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Yorodumi- PDB-6kh8: Solution structure of Zn free Bovine Pancreatic Insulin in 20% ac... -
+Open data
-Basic information
Entry | Database: PDB / ID: 6kh8 | |||||||||
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Title | Solution structure of Zn free Bovine Pancreatic Insulin in 20% acetic acid-d4 (pH 1.9) | |||||||||
Components |
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Keywords | HORMONE / Insulin / Fibrillation / Amyloid | |||||||||
Function / homology | Function and homology information estradiol secretion / negative regulation of lactation / positive regulation of blood circulation / glucose import in response to insulin stimulus / positive regulation of cell maturation / positive regulation of lactation / response to L-arginine / positive regulation of mammary gland epithelial cell proliferation / negative regulation of appetite / response to butyrate ...estradiol secretion / negative regulation of lactation / positive regulation of blood circulation / glucose import in response to insulin stimulus / positive regulation of cell maturation / positive regulation of lactation / response to L-arginine / positive regulation of mammary gland epithelial cell proliferation / negative regulation of appetite / response to butyrate / feeding behavior / response to growth hormone / response to food / positive regulation of Rho protein signal transduction / positive regulation of peptide hormone secretion / protein secretion / response to glucose / negative regulation of lipid catabolic process / response to nutrient levels / positive regulation of protein secretion / insulin receptor binding / hormone activity / positive regulation of insulin secretion / glucose metabolic process / glucose homeostasis / response to heat / positive regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction / positive regulation of gene expression / negative regulation of apoptotic process / extracellular space / identical protein binding Similarity search - Function | |||||||||
Biological species | Bos taurus (cattle) | |||||||||
Method | SOLUTION NMR / simulated annealing | |||||||||
Authors | Bhunia, A. / Ratha, B.N. / Kar, R.K. / Brender, J.R. | |||||||||
Funding support | India, 2items
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Citation | Journal: Proteins / Year: 2020 Title: High-resolution structure of a partially folded insulin aggregation intermediate. Authors: Ratha, B.N. / Kar, R.K. / Brender, J.R. / Pariary, R. / Sahoo, B. / Kalita, S. / Bhunia, A. | |||||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 6kh8.cif.gz | 154.7 KB | Display | PDBx/mmCIF format |
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PDB format | pdb6kh8.ent.gz | 134.4 KB | Display | PDB format |
PDBx/mmJSON format | 6kh8.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/kh/6kh8 ftp://data.pdbj.org/pub/pdb/validation_reports/kh/6kh8 | HTTPS FTP |
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-Related structure data
Related structure data | 6kh9C 6khaC C: citing same article (ref.) |
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Similar structure data | |
Other databases |
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-Links
-Assembly
Deposited unit |
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1 |
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NMR ensembles |
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-Components
#1: Protein/peptide | Mass: 2339.645 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Bos taurus (cattle) / References: UniProt: P01317 |
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#2: Protein/peptide | Mass: 3403.927 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Bos taurus (cattle) / References: UniProt: P01317 |
-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||||
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NMR experiment |
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-Sample preparation
Details | Type: solution Contents: 350 uM Bovine insulin Chain A, 350 uM Bovine Insulin chain B, 20% acetic acid-D4 Label: BPI 0h / Solvent system: 20% acetic acid-D4 | ||||||||||||
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Sample |
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Sample conditions | Ionic strength: 20% ACETIC ACID Not defined / Label: 1 / pH: 1.9 / Pressure: 1 atm / Temperature: 298 K |
-NMR measurement
NMR spectrometer | Type: Bruker AVANCE III / Manufacturer: Bruker / Model: AVANCE III / Field strength: 700 MHz |
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-Processing
NMR software |
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Refinement | Method: simulated annealing / Software ordinal: 5 | ||||||||||||||||||||||||
NMR representative | Selection criteria: lowest energy | ||||||||||||||||||||||||
NMR ensemble | Conformer selection criteria: structures with the lowest energy Conformers calculated total number: 100 / Conformers submitted total number: 10 |