx_struct_conn_angle.ptnr3_auth_comp_id / _pdbx_struct_conn_angle.ptnr3_auth_seq_id / _pdbx_struct_conn_angle.ptnr3_label_asym_id / _pdbx_struct_conn_angle.ptnr3_label_atom_id / _pdbx_struct_conn_angle.ptnr3_label_comp_id / _pdbx_struct_conn_angle.ptnr3_label_seq_id / _pdbx_struct_conn_angle.value / _struct_conn.conn_type_id / _struct_conn.id / _struct_conn.pdbx_dist_value / _struct_conn.pdbx_leaving_atom_flag / _struct_conn.pdbx_role / _struct_conn.ptnr1_auth_asym_id / _struct_conn.ptnr1_auth_comp_id / _struct_conn.ptnr1_auth_seq_id / _struct_conn.ptnr1_label_asym_id / _struct_conn.ptnr1_label_atom_id / _struct_conn.ptnr1_label_comp_id / _struct_conn.ptnr1_label_seq_id / _struct_conn.ptnr2_auth_asym_id / _struct_conn.ptnr2_auth_comp_id / _struct_conn.ptnr2_auth_seq_id / _struct_conn.ptnr2_label_asym_id / _struct_conn.ptnr2_label_atom_id / _struct_conn.ptnr2_label_comp_id
Description: Carbohydrate remediation / Provider: repository / Type: Remediation
Revision 1.3Nov 22, 2023Group: Data collection / Database references ...Data collection / Database references / Refinement description / Structure summary
Category: chem_comp / chem_comp_atom ...chem_comp / chem_comp_atom / chem_comp_bond / database_2 / pdbx_initial_refinement_model
Item: _chem_comp.pdbx_synonyms / _database_2.pdbx_DOI / _database_2.pdbx_database_accession
Revision 1.4Nov 13, 2024Group: Structure summary / Category: pdbx_entry_details / pdbx_modification_feature / Item: _pdbx_entry_details.has_protein_modification

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
A: Fukutin-related protein
B: Fukutin-related protein
C: Fukutin-related protein
D: Fukutin-related protein
hetero molecules


Theoretical massNumber of molelcules
Total (without water)202,06321
Polymers200,1074
Non-polymers1,95617
Water15,385854
1


  • Idetical with deposited unit
  • defined by author&software
  • Evidence: SAXS
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area12160 Å2
ΔGint-61 kcal/mol
Surface area75160 Å2
MethodPISA
Unit cell
Length a, b, c (Å)76.939, 119.384, 257.610
Angle α, β, γ (deg.)90.000, 90.000, 90.000
Int Tables number19
Space group name H-MP212121
Space group name HallP2ac2ab
Symmetry operation#1: x,y,z
#2: x+1/2,-y+1/2,-z
#3: -x,y+1/2,-z+1/2
#4: -x+1/2,-y,z+1/2

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Components

#1: Protein
Fukutin-related protein / Ribitol-5-phosphate transferase


Mass: 50026.805 Da / Num. of mol.: 4
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Gene: FKRP / Cell line (production host): HEK293S GnT- / Production host: Homo sapiens (human)
References: UniProt: Q9H9S5, Transferases; Glycosyltransferases; Pentosyltransferases
#2: Chemical
ChemComp-ZN / ZINC ION


Mass: 65.409 Da / Num. of mol.: 4 / Source method: obtained synthetically / Formula: Zn / Feature type: SUBJECT OF INVESTIGATION
#3: Sugar
ChemComp-NAG / 2-acetamido-2-deoxy-beta-D-glucopyranose / N-acetyl-beta-D-glucosamine / 2-acetamido-2-deoxy-beta-D-glucose / 2-acetamido-2-deoxy-D-glucose / 2-acetamido-2-deoxy-glucose / N-ACETYL-D-GLUCOSAMINE


