endonuclease activity / defense response to virus / Hydrolases; Acting on ester bonds / DNA binding / RNA binding / metal ion binding Similarity search - Function
Mass: 18.015 Da / Num. of mol.: 211 / Source method: isolated from a natural source / Formula: H2O
Sequence details
Sequence of AcrIIC2 was based on protein sequence of published literature (Pawluk et al., PMID: ...Sequence of AcrIIC2 was based on protein sequence of published literature (Pawluk et al., PMID: 27984730), in which reisdues 'MA' at terminal were reported.
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Experimental details
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Experiment
Experiment
Method: X-RAY DIFFRACTION / Number of used crystals: 1
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Sample preparation
Crystal
Density Matthews: 2 Å3/Da / Density % sol: 38.65 %
Crystal grow
Temperature: 293 K / Method: vapor diffusion, sitting drop / Details: 0.1M MES, pH 5.6, PEG 3000, PEG 200
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Data collection
Diffraction
Mean temperature: 193 K / Serial crystal experiment: N
Resolution: 1.78→43.41 Å / Cor.coef. Fo:Fc: 0.952 / Cor.coef. Fo:Fc free: 0.925 / SU B: 5.456 / SU ML: 0.079 / Cross valid method: FREE R-VALUE / ESU R: 0.249 / ESU R Free: 0.126 / Details: HYDROGENS HAVE BEEN USED IF PRESENT IN THE INPUT
Rfactor
Num. reflection
% reflection
Selection details
Rfree
0.22392
1259
4.9 %
RANDOM
Rwork
0.17725
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obs
0.1795
24512
94.87 %
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Solvent computation
Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å