0.35 mM [U-99% 13C; U-99% 15N] DNA dC->dU-editing enzyme APOBEC3G, 0.525 mM DNA/RNA (5'-D(*AP*TP*TP*CP*UP*(ICY)P*AP*AP*TP*T)-3'), 50 mM sodium phosphate, 50 mM sodium chloride, 5 mM DTT, 50 uM ZINC ION, 90% H2O/10% D2O
13C,15N_sample
90% H2O/10% D2O
solution
2
0.35 mM [U-99% 13C; U-99% 15N] DNA dC->dU-editing enzyme APOBEC3G, 0.525 mM DNA/RNA (5'-D(*AP*TP*TP*CP*UP*(ICY)P*AP*AP*TP*T)-3'), 50 mM sodium phosphate, 50 mM sodium chloride, 5 mM DTT, 50 uM ZINC ION, 100% D2O
13C,15N_sample_2
100% D2O
solution
4
0.35 mM [U-100% 13C; U-100% 15N; U-80% 2H] DNA dC->dU-editing enzyme APOBEC3G, 0.525 mM DNA/RNA (5'-D(*AP*TP*TP*CP*UP*(ICY)P*AP*AP*TP*T)-3'), 50 mM sodium phosphate, 50 mM sodium chloride, 5 mM DTT, 50 uM ZINC ION, 90% H2O/10% D2O
13C,15N,2H_sample
90% H2O/10% D2O
solution
3
1 mM DNA/RNA (5'-D(*AP*TP*TP*CP*UP*(ICY)P*AP*AP*TP*T)-3'), 50 mM sodium phosphate, 50 mM sodium chloride, 50 uM ZINC ION, 90% H2O/10% D2O