Type: D-saccharide, beta linking / Mass: 221.208 Da / Num. of mol.: 7
Source method: isolated from a genetically manipulated source
Formula: C8H15NO6
IdentifierTypeProgram
DGlcpNAcbCONDENSED IUPAC CARBOHYDRATE SYMBOLGMML 1.0
N-acetyl-b-D-glucopyranosamineCOMMON NAMEGMML 1.0
b-D-GlcpNAcIUPAC CARBOHYDRATE SYMBOLPDB-CARE 1.0
GlcNAcSNFG CARBOHYDRATE SYMBOLGMML 1.0
#4: Chemical
ChemComp-MG / MAGNESIUM ION


Mass: 24.305 Da / Num. of mol.: 6 / Source method: obtained synthetically / Formula: Mg / Feature type: SUBJECT OF INVESTIGATION
#5: Water ChemComp-HOH / water


Mass: 18.015 Da / Num. of mol.: 854 / Source method: isolated from a natural source / Formula: H2O
Has ligand of interestY
Has protein modificationY

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Experimental details

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Experiment

ExperimentMethod: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

CrystalDensity Matthews: 3.05 Å3/Da / Density % sol: 59.66 %
Crystal growTemperature: 293 K / Method: vapor diffusion, hanging drop / pH: 7.5
Details: 4-8% PEG 4,000, 0.1 M HEPES-NaOH (pH7.5), and 0.2 M MgCl2

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Data collection

DiffractionMean temperature: 100 K / Serial crystal experiment: N
Diffraction sourceSource: SYNCHROTRON / Site: Photon Factory / Beamline: BL-17A / Wavelength: 1.2825 Å
DetectorType: DECTRIS PILATUS3 S 6M / Detector: PIXEL / Date: Jun 6, 2015
RadiationProtocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelengthWavelength: 1.2825 Å / Relative weight: 1
ReflectionResolution: 2.41→47.3 Å / Num. obs: 177276 / % possible obs: 100 % / Redundancy: 3.4 % / Biso Wilson estimate: 38.25 Å2 / CC1/2: 0.998 / Rmerge(I) obs: 0.074 / Rrim(I) all: 0.088 / Net I/σ(I): 12.97
Reflection shellResolution: 2.41→2.45 Å / Rmerge(I) obs: 0.631 / Mean I/σ(I) obs: 2.9 / Num. unique obs: 4418 / CC1/2: 0.839 / Rrim(I) all: 0.695

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Processing

Software
NameVersionClassification
PHENIX1.13_2998refinement
PHENIX1.13_2998refinement
XDSdata reduction
Aimlessdata scaling
PHASERphasing
RefinementMethod to determine structure: MOLECULAR REPLACEMENT
Starting model: 6KAN

