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- PDB-6ju7: Aspergillus oryzae active-tyrosinase copper-depleted C92A mutant ... -

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Basic information

Entry
Database: PDB / ID: 6ju7
TitleAspergillus oryzae active-tyrosinase copper-depleted C92A mutant complexed with L-tyrosine
ComponentsTyrosinase
KeywordsOXIDOREDUCTASE / Tyrosinase / Copper enzyme / dinuclear copper center
Function / homology
Function and homology information


tyrosinase / tyrosinase activity / melanin biosynthetic process / metal ion binding / nucleus
Similarity search - Function
Domain of unknown function DUF1907 / DUF1907 / Tyosinase, C-terminal / Tyrosinase C-terminal domain / di-copper center containing domain from catechol oxidase / Di-copper center containing domain from catechol oxidase / Tyrosinase CuA-binding region signature. / : / Common central domain of tyrosinase / Tyrosinase and hemocyanins CuB-binding region signature. ...Domain of unknown function DUF1907 / DUF1907 / Tyosinase, C-terminal / Tyrosinase C-terminal domain / di-copper center containing domain from catechol oxidase / Di-copper center containing domain from catechol oxidase / Tyrosinase CuA-binding region signature. / : / Common central domain of tyrosinase / Tyrosinase and hemocyanins CuB-binding region signature. / Tyrosinase copper-binding domain / Di-copper centre-containing domain superfamily / Orthogonal Bundle / Mainly Alpha
Similarity search - Domain/homology
NITRATE ION / TYROSINE / tyrosinase
Similarity search - Component
Biological speciesAspergillus oryzae (mold)
MethodX-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 1.42 Å
AuthorsFujieda, N. / Umakoshi, K. / Nishikawa, Y. / Kurisu, G. / Itoh, S.
Funding support Japan, 1items
OrganizationGrant numberCountry
Japan Science and TechnologyCREST(JPMJCR16P1) Japan
CitationJournal: Angew.Chem.Int.Ed.Engl. / Year: 2020
Title: Copper-Oxygen Dynamics in the Tyrosinase Mechanism.
Authors: Fujieda, N. / Umakoshi, K. / Ochi, Y. / Nishikawa, Y. / Yanagisawa, S. / Kubo, M. / Kurisu, G. / Itoh, S.
History
DepositionApr 13, 2019Deposition site: PDBJ / Processing site: PDBJ
Revision 1.0May 13, 2020Provider: repository / Type: Initial release
Revision 1.1Aug 12, 2020Group: Database references / Category: citation / citation_author
Item: _citation.journal_volume / _citation.page_first ..._citation.journal_volume / _citation.page_first / _citation.page_last / _citation.title / _citation_author.identifier_ORCID
Revision 1.2Nov 22, 2023Group: Data collection / Database references / Refinement description
Category: chem_comp_atom / chem_comp_bond ...chem_comp_atom / chem_comp_bond / database_2 / pdbx_initial_refinement_model
Item: _database_2.pdbx_DOI / _database_2.pdbx_database_accession

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

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Assembly

Deposited unit
A: Tyrosinase
B: Tyrosinase
hetero molecules


Theoretical massNumber of molelcules
Total (without water)107,4165
Polymers106,9922
Non-polymers4243
Water15,259847
1


  • Idetical with deposited unit
  • defined by author&software
  • Evidence: gel filtration
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area3130 Å2
ΔGint-8 kcal/mol
Surface area33610 Å2
MethodPISA
Unit cell
Length a, b, c (Å)54.504, 104.896, 154.738
Angle α, β, γ (deg.)90.00, 90.00, 90.00
Int Tables number19
Space group name H-MP212121

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Components

#1: Protein Tyrosinase


Mass: 53495.855 Da / Num. of mol.: 2 / Mutation: C92A
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Aspergillus oryzae (mold) / Gene: OAory_01107480 / Production host: Escherichia coli (E. coli) / References: UniProt: A0A1S9DK56, tyrosinase
#2: Chemical ChemComp-TYR / TYROSINE


Type: L-peptide linking / Mass: 181.189 Da / Num. of mol.: 2 / Source method: obtained synthetically / Formula: C9H11NO3
#3: Chemical ChemComp-NO3 / NITRATE ION


Mass: 62.005 Da / Num. of mol.: 1 / Source method: obtained synthetically / Formula: NO3
#4: Water ChemComp-HOH / water


Mass: 18.015 Da / Num. of mol.: 847 / Source method: isolated from a natural source / Formula: H2O

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Experimental details

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Experiment

ExperimentMethod: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

CrystalDensity Matthews: 2.17 Å3/Da / Density % sol: 43.41 %
Crystal growTemperature: 293 K / Method: vapor diffusion, hanging drop / pH: 7.2 / Details: 20% polyethylene glycol (PEG) 3350, 30mM NH4NO3

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Data collection

DiffractionMean temperature: 100 K / Serial crystal experiment: N
Diffraction sourceSource: SYNCHROTRON / Site: SPring-8 / Beamline: BL44XU / Wavelength: 0.9 Å
DetectorType: RAYONIX MX300HE / Detector: CCD / Date: May 20, 2016
RadiationProtocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelengthWavelength: 0.9 Å / Relative weight: 1
ReflectionResolution: 1.42→50 Å / Num. obs: 167149 / % possible obs: 99.4 % / Redundancy: 6.6 % / Rmerge(I) obs: 0.087 / Net I/σ(I): 20.2
Reflection shellResolution: 1.42→1.44 Å / Redundancy: 6.1 % / Rmerge(I) obs: 0.43 / Mean I/σ(I) obs: 4.5 / Num. unique obs: 7469 / % possible all: 89.6

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Processing

Software
NameVersionClassification
DENZOdata reduction
HKL-2000data scaling
PHASERphasing
SHELXL2017/1refinement
RefinementMethod to determine structure: MOLECULAR REPLACEMENT
Starting model: 3W6W

3w6w


Resolution: 1.42→30 Å / Cross valid method: FREE R-VALUE / σ(F): 0
RfactorNum. reflection% reflectionSelection details
Rfree0.1945 8257 5 %RANDOM
Rwork0.1514 ---
obs0.1554 167034 99.3 %-
Refine analyzeOccupancy sum hydrogen: 0
Refinement stepCycle: LAST / Resolution: 1.42→30 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms7050 0 30 847 7927
Refine LS restraints
Refine-IDTypeDev ideal
X-RAY DIFFRACTIONs_bond_d0.0064
X-RAY DIFFRACTIONs_angle_d0.0196
X-RAY DIFFRACTIONs_similar_dist0
X-RAY DIFFRACTIONs_from_restr_planes0.3839
X-RAY DIFFRACTIONs_zero_chiral_vol0.0661
X-RAY DIFFRACTIONs_non_zero_chiral_vol0.0648
X-RAY DIFFRACTIONs_anti_bump_dis_restr0
X-RAY DIFFRACTIONs_rigid_bond_adp_cmpnt0.0257
X-RAY DIFFRACTIONs_similar_adp_cmpnt0.042
X-RAY DIFFRACTIONs_approx_iso_adps0

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