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Open data
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Basic information
Entry | Database: PDB / ID: 6ju6 | ||||||
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Title | Aspergillus oryzae active-tyrosinase copper-depleted C92A mutant | ||||||
![]() | Tyrosinase | ||||||
![]() | OXIDOREDUCTASE / Tyrosinase / Copper enzyme / dinuclear copper center | ||||||
Function / homology | ![]() tyrosinase / tyrosinase activity / melanin biosynthetic process / nucleus / metal ion binding Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Fujieda, N. / Umakoshi, K. / Nishikawa, Y. / Kurisu, G. / Itoh, S. | ||||||
Funding support | ![]()
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![]() | ![]() Title: Copper-Oxygen Dynamics in the Tyrosinase Mechanism. Authors: Fujieda, N. / Umakoshi, K. / Ochi, Y. / Nishikawa, Y. / Yanagisawa, S. / Kubo, M. / Kurisu, G. / Itoh, S. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 372.1 KB | Display | ![]() |
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PDB format | ![]() | 297.9 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 449.1 KB | Display | ![]() |
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Full document | ![]() | 453.9 KB | Display | |
Data in XML | ![]() | 38.3 KB | Display | |
Data in CIF | ![]() | 57.9 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 6ju4C ![]() 6ju5C ![]() 6ju7C ![]() 6ju8C ![]() 6ju9C ![]() 6juaC ![]() 6jubC ![]() 6jucC ![]() 6judC ![]() 3w6wS S: Starting model for refinement C: citing same article ( |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 53495.855 Da / Num. of mol.: 2 / Mutation: C92A Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() #2: Chemical | ChemComp-NO3 / | #3: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.11 Å3/Da / Density % sol: 41.87 % |
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Crystal grow | Temperature: 293 K / Method: vapor diffusion, hanging drop / pH: 7.2 / Details: 20% polyethylene glycol (PEG) 3350, 30mM NH4NO3 |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: RAYONIX MX300HE / Detector: CCD / Date: Jul 13, 2016 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.9 Å / Relative weight: 1 |
Reflection | Resolution: 1.5→50 Å / Num. obs: 141249 / % possible obs: 99.6 % / Redundancy: 7 % / Rmerge(I) obs: 0.075 / Net I/σ(I): 26.6 |
Reflection shell | Resolution: 1.5→1.53 Å / Redundancy: 6.6 % / Rmerge(I) obs: 0.55 / Mean I/σ(I) obs: 3.4 / Num. unique obs: 6905 / % possible all: 99.1 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: 3W6W Resolution: 1.5→30 Å / Cross valid method: FREE R-VALUE / σ(F): 0
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Refine analyze | Num. disordered residues: 36 / Occupancy sum hydrogen: 0 / Occupancy sum non hydrogen: 7716.1 | |||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 1.5→30 Å
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Refine LS restraints |
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