6kan


Resolution: 2.41→47.3 Å / SU ML: 0.2726 / Cross valid method: FREE R-VALUE / σ(F): 1.34 / Phase error: 21.4418 / Stereochemistry target values: GeoStd + Monomer Library
RfactorNum. reflection% reflection
Rfree0.2108 8777 4.95 %
Rwork0.1817 168447 -
obs0.1831 177224 99.58 %
Solvent computationShrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL
Displacement parametersBiso mean: 49.49 Å2
Refinement stepCycle: LAST / Resolution: 2.41→47.3 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms13555 0 108 854 14517
Refine LS restraints
Refine-IDTypeDev idealNumber
X-RAY DIFFRACTIONf_bond_d0.007414063
X-RAY DIFFRACTIONf_angle_d0.781619277
X-RAY DIFFRACTIONf_chiral_restr0.04432148
X-RAY DIFFRACTIONf_plane_restr0.00542548
X-RAY DIFFRACTIONf_dihedral_angle_d8.20049037
LS refinement shell
Resolution (Å)Rfactor RfreeNum. reflection RfreeRfactor RworkNum. reflection RworkRefine-ID% reflection obs (%)
2.41-2.430.31733230.28625230X-RAY DIFFRACTION95.13
2.43-2.460.27973250.25265580X-RAY DIFFRACTION99.78
2.46-2.490.27452820.24125724X-RAY DIFFRACTION99.82
2.49-2.520.24243010.23825544X-RAY DIFFRACTION99.83
2.52-2.560.28342950.23975637X-RAY DIFFRACTION99.88
2.56-2.590.25442920.22875646X-RAY DIFFRACTION99.97
2.59-2.630.28133070.22065645X-RAY DIFFRACTION99.93
2.63-2.670.24452980.22455641X-RAY DIFFRACTION99.8
2.67-2.710.24023010.22075530X-RAY DIFFRACTION99.47
2.71-2.750.24342770.20555711X-RAY DIFFRACTION99.9
2.75-2.80.24172780.2095610X-RAY DIFFRACTION99.83
2.8-2.850.26452880.20225671X-RAY DIFFRACTION99.87
2.85-2.910.22822980.20145614X-RAY DIFFRACTION99.73
2.91-2.970.24752840.20335622X-RAY DIFFRACTION99.53
2.97-3.030.27043030.21645603X-RAY DIFFRACTION99.75
3.03-3.10.23282820.21545687X-RAY DIFFRACTION99.92
3.1-3.180.24412970.19865584X-RAY DIFFRACTION99.97
3.18-3.260.20842450.19775713X-RAY DIFFRACTION99.97
3.26-3.360.24853040.18665662X-RAY DIFFRACTION99.72
3.36-3.470.22672980.18935547X-RAY DIFFRACTION99.46
3.47-3.590.2152970.18475649X-RAY DIFFRACTION99.8
3.59-3.740.18642400.17225688X-RAY DIFFRACTION99.33
3.74-3.910.17913010.1575514X-RAY DIFFRACTION99.06
3.91-4.110.16992690.15075664X-RAY DIFFRACTION99.51
4.11-4.370.18363040.15135607X-RAY DIFFRACTION99.93
4.37-4.710.1583120.14155610X-RAY DIFFRACTION99.93
4.71-5.180.21262790.14295676X-RAY DIFFRACTION99.85
5.18-5.930.19632700.16945657X-RAY DIFFRACTION99.68
5.93-7.460.20363130.17425629X-RAY DIFFRACTION99.88
7.46-47.30.14963140.15665552X-RAY DIFFRACTION99.22
Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

