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Open data
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Basic information
Entry | Database: PDB / ID: 6l6m | ||||||
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Title | HSP18.5 from E. histolytica | ||||||
![]() | Heat shock protein hsp20 family putative | ||||||
![]() | CHAPERONE / Small heat shock protein HSP18.5 Molecular chaperone E. histolytica | ||||||
Function / homology | Hsp20/alpha crystallin family / Small heat shock protein (sHSP) domain profile. / Alpha crystallin/Hsp20 domain / HSP20-like chaperone / Heat shock protein, Hsp20 family, putative![]() | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Kurre, D. / Suguna, K. | ||||||
Funding support | ![]()
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![]() | ![]() Title: Network of Entamoeba histolytica HSP18.5 dimers formed by two overlapping [IV]-X-[IV] motifs. Authors: Kurre, D. / Suguna, K. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 116.3 KB | Display | ![]() |
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PDB format | ![]() | 71.6 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 452.3 KB | Display | ![]() |
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Full document | ![]() | 458.1 KB | Display | |
Data in XML | ![]() | 16.1 KB | Display | |
Data in CIF | ![]() | 21.6 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 3w1zS S: Starting model for refinement |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 18848.393 Da / Num. of mol.: 4 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 3.32 Å3/Da / Density % sol: 65.92 % |
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Crystal grow | Temperature: 295 K / Method: vapor diffusion, hanging drop / pH: 6.2 Details: 0.1 M Bis-Tris 160 mM ammonium acetate 45% 2-Methyl-2,4-pentanediol PH range: 6.0-7.5 |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: DECTRIS PILATUS 6M / Detector: PIXEL / Date: Aug 31, 2017 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.991872 Å / Relative weight: 1 |
Reflection | Resolution: 3.28→79.53 Å / Num. obs: 15560 / % possible obs: 99.9 % / Redundancy: 11.1 % / Biso Wilson estimate: 112.375814491 Å2 / CC1/2: 0.99 / Net I/σ(I): 5.99 |
Reflection shell | Resolution: 3.28→3.43 Å / Num. unique obs: 2004 / CC1/2: 0.99 / % possible all: 99.9 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: 3W1Z Resolution: 3.28000381979→79.529 Å / SU ML: 0.359075090824 / Cross valid method: FREE R-VALUE / σ(F): 1.91706635515 / Phase error: 24.653739223 Stereochemistry target values: GeoStd + Monomer Library + CDL v1.2
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL | ||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 99.2352389117 Å2 | ||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 3.28000381979→79.529 Å
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Refine LS restraints |
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LS refinement shell |
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