+Open data
-Basic information
Entry | Database: PDB / ID: 6kh9 | ||||||
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Title | Solution structure of bovine insulin amyloid intermediate-1 | ||||||
Components |
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Keywords | HORMONE / diabetes / insulin | ||||||
Function / homology | Function and homology information estradiol secretion / negative regulation of lactation / positive regulation of blood circulation / glucose import in response to insulin stimulus / positive regulation of cell maturation / positive regulation of lactation / response to L-arginine / positive regulation of mammary gland epithelial cell proliferation / negative regulation of appetite / response to butyrate ...estradiol secretion / negative regulation of lactation / positive regulation of blood circulation / glucose import in response to insulin stimulus / positive regulation of cell maturation / positive regulation of lactation / response to L-arginine / positive regulation of mammary gland epithelial cell proliferation / negative regulation of appetite / response to butyrate / feeding behavior / response to growth hormone / response to food / positive regulation of Rho protein signal transduction / positive regulation of peptide hormone secretion / protein secretion / negative regulation of lipid catabolic process / response to glucose / response to nutrient levels / positive regulation of protein secretion / insulin receptor binding / positive regulation of insulin secretion / hormone activity / glucose metabolic process / glucose homeostasis / response to heat / positive regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction / positive regulation of gene expression / negative regulation of apoptotic process / extracellular space / identical protein binding Similarity search - Function | ||||||
Biological species | Bos taurus (cattle) | ||||||
Method | SOLUTION NMR / simulated annealing | ||||||
Authors | Ratha, B.N. / Kar, R.K. / Brender, J.B. / Bhunia, A. | ||||||
Funding support | India, 1items
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Citation | Journal: Proteins / Year: 2020 Title: High-resolution structure of a partially folded insulin aggregation intermediate. Authors: Ratha, B.N. / Kar, R.K. / Brender, J.R. / Pariary, R. / Sahoo, B. / Kalita, S. / Bhunia, A. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 6kh9.cif.gz | 155.9 KB | Display | PDBx/mmCIF format |
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PDB format | pdb6kh9.ent.gz | 135.3 KB | Display | PDB format |
PDBx/mmJSON format | 6kh9.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 6kh9_validation.pdf.gz | 391.7 KB | Display | wwPDB validaton report |
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Full document | 6kh9_full_validation.pdf.gz | 482.9 KB | Display | |
Data in XML | 6kh9_validation.xml.gz | 10.4 KB | Display | |
Data in CIF | 6kh9_validation.cif.gz | 16.4 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/kh/6kh9 ftp://data.pdbj.org/pub/pdb/validation_reports/kh/6kh9 | HTTPS FTP |
-Related structure data
Related structure data | 6kh8C 6khaC C: citing same article (ref.) |
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Similar structure data | |
Other databases |
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-Links
-Assembly
Deposited unit |
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1 |
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NMR ensembles |
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-Components
#1: Protein/peptide | Mass: 2339.645 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Bos taurus (cattle) / References: UniProt: P01317 |
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#2: Protein/peptide | Mass: 3403.927 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Bos taurus (cattle) / References: UniProt: P01317 |
-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||||
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NMR experiment |
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-Sample preparation
Details | Type: solution / Contents: 350 uM Bovine insulin, 20% acetic acid-d4 Details: 350 uM bovine insulin in 20% acetic acid-d4 at pH 1.9 Label: BPI_18hr_D4Ac / Solvent system: 20% acetic acid-d4 |
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Sample | Conc.: 350 uM / Component: Bovine insulin / Isotopic labeling: none |
Sample conditions | Ionic strength: 20% Acetic acid-d4 Not defined / Label: BPI_18hr_D4Ac / pH: 1.9 / Pressure: ambient atm / Temperature: 298 K |
-NMR measurement
NMR spectrometer | Type: Bruker AVANCE III / Manufacturer: Bruker / Model: AVANCE III / Field strength: 700 MHz / Details: Bose Institute, Kolkata |
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-Processing
NMR software |
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Refinement | Method: simulated annealing / Software ordinal: 5 | ||||||||||||||||||||||||
NMR representative | Selection criteria: lowest energy | ||||||||||||||||||||||||
NMR ensemble | Conformer selection criteria: structures with the lowest energy Conformers calculated total number: 50 / Conformers submitted total number: 10 |