IDL11 (°2)L12 (°2)L13 (°2)L22 (°2)L23 (°2)L33 (°2)S11 (Å °)S12 (Å °)S13 (Å °)S21 (Å °)S22 (Å °)S23 (Å °)S31 (Å °)S32 (Å °)S33 (Å °)T11 (Å2)T12 (Å2)T13 (Å2)T22 (Å2)T23 (Å2)T33 (Å2)Origin x (Å)Origin y (Å)Origin z (Å)
13.97203060234-0.8283391026960.7865224430945.00680056925-0.9211830353873.82760528464-0.2147356192920.05619775683260.0949420616337-0.0650412378260.006467621965160.6926690535030.130185132686-0.8079729391570.1094475979590.219804399007-0.0157360564021-0.04726720641070.363797425739-0.04795643279720.327332598644-13.7595636475237.97663858229.5861887138
21.099729173570.895682952174-0.1531091354113.209307855710.3850369597461.96635906584-0.01121257366660.16745176762-0.045816536412-0.4211207636810.01113284835680.3586849479180.0861439560007-0.1938178838980.005211146247740.3104271131730.0271729686014-0.05926338687580.31833893440.00361255007350.305216462006-6.91015701533239.04197529725.3587017499
32.413918158170.247468777413-0.01312460365721.925959607490.2005367664781.521144883580.0474950355232-0.291942130360.1570975554420.107634605899-0.1174639067530.388534815089-0.0422187574186-0.385501902760.07077581018110.2285102174390.02972915076390.05447182273030.313033880792-0.024249841890.282552925428-6.97192730477247.09087425142.9307228095
43.322113724351.982641857071.249001253172.630646157120.8864941704561.119407708290.0649476525466-0.346673328714-0.3882060500040.266222608222-0.0758805735029-0.1368704106450.2185113796-0.2385200846590.05497706754260.3642063433410.02250874090080.04436297407890.3527261944870.04701659610390.2837400112574.87264841763234.34661020747.9028462857
54.04094788466-0.5267179604620.1270016700976.87964610497-1.317056794375.65099024167-0.04589099245250.153486710169-0.0114418272522-0.172475771317-0.0171994480942-0.6480393160430.1437685702320.2772974893830.03421639841310.2284893586060.0330399684699-0.04759979009280.2684470841150.004830571782690.26214491339224.7209762139244.15499500748.1650384561
62.43128523009-1.462889109841.614340818173.56550740862-0.04980739178283.888332950470.0635445931344-0.142055751479-0.06109757869050.5963864937120.0189921077157-0.7319061307210.5461678970360.421193062112-0.07162267724690.4059898588060.0601536559753-0.1089156037610.3604043209270.02819761111020.38551355905931.6221509206239.25439143264.2907243954
72.695956557771.284729508152.083251011462.599977076661.718874775973.09214611251-0.5584459991550.1275552011110.2740306153250.3766553514570.681598222248-1.74734014965-0.6579095395311.29011357747-0.1098008016990.654749224661-0.0139920645419-0.3793765743760.681839733084-0.07003390464561.0729032383643.1901380577249.53981083269.255461406
82.372862430070.003808694380461.135704256233.31650894411-0.3423699419894.47716015145-0.165349065317-0.1694487920980.5032099235580.641969073470.00558706959534-0.634046779822-0.5446846208380.1287258615310.2472014319350.4847511477950.0304593184192-0.1239711687630.342904848853-0.02659554868460.48737019380528.5989329417256.43195909964.6847214492
91.850097857680.7007290787950.2048353583412.251158561070.5900010515161.71633698652-0.02571158399010.280284294103-0.26285233069-0.3575035218170.140555907251-0.5779299722660.07742768704660.300675742127-0.1212743787040.3620446284140.02271108352550.09423448517380.291341545278-0.03152437616660.36767492083624.3499636003244.1363501618.0870868828
100.9560628825871.02997456249-0.3142343835629.198543741540.3397665798341.392335010720.146264727132-0.140595912718-0.1083097749990.252735992498-0.0711169376658-0.769364080223-0.01668532475520.117697655025-0.04571114579690.4102412255890.0219010345132-0.1056038253650.262681219884-0.02543594826850.35268003459210.0616516512214.30817851418.4401192162
