+Open data
-Basic information
Entry | Database: PDB / ID: 6l7r | ||||||
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Title | Crystal structure of Chaetomium GCP3 N-terminus and Mozart1 | ||||||
Components |
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Keywords | TRANSLATION / gamma tubulin complex / microprotien / microtubule | ||||||
Function / homology | Function and homology information gamma-tubulin complex localization / gamma-tubulin ring complex / microtubule nucleation / spindle pole body / gamma-tubulin binding / microtubule organizing center / spindle pole / microtubule / cytoplasm Similarity search - Function | ||||||
Biological species | Chaetomium thermophilum (fungus) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / SAD / Resolution: 1.84813131157 Å | ||||||
Authors | Huang, T.L. / Wang, H.J. / Hsia, K.C. | ||||||
Funding support | Taiwan, 1items
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Citation | Journal: Cell Rep / Year: 2020 Title: Promiscuous Binding of Microprotein Mozart1 to gamma-Tubulin Complex Mediates Specific Subcellular Targeting to Control Microtubule Array Formation. Authors: Huang, T.L. / Wang, H.J. / Chang, Y.C. / Wang, S.W. / Hsia, K.C. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 6l7r.cif.gz | 104.8 KB | Display | PDBx/mmCIF format |
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PDB format | pdb6l7r.ent.gz | 66.5 KB | Display | PDB format |
PDBx/mmJSON format | 6l7r.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/l7/6l7r ftp://data.pdbj.org/pub/pdb/validation_reports/l7/6l7r | HTTPS FTP |
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-Related structure data
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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Components on special symmetry positions |
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-Components
#1: Protein | Mass: 11821.466 Da / Num. of mol.: 1 / Fragment: N-terminus Source method: isolated from a genetically manipulated source Source: (gene. exp.) Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719) (fungus) Strain: DSM 1495 / CBS 144.50 / IMI 039719 / Gene: CTHT_0063320 / Production host: Escherichia coli (E. coli) / References: UniProt: G0SED1 |
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#2: Protein | Mass: 9492.858 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719) (fungus) Strain: DSM 1495 / CBS 144.50 / IMI 039719 / Gene: CTHT_0002490 / Production host: Escherichia coli (E. coli) / References: UniProt: G0RZC6 |
#3: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.3 Å3/Da / Density % sol: 46.64 % |
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Crystal grow | Temperature: 293 K / Method: vapor diffusion, hanging drop / pH: 8.5 Details: 0.2 M lithium sulfate monohydrate, 0.1 M Tris hydrochloride pH 8.5, 30% PEG 4000 |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N |
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Diffraction source | Source: SYNCHROTRON / Site: NSRRC / Beamline: TPS 05A / Wavelength: 0.9784 Å |
Detector | Type: RAYONIX MX300-HS / Detector: CCD / Date: Mar 6, 2018 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.9784 Å / Relative weight: 1 |
Reflection | Resolution: 1.84→20 Å / Num. obs: 101437 / % possible obs: 99.5 % / Redundancy: 6 % / Biso Wilson estimate: 29.5273447895 Å2 / CC1/2: 0.995 / Net I/σ(I): 26.1 |
Reflection shell | Resolution: 1.84→1.92 Å / Num. unique obs: 1621 / CC1/2: 0.973 |
-Processing
Software |
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Refinement | Method to determine structure: SAD / Resolution: 1.84813131157→19.9518531996 Å / SU ML: 0.193769979505 / Cross valid method: FREE R-VALUE / σ(F): 1.40459111588 / Phase error: 24.0380271756 Stereochemistry target values: GeoStd + Monomer Library + CDL v1.2
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 39.1126660995 Å2 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 1.84813131157→19.9518531996 Å
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Refine LS restraints |
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LS refinement shell |
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Refinement TLS params. | Method: refined / Origin x: -19.1395126694 Å / Origin y: 8.83757201141 Å / Origin z: 9.59516055196 Å
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Refinement TLS group | Selection details: all |