+Open data
-Basic information
Entry | Database: PDB / ID: 6l82 | ||||||
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Title | Crystal structure of Chaetomium GCP5 N-terminus and Mozart1 | ||||||
Components |
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Keywords | TRANSLATION / gamma tubulin complex / microprotein / microtubule | ||||||
Function / homology | Function and homology information gamma-tubulin complex localization / gamma-tubulin ring complex / microtubule nucleation / spindle pole body / gamma-tubulin binding / microtubule organizing center / spindle pole / microtubule / cytoplasm Similarity search - Function | ||||||
Biological species | Chaetomium thermophilum (fungus) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2.24103629119 Å | ||||||
Authors | Huang, T.L. / Wang, H.J. / Wang, S.W. / Hsia, K.C. | ||||||
Funding support | Taiwan, 1items
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Citation | Journal: Cell Rep / Year: 2020 Title: Promiscuous Binding of Microprotein Mozart1 to gamma-Tubulin Complex Mediates Specific Subcellular Targeting to Control Microtubule Array Formation. Authors: Huang, T.L. / Wang, H.J. / Chang, Y.C. / Wang, S.W. / Hsia, K.C. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 6l82.cif.gz | 93.5 KB | Display | PDBx/mmCIF format |
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PDB format | pdb6l82.ent.gz | 58.9 KB | Display | PDB format |
PDBx/mmJSON format | 6l82.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/l8/6l82 ftp://data.pdbj.org/pub/pdb/validation_reports/l8/6l82 | HTTPS FTP |
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-Related structure data
Related structure data | 6l7rSC 6l80C 6l81C S: Starting model for refinement C: citing same article (ref.) |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
#1: Protein | Mass: 12204.667 Da / Num. of mol.: 1 / Fragment: N-terminus Source method: isolated from a genetically manipulated source Source: (gene. exp.) Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719) (fungus) Strain: DSM 1495 / CBS 144.50 / IMI 039719 / Gene: CTHT_0065630 / Production host: Escherichia coli (E. coli) / References: UniProt: G0SGA5 |
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#2: Protein | Mass: 9492.858 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719) (fungus) Strain: DSM 1495 / CBS 144.50 / IMI 039719 / Gene: CTHT_0002490 / Production host: Escherichia coli (E. coli) / References: UniProt: G0RZC6 |
#3: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.54 Å3/Da / Density % sol: 51.58 % |
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Crystal grow | Temperature: 293 K / Method: vapor diffusion, hanging drop / pH: 7 Details: 1% tryptone, 0.05 M HEPES sodium pH 7.0, 0.001 M sodium azide, 20% PEG 3350 |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N |
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Diffraction source | Source: SYNCHROTRON / Site: NSRRC / Beamline: TPS 05A / Wavelength: 0.9998 Å |
Detector | Type: RAYONIX MX300-HS / Detector: CCD / Date: Nov 16, 2018 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.9998 Å / Relative weight: 1 |
Reflection | Resolution: 2.2→20 Å / Num. obs: 77073 / % possible obs: 97.3 % / Redundancy: 7.2 % / Biso Wilson estimate: 32.8744335331 Å2 / CC1/2: 0.997 / Net I/σ(I): 14.2 |
Reflection shell | Resolution: 2.25→2.33 Å / Num. unique obs: 10652 / CC1/2: 0.923 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: 6L7R Resolution: 2.24103629119→19.9026490275 Å / SU ML: 0.244501859732 / Cross valid method: FREE R-VALUE / σ(F): 1.3718047686 / Phase error: 27.2376552466
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 44.6940107881 Å2 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.24103629119→19.9026490275 Å
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Refine LS restraints |
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LS refinement shell |
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Refinement TLS params. | Method: refined / Origin x: 2.59473814634 Å / Origin y: 17.0808025721 Å / Origin z: -0.935803772826 Å
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Refinement TLS group | Selection details: all |