112.5420507923-0.4784278569821.489869730075.03062910912-1.178488014315.697424442970.0294860055483-0.254069962481-0.3133246604250.08299781763490.1030985253890.6189938657340.355011985933-0.605881033739-0.138957213640.392744433061-0.0188411203604-0.02231603947360.2740570451990.01469930780740.453747925369-1.07345219444199.58140693115.8705965983
123.15738743441-0.558427290943-0.613297477034.165881844020.7292622597153.43609635526-0.154909879942-0.107622589415-0.149264187138-0.234734308007-0.0601635176026-0.737628951345-0.2450485112210.8587622623110.1856655290040.265124814275-0.02884974515390.04551865060420.4048979408820.1067019638560.4429172179351.5925084915283.02820024130.4696363993
130.7062888235360.2227679788580.4307762163373.50439487231-0.3608229286881.73716417770.04644668496850.155983549365-0.0367201863633-0.398334209921-0.1854752152-0.5205045960440.06414159051180.3804637058490.1247530071920.2892579668860.04600488241450.07505102039460.3731400425310.02451340780410.33259618622844.6224626934282.09582051226.2547779623
143.532836981940.625486268492-0.7047962035732.96946683682-0.9998826153142.26117667562-0.00287555792829-0.147525177854-0.2666208923940.153157918315-0.216172428723-0.7920778863420.07420706920030.6000656540810.2068431061610.2265246432560.0412151698125-0.05783998738680.4035048916390.05889312800660.4714324962449.8910521611271.00716198343.6034364977
152.36450746080.419435532893-0.4975701427022.02914646648-0.7549441376231.37014651596-0.0160596454468-0.248308808203-0.09944299071140.0844447302693-0.206998873944-0.452352454751-0.03919343304090.3023657037470.2333617972440.2104406375010.0210973324622-0.04362764963870.3157450397770.02226653354560.30190287365242.6138125422274.2929518443.3329456691
161.764431213141.29934910834-0.8123941123093.01138442883-1.090317108521.22843950273-0.0831131327994-0.2194206656480.2875096859280.348150337871-0.104072994558-0.157634977803-0.2990870670250.142523041090.1960461475740.3431590833590.0283252917907-0.106939097540.351400117371-0.05274409386150.29757822564933.3171774827285.97834233349.093691242
171.42238866379-0.645579694269-1.681891233822.982627868510.4090632923294.171237122050.00836909671245-0.07830296180140.02699231777310.368194198592-0.0199009175140.346590722385-0.115751269652-0.3482000696910.01462932854770.2449719449430.02589020614470.02610838995750.308260478308-0.02759781631140.25251417879810.526408847276.47692790961.6936345271
182.941627524290.355141740857-2.150110042854.33344785242-1.01533875695.34061803973-0.0748901923293-0.0635517899018-0.03267708311970.311848668579-0.0150022536750.851490915337-0.160331973294-0.8888722693150.007239766645680.3809571235830.11335566270.08038838999070.521819009816-0.07132148196140.471876184409-0.325430317677281.74028433465.2128823965
191.566778722290.633398978548-0.6928611306923.160800767671.203742037964.32470536986-0.0528910133052-0.214561295052-0.3105051609840.796590331932-0.07406147273140.5064395263620.501129895415-0.2923879041750.2065876200970.4419166555830.03127265622790.1135554473930.3820779553590.02121160946770.4073743038218.60316322689264.13095162567.0523680743
205.41871923403-0.354405471898-0.2628347027167.861581576981.88941516176.8280173076-0.079579779210.273637404231-0.110878736498-0.06209318541820.2261196175020.7908525848810.289715287248-0.483458181466-0.1060551804280.249006717555-0.016544824919-0.05896125180740.2746099077550.07579973634780.2369359769717.72140464415270.50476029224.222443391
211.54968032958-1.0454244270.069347310250.9176962683260.2118617449680.797573225197-0.164421166646-0.0722103350170.103008159904-0.07003544231690.0966890616173-0.0298871020543-0.1674424065630.0514183158220.1055619046320.283796384371-0.033070016323-0.009876924647690.222623047215-0.04776368553580.32829489506216.3810475984269.74193930133.8820311834
221.21747771683-0.8309296232580.5441386715363.34493733272-0.2034537899785.958740465950.04475298271750.600219690336-0.239379376264-0.704767018831-0.02404461096670.3597625052970.289633533189-0.300783005999-0.01601139431060.410246316348-0.0528943332325-0.08204890710780.297572903569-0.03111282228720.30337350450812.8272274504270.05941402215.6230250862
233.233657198982.129468724581.429914830793.409994078270.1357307439361.64054205421-0.3479807716420.5225509670840.369403436666-0.8087406698350.3427735156050.46371190805-0.213881321204-0.0931574219960.00816028824470.5787385139380.00699992768084-0.1440102711070.4003394330640.05520781223650.33777693950614.4813353785288.85351632110.4267558076
243.032575360830.606506392788-0.5457351671492.94154324833-1.107226650462.74214176314-0.1564040245890.321637662610.132164259166-0.5073951182110.1736927264420.2815438046770.00864542825106-0.3003032082730.02311584693170.3968352843150.0226505010582-0.09434201985840.2536276373680.005845580833960.23280111694513.752498911279.95837754715.9455381471
255.574511275495.04450612161-6.93480968546.73878717519-4.785538765382.840514880260.854974760138-0.248495868050.785702721490.868512587721-0.2296299661770.694895446728-0.572151055579-0.11855256607-0.5951954611640.5000604193240.02736855095030.01462933813260.425454173105-0.01771610418730.37608584995111.8525275918291.36086808329.8853816678
266.39046718309-0.09769803217790.3191250198473.77618428218-0.4086954187451.374796948040.174408935701-0.217219712217-0.01011362861310.172944872747-0.0571535182364-0.0349918072619-0.05536983740590.0241674694362-0.1295685481260.493596854332-0.000281781574614-0.006614936377420.2417771196960.04968409918760.25806167383728.7824962015302.25878197622.0380206512
271.724906787770.266065705017-0.6718963401843.26526220817-1.15348333483.264141204660.342124164358-0.3552468570780.4111174566670.503432683816-0.17157372583-0.303090459157-0.8272714647860.731758550094-0.1557921178280.640509740864-0.208959406320.03892538178050.413699170271-0.06855487469180.40727936487735.4860865696322.43661703119.6211549513
Refinement TLS group
IDRefine-IDRefine TLS-IDSelection details
1X-RAY DIFFRACTION1chain 'A' and (resid 46 through 83 )
2X-RAY DIFFRACTION2chain 'A' and (resid 84 through 139 )
3X-RAY DIFFRACTION3chain 'A' and (resid 140 through 250 )
4X-RAY DIFFRACTION4chain 'A' and (resid 251 through 289 )
5X-RAY DIFFRACTION5chain 'A' and (resid 290 through 315 )
6X-RAY DIFFRACTION6chain 'A' and (resid 316 through 413 )
7X-RAY DIFFRACTION7chain 'A' and (resid 414 through 446 )
8X-RAY DIFFRACTION8chain 'A' and (resid 447 through 493 )
9X-RAY DIFFRACTION9chain 'B' and (resid 46 through 272 )
10X-RAY DIFFRACTION10chain 'B' and (resid 273 through 336 )
11X-RAY DIFFRACTION11chain 'B' and (resid 337 through 490 )
12X-RAY DIFFRACTION12chain 'C' and (resid 47 through 83 )
13X-RAY DIFFRACTION13chain 'C' and (resid 84 through 139 )
14X-RAY DIFFRACTION14chain 'C' and (resid 140 through 174 )
15X-RAY DIFFRACTION15chain 'C' and (resid 175 through 250 )
16X-RAY DIFFRACTION16chain 'C' and (resid 251 through 289 )
17X-RAY DIFFRACTION17chain 'C' and (resid 290 through 374 )
18X-RAY DIFFRACTION18chain 'C' and (resid 375 through 442 )
19X-RAY DIFFRACTION19chain 'C' and (resid 443 through 493 )
20X-RAY DIFFRACTION20chain 'D' and (resid 47 through 83 )
21X-RAY DIFFRACTION21chain 'D' and (resid 84 through 111 )
22X-RAY DIFFRACTION22chain 'D' and (resid 112 through 150 )
23X-RAY DIFFRACTION23chain 'D' and (resid 151 through 197 )
24X-RAY DIFFRACTION24chain 'D' and (resid 198 through 250 )
25X-RAY DIFFRACTION25chain 'D' and (resid 251 through 272 )
26X-RAY DIFFRACTION26chain 'D' and (resid 273 through 315 )
27X-RAY DIFFRACTION27chain 'D' and (resid 316 through 485 )

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